In vitro immunomodulatory activity test of Bengle rhizoma extract (Zingiber cassumunar Roxb.): phagocytic activity of macrophages and lymphocyte proliferation in mice

Abstract

Immunomodulators are pharmacological agents that affect the immune system at different levels. Aside from modulating, some immunomodulators stimulate, while some others inhibit immune responses. Zingiber cassumunar Roxb. or Bengle rhizoma has been reported to exhibit immunomodulatory activities. This research was intended to determine the pharmacological effects of its extract on the phagocytic activity of macrophages and lymphocyte proliferation in vitro. It used the macrophages and lymphocytes of male BALB/c mice, which were divided into normal control and treatment group (receiving 25, 50, and 100 ppm of extract). The immunomodulatory activity test results showed that 100 ppm of Bengle rhizoma extract reduced phagocytosis in macrophages much significantly than the control group and that the treatment groups suppressed the proliferation of lymphocytes more substantially than the control group. The extract decreased the phagocytic activity of macrophages and the proliferation capacity of lymphocytes when administered at a concentration of 100 ppm.

Potential bangle (Zingiber montanum J.König) rhizome extract as a supplement to prevent and reduce symptoms of Covid-19

“…Then Adhila et al (2019) also conducted research using bangle rhizome extract with concentrations of 25, 50, and 100 ppm. Experiments were carried out with the macrophages and lymphocytes of male BALB/c mice.…”

“…The results of 3 kinds of phenylbutenoid compounds have immunomodulatory effects and the compounds [(E) -4- (3′, 4′-dimethoxyphenyl) but-3-en-1-ol] were the best. The immunomodulatory effect of this compound increases the activity and capacity of mouse macrophage cells in ingesting Staphilococcus epidermidis ( Adhila et al, 2019 ).…”

The effect of Zingiber cassumunar Roxb rhizome extract on in vitro phagocytic activity and lymphocyte proliferation

“…After a stopper reagent (10% SDS in 50 μl HCl 0.01 N) was added to each well, the microplate was stored at room temperature for 12 hours in a dark condition. Then, the absorbance values were assessed using an ELISA reader at a wavelength of 595 nm (Adhila et al, 2019).…”