In-vitro-Kultivierung von Zellen der respiratorischen Schleimhaut auf Matrizes aus Kollagen, Poly-L-Laktid (PLLA) und Polyhydroxybuttersäure (PHB)

doi:10.1055/s-2003-43238

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…Moreover, we observed vectorial release of the neutrophil chemokine, IL-8, in response to TNFα. While other studies have tested the utility of PLLA membranes, these have usually been limited to demonstration of their ability to support growth of human cells, including respiratory epithelial cells [36], to date, there has been little functional assessment of biological barrier properties [26, 27, 37]. For example, Selvam et al used a solvent-cast/particulate leaching technique to fabricate microporous PLLA membranes from PLLA/polyethylene glycol blends and confirmed permeation of glucose, L-tryptophan, and dextran, as well as growth of lacrimal acinar cells which retained histiotypic morphological and physiological characteristics of in vivo .…”

Section: Discussionmentioning

confidence: 99%

Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes

et al. 2019

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Development of biocompatible and functional scaffolds for tissue engineering is a major challenge, especially for development of polarised epithelia that are critical structures in tissue homeostasis. Different in vitro models of the lung epithelial barrier have been characterized using non-degradable polyethylene terephthalate membranes which limits their uses for tissue engineering. Although poly-L-lactic acid (PLLA) membranes are biodegradable, those prepared via conventional Diffusion Induced Phase Separation (DIPS) lack open-porous geometry and show limited permeability compromising their use for epithelial barrier studies. Here we used PLLA membranes prepared via a modification of the standard DIPS protocol to control the membrane surface morphology and permeability. These were bonded to cell culture inserts for use in barrier function studies. Pulmonary epithelial cells (H441) readily attached to the PLLA membranes and formed a confluent cell layer within two days. This was accompanied by a significant increase in trans-epithelial electrical resistance and correlated with the formation of tight junctions and vectorial cytokine secretion in response to TNFα. Our data suggest that a structurally polarized and functional epithelial barrier can be established on PLLA membranes produced via a non-standard DIPS protocol. Therefore, PLLA membranes have potential utility in lung tissue engineering applications requiring bio-absorbable membranes.

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Section: Discussionmentioning

confidence: 99%

Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes

et al. 2019

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“…Treatment of PHB films with NaOH also led to a 25-fold increase in the viable cell number compared with untreated PHB film[61]. It was shown that treatment of PHB film surfaces with low-pressure ammonia or oxygen plasma improved the growth of human fibroblasts, epithelial cells of respiratory mucosa, and rat bone marrow stromal osteoblastic cells (rat bone marrow MSCs) due to increased hydrophilicity (but with no change in microstructure) of the polymer surface[17,22,62]. It was suggested that the improved hydrophilicity of the films after PHB treatment with lipases, alkali, and plasma could allow cells in suspension to more easily attach to the polymer films compared with untreated films.…”

Section: Role Of Properties Of Phas As Natural Biopolymers In Their Ementioning

confidence: 99%

Effect of poly(3-hydroxyalkanoates) as natural polymers on mesenchymal stem cells

Voinova

Бонарцева

Бонарцев

2019

WJSC

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Mesenchymal stem cells (MSCs) are stromal multipotent stem cells that can differentiate into multiple cell types, including fibroblasts, osteoblasts, chondrocytes, adipocytes, and myoblasts, thus allowing them to contribute to the regeneration of various tissues, especially bone tissue. MSCs are now considered one of the most promising cell types in the field of tissue engineering. Traditional petri dish-based culture of MSCs generate heterogeneity, which leads to inconsistent efficacy of MSC applications. Biodegradable and biocompatible polymers, poly(3-hydroxyalkanoates) (PHAs), are actively used for the manufacture of scaffolds that serve as carriers for MSC growth. The growth and differentiation of MSCs grown on PHA scaffolds depend on the physicochemical properties of the polymers, the 3D and surface microstructure of the scaffolds, and the biological activity of PHAs, which was discovered in a series of investigations. The mechanisms of the biological activity of PHAs in relation to MSCs remain insufficiently studied. We suggest that this effect on MSCs could be associated with the natural properties of bacteria-derived PHAs, especially the most widespread representative poly(3-hydroxybutyrate) (PHB). This biopolymer is present in the bacteria of mammalian microbiota, whereas endogenous poly(3-hydroxybutyrate) is found in mammalian tissues. The possible association of PHA effects on MSCs with various biological functions of poly(3-hydroxybutyrate) in bacteria and eukaryotes, including in humans, is discussed in this paper.

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“…Since silicone obturators can not completely resolve perforation specific problems [69] the adaptation of such implants might be achieved by implantation of artificial polymer matrices which are seeded with mucosa specific epithelial cells to facilitate adaptation to the biological tissue and at the same time provide a scaffold and mechanical support. Numerous in vitro investigations with respiratory epithelial cells were focused on questions concerning the function of differentiated and undifferentiated cells and to cultivate respiratory epithelial cells on various artificial matrices [70], [71], [72]. In animal experimental models the implantation of membranes or moulds made of various polymers such as polyethylene [73], polypropylene [74], polyetherurethanes [75], polytetrafluorethylene [76], collagen [77] and polypropylene/collagen [78] has been reported.…”

Section: Selected Biomaterials Applications In Otolaryngologymentioning

confidence: 99%

Current requirements for polymeric biomaterials in otolaryngology

Sternberg¹

2009

GMS Current Topics in Otorhinolaryngology - Head and Neck Surgery; 8:Doc11; ISSN 1865-1011

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In-vitro-Kultivierung von Zellen der respiratorischen Schleimhaut auf Matrizes aus Kollagen, Poly-L-Laktid (PLLA) und Polyhydroxybuttersäure (PHB)

Abstract: In principle the analysed materials are useful for the culturing of cells from respiratory mucosa. However, for obtaining differentiated epithelial cells the culture conditions have to be modified.

Cited by 15 publications

References 18 publications

Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes

Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes

Effect of poly(3-hydroxyalkanoates) as natural polymers on mesenchymal stem cells

Current requirements for polymeric biomaterials in otolaryngology

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