In vitro predatory activity of conidia of fungal isolates of the Duddingtonia flagrans on Angiostrongylus vasorum first-stage larvae

Braga, Fábio Ribeiro; Araújo, Juliana Milani; Araújo, Jackson Víctor de; Soares, Filippe Elias de Freitas; Tavela, Alexandre de Oliveira; Frassy, Luiza Neme; Lima, Walter dos Santos; Mozzer, Lanuze Rose

doi:10.1590/0037-86829612013

Cited by 13 publications

(8 citation statements)

References 14 publications

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“…14 In addition, it is possible to observe the production of extracellular enzymes, such as proteases and chitinases, in the crude extract of D. flagrans, which besides being necessary for NP production, as mentioned earlier, are able to hydrolyze the cuticle of the nematode, resulting in the death of the parasite. 12,15 Also, these authors suggest that in the future this fungal NP could be used in the control of infective helminth larvae.…”

Section: Introductionmentioning

confidence: 99%

“…D. flagrans is a filamentous nematophagous fungus that has excellent predatory capacity, and is considered the most promising species for the biological control of gastrointestinal parasites. [12][13][14] It produces conidia and chlamydospores that ensure the propagation and survival of the organism under environmental and laboratory conditions. 14 In addition, it is possible to observe the production of extracellular enzymes, such as proteases and chitinases, in the crude extract of D. flagrans, which besides being necessary for NP production, as mentioned earlier, are able to hydrolyze the cuticle of the nematode, resulting in the death of the parasite.…”

Section: Introductionmentioning

confidence: 99%

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Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Barbosa¹,

Silva²,

Ferraz³

et al. 2019

IJN

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Background: Helminth parasites cause morbidity and mortality in both humans and animals. Most anthelmintic drugs used in the treatment of parasitic nematode infections act on target proteins or regulate the electrical activity of neurons and muscles. In this way, it can lead to paralysis, starvation, immune attack, and expulsion of the worm. However, current anthelmintics have some limitations that include a limited spectrum of activity across species and the threat of drug resistance, which highlights the need for new drugs for human and veterinary medicine. Purpose: Present study has been conducted to determine the anthelmintic activity of silver nanoparticles (AgNPs) synthesized from the extract of nematophagous fungus, Duddingtonia flagrans, on the infecting larvae of Ancylostoma caninum (L 3 ). Methods: The nanoparticles were characterized by visual, ultraviolet, Fourier-transform infrared spectroscopy, transmission electron microscopy (TEM) analysis, and X-ray diffraction. The in vitro study was based on experiments to inhibit the motility of infective larvae (L 3 ), and the ultrastructural analysis of the nematode was performed by images obtained by TEM. Results: The XRD studies revealed the crystalline nature of the nanoparticles, and FTIR results implied that AgNPs were successfully synthesized and capped with compounds present in the extract. The results showed that the green synthesis of AgNPs exhibited nematicidal activity, being the only ones capable of penetrating the cuticle of the larvae, causing changes in the tegmentum, and consequently, the death of the nematode. Conclusion: The extract of the fungus D. flagrans is able to synthesize AgNP and these have a nematicidal action.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Barbosa¹,

Silva²,

Ferraz³

et al. 2019

IJN

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“…Also, hyphal density does not determine the trapping ability of nematophagous fungi, as shown in three isolates of Arthrobotrys oligospora (Den Belder and Jansen, 1994). Despite poor growth on the same medium, other researchers found very good activity of other isolates of D. flagrans against different animal parasitic nematode L3 (Araujo et al, 2010;Braga et al, 2013a;Braga et al, 2013b;Campos et al, 2009;Gonzales Garduno et al, 2005;Sanyal, 2000). It is known that different isolates of the same species of fungi show various trapping success (Araújo et al, 1993;Den Belder and Jansen, 1994), including D. flagrans (Larsen et al, 1991).…”

Section: Discussionmentioning

confidence: 95%

Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Simin

Đurić

Kuruca

et al. 2016

Contemporary Agriculture

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Summary Gastro-intestinal nematodes (GIN) of sheep are one of major constraints in grazing production systems worldwide. Control is commonly achieved using anthelmintics, but global occurrence of anthelmintic resistance to different drugs and the emergence of multi-resistant GIN species seriously limit the efficiency of their use. Therefore, integrated parasite management is widely recommended, with nematophagous fungi as one of control tools. Duddingtonia flagrans is one of the most used species, with various effect of different isolates. In previously performed coproculture assay, we showed low efficacy of D. flagrans MUCL 9827 against infective larvae (L3) of sheep GIN. The aim of current experiment was to reevaluate its nematophagous potential, using the medium where direct interaction between the fungus and L3 could be observed. Nematophagous activity was tested on 2% water agar with addition of chloramphenicol on three series of plates seeded with 500 and 1000 chlamidospores and agar blocks with 7 days old mycelium. At Days 0 and 5, 500 L3 of sheep GIN were added to test the trapping activity. The cultures, including control plates with only L3, were incubated at 25°C for 10 days, followed by evaluation of their number and reduction percentage. Nematophagous activity of D. flagrans MUCL 9827 against L3 was clearly demonstrated. However, the overall efficacy was poor since trapping was observed only in one out of nine plates containing fungal material. Potential reasons for such poor performance of the isolate of fungal species, otherwise known as successful in trapping animal parasitic nematodes, are discussed.

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“…Research reports indicate positive results of D. flagrans administration in various formulations for biological control of such parasites as Haemonchus contortus (12,13), Teladorsagia circumcincta (14), Angiostrongylus vasorum (15), Strongyloidae (16) and many more. According to some data, D. flagrans has demonstrated enzyme activity, producing serine proteases (17,18).…”

Section: Introductionmentioning

confidence: 99%

Acute Oral Toxicity of Vetom 21.77 Based on Duddingtonia Flagrans in Broiler Chickens

Rafikova

2019

Macedonian Veterinary Review

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A 14-d study was undertaken to test the acute toxicity of a new preparation Vetom 21.77 based on the predacious fungus Duddingtonia flagrans. A total of 40 healthy 5-day-old broiler chickens (Hubbard F15, 100 ± 5 g), that had previously gone through a required 5-days adaptation to the environment, were orally dosed with the drug for 5 consecutive days at different doses, after which their health status was assessed daily up to the end of the experiment. According to the results, no substantial changes in the physiological state of the chickens were detected during the experiment. Internal organs weighing revealed no statistically significant differences between the groups, though weight coefficient values of internal organs of treated chickens slightly exceeded those of the control group. Some haematological parameters were significantly higher in the treatment group, without going beyond reference ranges. All chickens used in the experiment survived the study. The preparation has not produced any toxic effect even at a higher dose (4000 µL/kg bw/day). It is concluded that Vetom 21.77 pertains to preparations of IV toxicity class.

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In vitro predatory activity of conidia of fungal isolates of the Duddingtonia flagrans on Angiostrongylus vasorum first-stage larvae

Cited by 13 publications

References 14 publications

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

Acute Oral Toxicity of Vetom 21.77 Based on Duddingtonia Flagrans in Broiler Chickens

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