In Vitro Plant Regeneration of Cymbopogon jwarancusa (Jones) Schult from Meristematic Base of Spikelet

Mehandru, Pooja; Gehlot, H. S.; Shekhawat, N. S.; Vibha, J. B.; Dinesh, Rachana

doi:10.1007/s40009-013-0210-7

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Misra and Datta (2001) reported direct organogenesis from leaf segments on MS medium supplemented with BAP (4.44 µM) and GA (14.43 µM), and Vanegas et al (2002) established an efficient protocol for regeneration via indirect organogenesis using IAA (17.1 µM) and BAP (13.3 µM), where the successful acclimatization and (Rathore et al 2015) and Solanum melongena with 2,4-D (0.5 mg l −1 ) (Ashraful-Habib et al 2016). Transition from dedifferentiated status to embryogenic and/or organogenic status is a complex process that consists of several phases, including cell reactivation, cell division and various metabolic and developmental reprogramming steps (Pooja Mehandru et al 2014). It has been reported that 2,4-D is the most widely used as a growth regulator for morphogenic calli induction regardless of the explant according to our results (Dey et al 2010;Rathore et al 2015).…”

Section: Induction and Somatic Embryo Formationsupporting

confidence: 54%

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

Espinoza

Benítez-García

Peralta

et al. 2017

Plant Biotechnology

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Tagetes erecta is an asteraceous plant of industrial, ornamental and medicinal importance; its inflorescences have been used as a pigment source for food coloring, mainly for poultry skin and eggs. Nevertheless, there are few reports on plant regeneration or micropropagation, because unsuccesfull results in the plant's reaction to the growth regulators, developing embryogenesis on Tagetes erecta. In this study, somatic embryogenesis was induced and plantlets of Tagetes erecta were regenerated. For induction of globular structures MS medium supplemented with 2,4-D (4.5 µM) and BAP (8.8 µM) was used; globular structures were transferred to MS medium with 45 g l −1 sucrose until the embryos maturation. Transmission electron microscopy showed characteristic subcellular structures of embryogenic callus. Somatic embryos were transferred to MS medium without plant growth regulators and whole plantlets were obtained. In vitro plants were successfully transplanted into a mixture of peat moss and vermiculite (1 : 1 v/v) under greenhouse conditions. In this study, somatic embryogenesis and plant regeneration system from foliar explants were established, an important requirement for performing genetic transformation events on Tagetes erecta.

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Section: Induction and Somatic Embryo Formationsupporting

confidence: 54%

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

Espinoza

Benítez-García

Peralta

et al. 2017

Plant Biotechnology

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“…In several species, somatic embryo induction was achieved using different auxins such as 2,4-D, NAA, IAA, for example Swietenia macrophylla King (Meliaceae) with 2,4-D (4.0 mg L −1 ) and kinetin (1.0 mg L −1 ) [ 42 ]; Cymbopogon jwarancusa (Jones) Schult. (Poaceae) with 2,4-D (18.1 μM) [ 43 ]; Musa acuminata Colla (Musaceae) with 2,4-D (4.5 μM) [ 44 ]; Salicornia brachiata Roxb. with 2,4-D (2.0 mg L −1 ) [ 45 ], and Solanum melongena L. (Solanaceae) with 2,4-D (0.5 mg L −1 ) [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L−1), Naphthaleneacetic Acid (NAA) (1 mg L−1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L−1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L−1) and NAA (1 mg L−1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L−1) and NAA (0.25, 0.50, and 1 mg L−1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L−1 of BAP and 1 mg L−1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L−1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

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In Vitro Plant Regeneration of Cymbopogon jwarancusa (Jones) Schult from Meristematic Base of Spikelet

Cited by 2 publications

References 11 publications

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

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