In Vitro, In Vivo and Phytochemical Screening, of Extracts of Piper guineense for trypanocidal Activities Against Trypanosoma brucei brucei.

Abstract

Extracts from leaves and stem bark of Piper guineense were evaluated for trypanocidal activity against Trypanosoma brucei brucei in vitro and in vivo. The phytochemical screening of the most active extracts in vitro was carried out. The Petroleum ether extract of stem bark exhibited highest in vitro trypanocidal activity out of the six extracts tested. Thus it was used to treat animals infected with Trypanosoma brucei brucei at a dose range of 50-200 mg/kg body weight, intraperitoneally and orally. The results showed that the group treated with 100 mg/kg body weight intraperitoneally showed significant reduction in parasiteamia with a prolonged life span up to the 18th day post infection. While those that were treated with 200 mg/kg body weight orally, had progressive increase in parasiteamia up to the 7th and 8th day post infection when they all died. However in the prophylactic experiment animals that were treated with 50 mg/kg intraperitoneally and 100 mg/kg orally for five days before challenging them with 10 3 trypanosomes, did not develop parasiteamia up to 28th day post infection when the experiment was terminated. This experiment shows that the petroleum ether of Piper guineense possesses some degree of trypanocidal activity and further purification of the active component should be carried out.

In vitro antiprotozoan activity and mechanisms of action of selected Ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites

“…This study reports the anti‐ Trypanosoma activity of three of the 10 plant species, C. cordifolia , N. laevis , and P. angolensis , for the first time. The anti‐ Trypanosoma activity of the remaining seven plant species have been reported in previous studies (Abedo et al, ; Bero, Hannaert, Chataigné, Hérent, & Quenti‐Leclercq, ; Ganfon et al, ; Kimani, Gathumbi, Auma, Ngeranwa, & Masiga, ; Mann et al, ; Shuaibu et al, ). The possible mechanisms of action are however unknown.…”

“…We previous studies (Abedo et al, 2013;Bero, Hannaert, Chataigné, Hérent, & Quenti-Leclercq, 2011;Ganfon et al, 2012;Kimani, Gathumbi, Auma, Ngeranwa, & Masiga, 2013;Mann et al, 2011;Shuaibu et al, 2008). The possible mechanisms of action are however unknown.…”

Effective Factors in the Chemical and Biological Control of Fasciola spp. With an Emphasis on the Effects of Macrofungi

“…In 1970, Barnett Cline used meteorological information to achieve epidemiological goals, and today, using satellite information to predict disease status is one of the common methods in disease epidemiology (6).…”