In vitro micrografting using three diverse indigenous rootstocks for the production of Citrus tristeza virus-free plants of Khasi mandarin

Singh, Amit; Meetei, Ng. Tombisana; Kundu, Suprabuddha; Salma, Umme; Mandal, Nirmal

doi:10.1007/s11627-018-9946-6

Cited by 19 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, a further increment of graft success (to 38%) was found in Kinnow mandarin grown in culture medium supplemented with 70 g L −1 sucrose [96]. Similar improvements in successful micrografting for other Citrus species, by increasing sucrose concentration in the culture medium, have been reported by Navarro et al [11,62], Ali et al [40], and Singh et al [97].…”

Section: Culture Conditionssupporting

confidence: 57%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

View full text Add to dashboard Cite

In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

show abstract

Section: Culture Conditionssupporting

confidence: 57%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Moreover, this is the rst attempt in kiwifruit in vitro micrografting where we use the rootstock without root grown in light with signi cant success rate. Rootstock without root further simpli es the handling process and can be grown easily in agar medium without the need to grow the graft in liquid media (Singh et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Establishment of a n efficient method for kiwifruit in vitro micrografting

Yao

Kong²,

Lei

et al. 2022

Preprint

View full text Add to dashboard Cite

This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of kiwifruit ‘Miliang-1’ and ‘Hongyang’ was used as rootstock and scion. Accomplishment of in vitro grafting has been examined by varying numerous factors, the physiological state of the rootstock and scion, the pH value of the medium, the concentration of sucrose, the type of medium and the PGRs. The results showed that the best condition of kiwifruit in vitro grafting was that the rootstock does not take root, and both the rootstock and scion have no leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid media along with 0.5 mg L− 1 GA3, 1.0mg L− 1 IBA, 40 g L− 1 sucrose and 7 g L− 1 agar, pH 6.0. The matrix formula of perlite: peat soil: vermiculite volume ratio of 1:2:1 was the most suitable substrate for transplanting micrografted seedlings.

show abstract

“…These may result in the diversity of virus eradiation. Singh et al (2019) have reported that the survival rate for in vivo micrografting with shoot tip size of 0.3-0.5 mm was 42.0% and all micrografted 'Khasi' mandarin plants showed negative for CTV via RT-PCR test. It has been suggested that the frequency for virus elimination depends on the concentration of target virus in shoot apex and the physiological condition of the mother plant (Verma et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Elimination of Citrus Tristeza Virus from Triploid Pummelo Cultivar [Citrus maxima (Burm.) Merr.] by In Vivo Micrografting on Seedlings of Three Citrus Cultivars

Le¹,

Sakai²,

Mizunoe³

et al. 2021

Journal of the Faculty of Agriculture, Kyushu University

View full text Add to dashboard Cite

Seedlessness in Citrus cultivars is one of the important characters accepted by consumers. Triploid pummelo contains no or a few seeds even though they are planted in a mixed block. Four triploid pummelo hybrids bred in Kyushu University are in the course of registration for new seedless cultivars release. Citrus tristeza virus (CTV) may be the catastrophic cause of decline of the production when the new pummelo cultivars release and expand. Therefore, virus test for the original trees and production of virus-free clones before the release are necessary. Micrografting with shoot apices of scions on the epicotyls of decapitated seedlings is the best ways to remove viruses in Citrus. In this study, shoot tips with 2, 4 or 6 leaf primordia of CTV-infected triploid pummelo 112A were grafted in vivo on 2-week-old seedlings of 'Hiradobuntan' pummelo, 'Natsudaidai' tangelo and 'Variegated Daidai' sour orange rootstocks to seek suitable rootstock seedlings and produce CTV-free clones. It was verified from this study that the larger the shoot tip size, the higher the rate for success in in vivo micrografting. All of the shoots derived through in vivo micrografting of shoot tips with 2, 4 or 6 leaf primordia were determined to be CTV-negative through immunochromographic assay 9-12 months after micrografting; however, 26.3 % of the shoots showed positive for CTV through RT-PCR test. The resulting virus free shoots (73.7 %) will be vegetatively propagated and proliferated for cultivar release.

show abstract

In vitro micrografting using three diverse indigenous rootstocks for the production of Citrus tristeza virus-free plants of Khasi mandarin

Cited by 19 publications

References 22 publications

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

Establishment of a n efficient method for kiwifruit in vitro micrografting

Elimination of Citrus Tristeza Virus from Triploid Pummelo Cultivar [Citrus maxima (Burm.) Merr.] by In Vivo Micrografting on Seedlings of Three Citrus Cultivars

Contact Info

Product

Resources

About