In vitro Micrografting of Mature Pistachio (Pistacia vera var. Siirt)

In VitroMicrografting of the Almond Cultivars “Texas”, “Ferrastar” and “Nonpareil”

“…the increased shoot length in the hypocotyls may be due to cambial contact and absence of competition from axillary buds that are present in the epicotyls. Although several authors have previously reported that in the micrografting of different plant species, e.g., cashew (34), pistachio (26), cherry (4) and King Protea (36), including almond (13), the size of the scion affects the success rate of micrografts, no significant difference in the frequency of successful micrografts was obtained in the three of the almond cultivars tested when the microscion was applied to different sizes. Fig.…”

“…overall, the molecular analysis showed that the genetic instability of the micrografted clones, although with a small percentage, started after 6 months of subculturing in cvs "Ferrastar" and "nonpareil". Effects of scission type, rootstock type and media on the micrografts Micrografting was applied successfully to some woody species such as Pistacia vera onay et al (26), Hevea brasiliensis Perrin et al (28) and Quercus ewald and naujoks (9) and some almond cultivars, i.e. (8,13,20,37), for the rejuvenation of adult tissues.…”

“…In vivo micrografting consists of grafting a relatively small scion originating from a desirable mother plant onto a young rootstock obtained either from a seedling or from an herbaceous cutting grown in a nursery or under aseptic conditions. on the other hand, in the case of in vitro micrografting the scion is surface sterilized before use and the rootstock is either a seedling obtained from aseptically germinated seed or a micropropagated microcutting (26,27).…”

Morphological and Anatomical Investigations on Old Home x Farmingdale 333 / Pyrus elaeagrifolia in Vitro Micrografts

“…Grafting techniques and initial treatments applied prior to in vitro micrograftings are subjects of concern. Cleft grafting was used in this study because it has been reported as a successful micrografting technique in different fruit species such as cherry, pistachio, olive (Ozzambak & Schmidt 1991;Abousalim & Mantell 1992;Toroncoso et al 1999;Onay et al 2004). …”

Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

“…This reinvigoration includes anatomical, molecular and epigenetic changes which reflect characteristic juvenile individual's protein patterns, DNA methylation and polyamine content [6] [9]. Altogether, the reversion of several adult phenotypic traits towards more juvenile stages and the recovery of tissue morphogenic capabilities allow the cloning of selected adult trees [6] [9] [10]. One tissue reinvigoration techniques is the in vitro micrograft, which has been tested to reinvigorate ontogenetically adult vegetative buds on juvenile rootstocks [11] and can be a solution for cloning adult trees of several species, in which sprouts present deficient rooting and lack of vigor [3] [4].…”