In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A

Li, Xiaoping; Wang, Jiye; Ping, Gou; Mao, Cungui; Zhu, Zhiliang; Li, Hongye

doi:10.1016/j.ijfoodmicro.2007.07.054

Cited by 57 publications

(33 citation statements)

References 27 publications

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“…3). These are consistent with the previous findings that AbA can alter normal bud growth, delay conidial germination initiation, restrict the elongation of the germ tube and mycelium, as well as induce abnormal alternations in the morphology of fungi (Endo et al, 1997;Liu et al, 2007). In contrast, after treatment with AbA, the cell mor- A, with wild-type; B, with wild-type plus 2 µg/ml AbA; C, with BcAUR1a; D, with BcAUR1a plus 2 µg/ml AbA; E, with BcAUR1b; F, with BcAUR1b plus 2 µg/ml AbA.…”

Section: Discussionsupporting

confidence: 93%

“…The plates were irradiated with UV for 1 min and incubated in the dark for 30 s, this step was repeated for 4-5 times, and then incubated in the dark for 90 min and finally cultivated for 4 days at 28°C. Spore culture was performed as described by Liu et al (2007). AbAresistant conidia were obtained by single spore isolation and selected on a PDA medium with AbA at concentrations of 4, 8, 20, 50 and 80 µg/ml.…”

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

“…Spores of wild-type and AbA-resistant mutants of B. cinerea (2 × 10 5 conidia per ml) were cultivated on a PDA medium in AbA-free or 8 µg/ml AbA, respectively. Fungal culture and the observation of cell morphology were performed as described by Liu et al (2007). About 50 conidia and germ tubes were examined microscopically.…”

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

“…There were three replicates with 10 fruits per replicate and the experiment was conducted twice. The experiment was performed according to the method given by Liu et al (2007).…”

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

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Characteristics of inositol phosphorylceramide synthase and effects of aureobasidin A on growth and pathogenicity of <i>Botrytis cinerea</i>

Wang

Guo

et al. 2015

J. Gen. Appl. Microbiol.

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IntroductionSphingolipids are known to be important components Inositol phosphorylceramide (IPC) synthase is the key enzyme with highly conserved sequences, which is involved in fungal sphingolipid biosynthesis. The antibiotic aureobasidin A (AbA) induces the death of fungi through inhibiting IPC synthase activity. The mutations of AUR1 gene coding IPC synthase in fungi and protozoa causes a resistance to AbA. However, the mechanism of AbA resistance is still elusive. In this paper, we generated two mutants of Botrytis cinerea with AbA-resistance, BcAUR1a and BcAUR1b, through UV irradiation. BcAUR1a lost an intron and BcAUR1b had three amino acid mutations (L197P, F288S and T323A) in the AUR1 gene. AbA strongly inhibits the activity of IPC synthase in wild-type B. cinerea, which leads to distinct changes in cell morphology, including the delay in conidial germination, excessive branching near the tip of the germ tube and mycelium, and the inhibition of the mycelium growth. Further, AbA prevents the infection of wild-type B. cinerea in tomato fruits via reducing oxalic acid secretion and the activity of cellulase and pectinase. On the contrary, AbA has no effect on the growth and pathogenicity of the two mutants. Although both mutants show a similar AbA resistance, the molecular mechanisms might be different between the two mutants.

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Section: Discussionsupporting

confidence: 93%

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

“…There were three replicates with 10 fruits per replicate and the experiment was conducted twice. The experiment was performed according to the method given by Liu et al (2007).…”

Section: Strains and Chemicals B Cinerea-t-4-56mentioning

confidence: 99%

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Characteristics of inositol phosphorylceramide synthase and effects of aureobasidin A on growth and pathogenicity of <i>Botrytis cinerea</i>

Wang

Guo

et al. 2015

J. Gen. Appl. Microbiol.

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“…The antifungal activities of different fungicides on citrus against P.digitatum and P.italicum were determined by mycelial growth assays (Liu et al, 2007). The antifungal activities were determined in vitro by transferring plugs (5 mm in diameter) of mycelium from the leading edge of an actively growing colony to a series of PDA (potato dextrose agar) plates containing 0.5, 1.0, 2.0, 4.0 and 8.0 μg/mL fungicides.…”

Section: Inhibition Effects Of the Fungicides On P Digitatum And P mentioning

confidence: 99%

Preservative Effects of Strobilurin Fungicides on Citrus Storage Diseases and Residue Safety Assessment

Song¹,

Chen²,

Chen³

et al. 2018

JAS

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Ethofenprox, pyraclostrobine, trifloxystrobin and enestroburin are strobilurin fungicides that can effectively control fungal diseases caused by ascomycetes, zygomycetes, imperfect fungi, etc. With the purpose of guaranteeing the safe use of strobilurin fungicides in the prevention and treatment of citrus diseases, toxicity and control effects of the fungicides on citrus storage diseases and GC-MS (gas chromatography-mass spectrometry) were applied in this study to determine its residual safety. The results indicated that ethofenprox, trifloxystrobin, pyraclostrobine, and enestroburin had excellent inhibitory effects on citrus storage diseases at concentration of 200-400 μg/mL. Degradation dynamics of 4 fungicides during the storage period of citrus could be expressed as the first-order kinetics equation. The fungicides could penetrate into flesh through peels slowly. Therefore, the residue content of the fungicide on peels was higher than that in the flesh of the same citrus. After citrus fruits were treated for 90 d, the residues were lower than the maximum residue limits in all the countries, so the citrus were safe. This investigation provided the theoretical guidance and technical support for the quality evaluation of citrus products.

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Dissecting the Mechanisms of Action of Biocontrol Agents to Control Postharvest Diseases of Fruit

Spadaro

2014

Biological Controls for Preventing Food Deterioration

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In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A

Cited by 57 publications

References 27 publications

Characteristics of inositol phosphorylceramide synthase and effects of aureobasidin A on growth and pathogenicity of <i>Botrytis cinerea</i>

Characteristics of inositol phosphorylceramide synthase and effects of aureobasidin A on growth and pathogenicity of <i>Botrytis cinerea</i>

Preservative Effects of Strobilurin Fungicides on Citrus Storage Diseases and Residue Safety Assessment

Dissecting the Mechanisms of Action of Biocontrol Agents to Control Postharvest Diseases of Fruit

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