In vitro inhibition of the biosynthesis of slow reacting substance of anaphylaxis (SRS-A) and lipoxygenase activity by quercetin

Hope, William C.; Welton, Ann F.; Fiedler‐Nagy, Christa; Batula-Bernardo, C.; Coffey, John W.

doi:10.1016/0006-2952(83)90569-5

Cited by 142 publications

(40 citation statements)

References 13 publications

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“…The vast majority of medicinal plants did not exhibit 5-lipoxygenase inhibitory activity. Only seven extracts exhibited dose-dependent 5-lipoxygenase inhibitory activity, with the aqueous extract of Melianthus comosus displaying the most potent activity with an IC 50 value of 13.84 ppm, while NDGA, which represented the positive control, had a reported IC 50 value of 5 ppm [19][20][21] . The essential oil of B. africana displayed 5-lipoxygenase inhibitory activity with an IC 50 value of 29.99 ppm.…”

Section: Resultsmentioning

confidence: 99%

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

Frum

Viljoen

2006

Skin Pharmacol Physiol

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An investigation was undertaken to determine the possible mechanisms of action of medicinal plants used for dermatological pathologies. A total of 14 plant species were selected from the readily available ethnobotanical literature. 5-Lipoxygenase and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays were used to determine the anti-inflammatory activity and the anti-oxidant activity of selected medicinal plants, respectively. Both aqueous and methanol extracts were tested. Among the plants screened, four species (Croton sylvaticus, Warburgia salutaris, Pentanisia prunelloides, and Melianthus comosus) displayed promising 5-lipoxygenase inhibitory activity with IC₅₀ values <61 ppm. A large number of plants exhibited significant anti-oxidant activities with IC₅₀ values between 5.27 and 83.36 ppm. Aqueous extracts of M. comosus exhibited the most potent anti-inflammatory and anti-oxidant activity.

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Section: Resultsmentioning

confidence: 99%

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

Frum

Viljoen

2006

Skin Pharmacol Physiol

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“…However, indomethacin and NDGA had a marginal inhibitory effect ( Figure 4B); even the concentrations of these 2 inhibitors were much higher than their IC 50 . 30,31 The inhibition of the flow-induced GALmPPAR␥-LBD and PPREϫ3-TK-Luc by 1-ABT suggests that the activation and ligand production of PPAR␥ by laminar flow are mediated through CYP450s but not COXs or LOXs. Although 15d-PGJ 2 is a potent PPAR␥ ligand, its affinity for PPAR␥ is low (EC 50 Ϸ2 mol/L).…”

Section: Discussionmentioning

confidence: 99%

Laminar Flow Activates Peroxisome Proliferator-Activated Receptor-γ in Vascular Endothelial Cells

et al. 2004

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Background-Steady laminar flow is atheroprotective, in part because of its antiinflammatory effects on vascular endothelial cells (ECs). We studied the activation of peroxisome proliferator-activated receptor-␥ (PPAR␥) in ECs in response to laminar flow and the associated antiinflammatory effect. Methods and Results-Using flow channel with cultured ECs, we found that laminar flow activated the PPAR␥-mediated PPAR-responsive element (PPRE) activity and increased the mRNA encoding CD36, a PPAR␥-targeted gene. Analysis of the CD36 promoter revealed that PPRE was required for flow activation. Laminar flow induced the GAL-PPAR␥-LBD fusion protein, which suggests that flow activation of PPAR␥ was ligand dependent. The pharmaceutical inhibitors of phospholipase A2 (PLA2) and cytochrome P450 epoxygenases (CYP450s) were able to block the laminar flow-activated PPAR␥. We also showed that lipid extracts from flow media contained ligands for the activation of PPAR␥ in other cell types. This paracrine activation exerted antiinflammatory effects in ECs and THP-1 cells, including the suppression of cytokine-induced nuclear factor-B activation and expression of intercellular adhesion molecule-1. Key Words: cells, endothelial Ⅲ receptors, peroxisome proliferator-activated Ⅲ inflammation D epending on the geometry of the arterial tree, blood flow in the straight part of vessels is steady and laminar, whereas that in the bends and branches is disturbed. 1 The focal distribution of atherosclerotic lesions in the disturbed flow areas indicates the pivotal role of local flow in atherogenesis. 2 Because early atherosclerotic events involve the inflammatory responses of vascular endothelial cells (ECs), disturbed flow patterns with high shear stress gradients are considered to be proinflammatory, whereas steady laminar flow is antiinflammatory. 3,4 Using flow channel systems, we and others demonstrated that the application of flow to cultured ECs transiently induced genes encoding chemoattractants, adhesion molecules, and cytokines, which is due in part to the activation of nuclear factor-B (NF-B) and AP-1. 5,6 The induction of these genes has been suggested to be the endothelial response to the rapid change of shear stress (/t). 7 In agreement with this hypothesis, prolonged laminar flow suppresses the expression of proinflammatory molecules. 8 To date, the molecular basis underlying the antiinflammatory effect of laminar flow is still elusive. Conclusions-LaminarPeroxisome proliferator-activated receptor (PPAR) -␣, -␤/␦, and -␥ constitute a subfamily of nuclear receptors. Among PPARs, PPAR␥ is largely expressed in monocytes/ macrophages, adipocytes, and intestinal cells in which PPAR␥ regulates genes involved in cell differentiation and lipid uptake and storage (eg, aP2, CD36). 9 The ligand-binding domain (LBD) of PPAR␥ can bind a wide range of ligands that have distinct structures, 10 including the natural ligand 15-deoxy-⌬ 12,14 -prostaglandin J 2 (15d-PGJ 2 ) and synthetic ligand thiozolidinediones (TZDs). PPAR␥ is also present in...

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“…One of their effects is the prevention of the atherogenic oxidation of cholesterol-LDL (Steinberg et al, 1989). However, flavonols and flavones can also inhibit the lipoxygenase (Hope et al, 1983), decreasing the platelet aggregation and reducing thrombocitic tendencies (Gryglewski et al, 1987 andTzeng et al, 1991). Jahromi and Ray (1993) observed that pterosupin and liquirritigenin were able to reduce cholesterol, cholesterol-LDL and triacylglycerol levels in Triton-induced hyperlipemic rats.…”

Section: Introductionmentioning

confidence: 99%

Effect of flavonoids morin; quercetin and nicotinic acid on lipid metabolism of rats experimentally fed with triton

Ricardo

Oliveira

Nagem

et al. 2001

Braz. arch. biol. technol.

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Atherosclerosis is a coronary disease where deposition of lipids in the arteries leads to problems of blood circulation. The present work evaluates the action of the flavonoids morin, quercetin and nicotinic acid isolated in association on lipid metabolism in hyperlipidemic rats. Blood serum levels cholesterol, cholesterol-HDL, and triacylglycerids have been analysed following the intraperitoneal administration of the flavonoid compounds dissolved in propyleneglycol by in doses of

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In vitro inhibition of the biosynthesis of slow reacting substance of anaphylaxis (SRS-A) and lipoxygenase activity by quercetin

Cited by 142 publications

References 13 publications

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

Laminar Flow Activates Peroxisome Proliferator-Activated Receptor-γ in Vascular Endothelial Cells

Effect of flavonoids morin; quercetin and nicotinic acid on lipid metabolism of rats experimentally fed with triton

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