In vitro infection of cells of the monocytic/macrophage lineage with bovine leukaemia virus

CD4 T Lymphocyte Activation in BLV-Induced Persistent B Lymphocytosis in Cattle

“…Although several studies indicate that peripheral blood monocytes can be infected by BLV (6,19,20), our results failed to provide evidence that monocytes accounted for the PBMC accessory cell stimulation of CD4 T lymphocytes. It could be that BLV-infected monocytes or B lymphocytes alone, or their interactions with other PBMC subsets in the accessory cell population, activate CD4 T lymphocytes and that our purification procedures interfered with this by changing functional characteristics of cells.…”

“…6B). Because BLV may also infect monocytes (6,19,20), additional experiments were conducted in which adherent cells were added back to PBMC or T lymphocyte accessory cell preparations. Although these monocyte-enriched accessory cells could stimulate CD4 T lymphocytes above background levels, proliferation did not correlate with the percentage of monocytes present, nor did monocyte enrichment of PBMC and T lymphocyte accessory cell preparations ever result in proliferation levels equivalent to PBMC accessory cell stimulation (data not shown).…”

Avaliação funcional de monócitos de bovinos naturalmente infectados pelo vírus da leucose bovina

“…Desta forma, isso pode explicar a menor viabilidade dos monócitos observada no presente estudo. De fato, Domenech et al (2000), ao infectarem macrófagos bovinos in vitro, observaram que, embora apresentassem poucas alterações estruturais, tais células expressavam diversas proteínas virais, indicando replicação viral.…”

Genetic variation of bovine leukemia virus (BLV) after replication in cell culture and experimental animals

“…The major viral targets are B-lymphocytes, which express surface immunoglobulin M (Schwartz et al, 1994;Mirsky et al, 1996); however, BLV has been reported to infect other cell lineages in vivo, such as CD8 + T cells, monocytes, and granulocytes (Schwartz et al, 1994;Domenech et al, 2000). In addition, BLV has been found to infect a wide variety of cells in vitro, such as fetal lamb kidney (FLK), feline fibroblast (CC81), Madin-Darby bovine kidney (MDBK), baby hamster kidney (BHK), and human epithelial cervical cancer (HeLa) (Graves and Ferrer, 1976;Ferrer et al, 1981;Altaner et al, 1989;Inabe et al, 1998).…”