In Vitro Phytochemical and Antibacterial Studies on Rhinacanthus Nasutus (L.) Kurz - A Medicinally Important Plant

Abstract

The present study was aimed to evaluate and compare the phytochemical and antibacterial potential of mother plants (aerial parts), in vitro leaves and stems derived callus and in vitro callus mediated shootlets of Rhinacanthus nasutus (L.) Kurz. Preliminary phytochemical analysis and extraction was performed on stem and leaves segments derived calli (4 weeks old), aerial portions of mother plants and calli mediated shootlets of R. naustus. Anti-bacterial activity of different extracts (25 µg) (ethanol, chloroform, aqueous and ethyl acetate) of stem and leaves segments derived calli, aerial portions of mother plants and calli mediated shootlets were investigated by well-diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. The preliminary phytochemical studies confirmed that the leaves and stem derived calli mediated shootlets showed the higher degree of metabolite constituents and extraction value compared to the in vitro derived calli and in vivo leaves and stem (aerial portions). The ethyl acetate extracted solvents showed the maximum bio-efficacy compared with other solvents due to the presence of more compounds such as saponins, steroids, tannins, phenolics, triterpenoids, alkaloids, coumarins, anthraquinones, glycosides and flavonoids. The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. The methods developed in this work make possible for the low volume and high potential production of active principles under in vitro condition in short duration with less amount of explants utilization. .

Effects of Ethanolic Extract of Rhinacanthus Nasutus (L) Kurz as an Antimicrobial Against Escherichia Coli Bacteria Using in Vitro Method

“…Increased resistance of microorganisms to drugs was a global threat in therapy. This can be caused by the use of synthetic drugs that ware irregular and incompatible with the pathogens of bacteria, viruses, and fungi and the presence of several chemicals inhibiting the action of antimicrobial drugs leading to changes in the target and ability of drugs to penetrate across bacterial cell wall [9]. Extract of R. nasutus had maximum bio-efficacy compared to other solvents because it had more compounds such as saponins, steroids, tannins, phenolic, triterpenoids, more alkaloids, and flavonoids that were produced using in vitro method and were able to inhibit pathogenic bacteria [10].…”