In vitro maturation and fertilization of vitrified immature human oocytes, subsequent vitrification of produced embryos, and embryo transfer after thawing

Asimakopoulos, Byron; Kotanidis, Lazaros; Nikolettos, Nikos

doi:10.1016/j.fertnstert.2010.12.053

Cited by 10 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are few studies comparing the vitrification process before and after IVM. It has been demonstrated that vitrified immature oocytes can undergo post-warming in vitro maturation and fertilization, and also produce embryos able to undergo vitrification and warming [ 33 ] without generating chromosomal abnormalities [ 3 ]. Related to the SR of vitrified oocytes before and after IVM, similar SR have been reported for MII (86.9 %) and GV-oocytes (84 %) [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

Molina

Gómez

Balasch

et al. 2016

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundDuring cytoplasmic oocyte maturation, Ca2+ currents are vital for regulating a broad range of physiological processes. Recent studies have demonstrated that DMSO and EG cause large transient increases in intracellular Ca2+ in mouse oocytes. The CP used in vitrifying protocols also increases the intracellular calcium transient. The aim of this study is to evaluate the effects of vitrifying time (before and after IVM) and exposure to the vitrification solutions and ionomycin on oocyte quality and embryonic development.Methods221 GV-oocytes unsuitable for IVF-ICSI cycles were randomly distributed into one of the following three groups. G1 (control group): 41 GV-oocytes IVM until MII; G2: 43 oocytes vitrified at GV stage and IVM until MII stage; and G3: 53 GV-oocytes IVM until MII and then vitrified. In order to clarify the effect of vitrification solutions (VS) on human oocyte IVM through the intracellular Ca2+ oscillation, the following two groups were also included. G4: 43 GV-oocytes exposed to VS and IVM until MII; and G5: 41 GV-oocytes exposed to ionomycin and IVM until MII. All GV-oocytes that reached MII-stage were parthenogenetically activated to assess oocyte viability. IVM was performed in IVF-medium (24–48 h). Chemical treatment (ionomycin) and osmotic treatment (vitrification solutions) were performed without liquid-N2 immersion. The following rates were evaluated: survival (SR), in-vitro maturation (IVMR), activation (AR), development to 2-cell (DRC), development to morula (DRCM) and development to blastocyst (DRB). Ratios between the different treatment groups were compared using contingency tables analysis (chi-square test).ResultsA high survival rate was obtained in G2 (95.5 %) and G4 (96.6 %). In-vitro maturation rate was significantly higher for G4 (86 %) and G2 (83.7 %) compared to G1 (63.4 %), G3 (56.6 %) and G5 (48.8 %). DRCM was significantly higher for G1 and G2 compared to G3 (G1: 15.8 %, G2: 20.7 % and G3: 0 %). DRB was only obtained for the oocytes vitrified before IVM (G2: 3.4 %). AR was also significantly higher for G2 and G4 compared to G5 (G2: 80.5 %, G4: 86.5 % and G5: 55 %). DRCM and DRB were only obtained in G2 and G4. DRCM was significantly higher for oocytes vitrified at GV stage (G2) and for oocytes exposed to the VS in G4 compared to the oocytes exposed to the ionomycin in G5 (G2: 20.7 %; G4: 37.5 % and G5: 0 %).ConclusionsVitrifying GV-oocytes improves their IVM. Further investigation could look to increase the oocyte pool and improve fertility preservation options.

show abstract

Section: Discussionmentioning

confidence: 99%

Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

Molina

Gómez

Balasch

et al. 2016

Reprod Biol Endocrinol

View full text Add to dashboard Cite

show abstract

“…Our study has been performed with a less optimal material (failed-matured oocytes) and results are not comparable as those obtained in PCOS patients. Recently, Asimakopoulos et al [31] reported the efficiency of vitrified immature human oocytes before undergoing in vitro maturation, fertilization, and embryo development. For two women (34 and 36 years old) undergoing IVF cycle, immature oocytes were vitrified.…”

Section: Discussionmentioning

confidence: 99%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification. Methods A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2). Results Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p<0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p<0.05). Conclusion IVM procedure is more efficient when it is performed before oocyte vitrification.

show abstract

“…Improvements in the developmental competence and quality of embryos are still possible, however. Over the past several years, in vitro fertilization (IVF) of porcine and human eggs to generate embryos has been reported (Somfai et al, ; Asimakopoulos et al, ), and porcine and human in vitro embryo transplantation has been described (Abeydeera, 2001; Sazonova et al, ).…”

Section: Introductionmentioning

confidence: 99%

Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro

Zhang

Jiang

Lin

et al. 2015

Molecular Reproduction Devel

View full text Add to dashboard Cite

Lysophosphatidic acid (LPA), a member of the phospholipid autacoid family, is present in human follicular fluid. The aim of the present study was to compare the developmental competence of porcine embryos created via in vitro fertilization (IVF) and parthenogenetic activation (PA) in culture medium supplemented with LPA, in comparison with a control group. The effects of LPA on porcine oocyte maturation and pre-implantation embryonic development were also examined. Addition of 10 μM LPA to the oocyte maturation medium significantly increased the proportion of oocytes reaching metaphase I (MI) or metaphase II (MII), and enhanced embryonic developmental potential. When present during oocyte maturation, LPA significantly increased the abundance of phosphorylated ERK1/2 in MI and MII oocytes, showing that LPA enhanced nuclear maturation via activation of the mitogen-activated protein kinase (MAPK) pathway. In addition, Cyclin B1 levels were elevated in MI- and MII-stage oocytes, suggesting that LPA plays a role in both nuclear and cytoplasmic maturation of oocytes. After fertilization, the frequency of polyspermy in embryos obtained using LPA-treated oocytes was less than that in the control group. Further, blastocyst formation and blastocyst cell number were enhanced and apoptosis was reduced upon LPA treatment of embryos created either by IVF and PA. LPA treatment of blastocysts derived by IVF or PA resulted in increased expression of the anti-apoptotic BCL2L1 gene while reducing expression of the pro-apoptotic genes BAX and CASP3. Together, our data indicate that LPA supplementation improves porcine oocyte maturation and subsequent in vitro development of pre-implantation embryos.

show abstract

In vitro maturation and fertilization of vitrified immature human oocytes, subsequent vitrification of produced embryos, and embryo transfer after thawing

Cited by 10 publications

References 14 publications

Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

In-vitro maturation of human oocytes: before or after vitrification?

Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro

Contact Info

Product

Resources

About