In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Santander, Sonia; Alcaine, Clara; Lyahyai, Jaber; Pérez, M.Á.; Rodellar, Clementina; Doblaré, M.; Ochoa, Ignacio

doi:10.4012/dmj.2012-015

Cited by 13 publications

(11 citation statements)

References 50 publications

(36 reference statements)

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“…The performed investigations corroborate previous studies showing that each surface structure, such as trenches, ramparts and pointed structures, has an influence on cell adhesion, migration and proliferation [8,27,28,34]. Osteoblast-like cells are able to recognize the smallest structures of approximately 10 nm [6,18].…”

Section: Discussionsupporting

confidence: 91%

“…Early studies performed by Weiss already demonstrated that a microstructured surface can influence cell growth [36]. Today, current studies have shown that the surface topography as well as its chemical composition affects the interaction with the cells of the body in terms of cell adhesion, migration, proliferation and differentiation and production of specific proteins for bone formation [3,6,15,23,28,34].…”

Section: Introductionmentioning

confidence: 97%

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Adhesion of human mesenchymal stem cells can be controlled by electron beam-microstructured titanium alloy surfaces during osteogenic differentiation

Neuß¹,

Panfil

Campos

et al. 2015

Biomedical Engineering / Biomedizinische Technik

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Several studies focusing on bone tissue engineering demonstrated that given microstructuring of an implant surface has a strong effect on its interaction with cells, and their adhesion and differentiation. In the present study, geometrically structured titanium alloy surfaces are shown to be able to guide cell adhesion during differentiation in vitro. For this reason, using an electron beam texturing technique, TiAl6V4 surfaces were selectively targeted in the micrometer range. The effect of such textured titanium alloy surfaces on cell adhesion during osteogenic differentiation was analyzed for human mesenchymal stem cells (MSC), the natural precursor cells of bone tissue. Cytotoxicity, cell viability and differentiation were analyzed. Immunofluorescence stainings demonstrated that in contrast to MSC in an expansion medium, MSC in an osteogenic induction medium produce adhesion proteins such as ß3-integrins and thereby connect in an oriented way to the generated microstructures on titanium alloy surfaces. These results are of relevance for developing tailored titanium alloy implant surfaces which exhibit an improved cell response.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Adhesion of human mesenchymal stem cells can be controlled by electron beam-microstructured titanium alloy surfaces during osteogenic differentiation

Neuß¹,

Panfil

Campos

et al. 2015

Biomedical Engineering / Biomedizinische Technik

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“…It has been previously demonstrated that in vivo porous titanium implants have different biological behaviors: increased porosity and pore size showed a direct positive effect on the amount of new bone growth . Biomimetic modified titanium surfaces were important to improve cell adhesion, proliferation and osteogenic differentiation in the absence of any differentiation molecule . In vitro experiments demonstrated that a combination of surface properties like microstructure and high energy increased osteoblast maturation of MSCs or progenitor cells via autocrine and paracrine regulation .…”

Section: Discussionmentioning

confidence: 99%

Trabecular titanium can inducein vitroosteogenic differentiation of human adipose derived stem cells without osteogenic factors

Benazzo

Botta

Scaffino

et al. 2013

J Biomedical Materials Res

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Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 and 21 days from differentiation, the hASCs grown on TT scaffolds showed similar expressions of alkaline phosphatase (ALP) and Runx-2 both in the presence and in the absence of osteogenic factors, as well as at transcript and protein levels. hASCs cultured on monolayer in the presence of the medium obtained from the wells where hASCs/scaffold constructs were cultured in the absence of osteogenic factors differentiated towards the osteogenic phenotype: their gene and protein expression of ALP and Runx-2 was similar to that of the same cells cultured in the presence of osteogenic factors, and significantly higher than that of the ones cultured in growth medium.

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“…The metal has been recognized as nonbioactive material, which has no osteoinductive and osteoconductive properties. However, in last few decades, numbers of studies have demonstrated that some metals such as titanium can perform as bioactive materials through the specific treatments such as increasing osteobiological traits through refining surface roughness (Santander et al, ). Because materials can use their surface to interact with biological environment, controlling surface topography is a crucial parameter for determining its ion interchange, protein adhesion, and cell interaction (Tejero, Anitua, & Orive, ).…”

Section: Osteobiologic Materialsmentioning

confidence: 99%

Advances in osteobiologic materials for bone substitutes

Hasan

Byambaa

Morshed

et al. 2018

J Tissue Eng Regen Med

109

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A significant challenge in the current orthopedics is the development of suitable osteobiologic materials that can replace the conventional allografts, autografts, and xenografts and thereby serve as implant materials as bone substitutes for bone repair or remodelling. The complex biology behind the nanostructure and microstructure of bones and their repair mechanisms, which involve various types of chemical and biomechanical signalling amongst different cells, has set strong requirements for biomaterials to be used in bone tissue engineering. This review presents an overview of various types of osteobiologic materials to facilitate the formation of the functional bone tissue and healing of the bone, covering metallic, ceramic, polymeric, and cell-based graft substitutes, as well as some biomolecular strategies including stem cells, extracellular matrices, growth factors, and gene therapies. Advantages and disadvantages of each type, particularly from the perspective of osteoinductive and osteoconductive capabilities, are discussed. Although the numerous challenges of bone regeneration in tissue engineering and regenerative medicine are yet to be entirely addressed, further advancements in osteobiologic materials will pave the way towards engineering fully functional bone replacement grafts.

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In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Cited by 13 publications

References 50 publications

Adhesion of human mesenchymal stem cells can be controlled by electron beam-microstructured titanium alloy surfaces during osteogenic differentiation

Adhesion of human mesenchymal stem cells can be controlled by electron beam-microstructured titanium alloy surfaces during osteogenic differentiation

Trabecular titanium can inducein vitroosteogenic differentiation of human adipose derived stem cells without osteogenic factors

Advances in osteobiologic materials for bone substitutes

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