In vitro propagation and assessment of genetic uniformity along with chemical characterization in Hildegardia populifolia (Roxb.) Schott &amp; Endl.: a critically endangered medicinal tree

Upadhyay, Anjali; Shahzad, Anwar; Ahmad, Zuber

doi:10.1007/s11627-020-10085-w

Cited by 17 publications

(6 citation statements)

References 45 publications

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“…It is likely that ex-vitro-established micropropagated plants' increased production of phytochemical substances is attributable to a variety of factors, including the media they are growing in, the explants they are using, their genotype, and even the PRGs they have added to the medium [60,61]. Our findings are in line with previous studies that found that micropropagated plants had more phytochemicals than their donor plants, such as Clerodendrum thomsoniae [62], Hemidesmus indicus [63], Tecoma stans [64], Hildegardia populifolia [65], and Diplocyclos palmatus [61].…”

Section: Discussionsupporting

confidence: 91%

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Qahtan

Faisal

Alatar

et al. 2022

Plants

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Efficient methods for callus induction and the high-frequency plant regeneration of Ruta chalepensis L. were established, and the phytochemical potential and antioxidant activity of a donor plant, ex-vitro-established micropropagated plants, and callus were also studied. Yellowish-green callus was induced with a frequency of 97.8% from internode shoot segments of the donor plant growing in soil in the botanical garden cultured on Murashige and Skoog (MS) medium containing 10 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 1 μM BA (6-benzyladenine). Adventitious shoots were regenerated from the yellowish-green callus on MS medium containing 5.0 μM (BA) and 1.0 μM 1-naphthaleneacetic acid (NAA), with a regeneration frequency of 98.4% and a maximum of 54.6 shoots with an average length of 4.5 cm after 8 weeks. The regenerated shoots were rooted in a medium containing 1.0 μM IBA (indole-3-butyric acid) and successfully transferred to ex vitro conditions in pots containing normal garden soil, with a 95% survival rate. The amounts of alkaloids, phenolics, flavonoids, tannins, and antioxidant activity of the ex-vitro-established micropropagated plants were higher than in the donor plant and callus. The highest contents of hesperidin and rutin (93.3 and 55.9 µg/mg, respectively) were found in the ex-vitro-established micropropagated plants compared to those obtained from the donor plant (91.4 and 31.0 µg/mg, respectively) and callus (59.1 and 21.6 µg/mg, respectively). The genetic uniformity of the ex-vitro-established micropropagated plants was appraised by the ISSR markers and compared with the donor plant. This is the first report describing the callus-mediated plant regeneration, as well as the production of phenolic compounds and antioxidant activities in R. chalepensis, which might be a potential alternative technique for the mass propagation and synthesis of bioactive compounds such as hesperidin and rutin.

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Section: Discussionsupporting

confidence: 91%

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Qahtan

Faisal

Alatar

et al. 2022

Plants

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“…The suitability of the MS medium over WPM and B5 has also been reported by other workers, such as in Magnolia sirindhorniae (Cui et al, 2019 ), Aspilia africana (Okello et al, 2021 ), and Azadirachta indica (Bello et al, 2022 ). In contrast to our study, WPM was found as a suitable growth medium for woody tree species such as Achyrocline satureioides (Guariniello et al, 2018 ), Quercus aliena (Li et al, 2019 ), Hildegardia populifolia (Upadhyay et al, 2020 ), and Alangium salviifolium (Pandey et al, 2022 ).…”

Section: Discussioncontrasting

confidence: 79%

“…The effect of auxin NAA for better rooting was also shown in various studies, such as in Sapindus trifoliathus (Asthana et al, 2011) and A. africana (Okello et al, 2021). Unlike our study, the half-strength MS medium supplemented with IBA was found to be the optimal root growth medium in Rhododendron wattii (Mao et al, 2018), H. populifolia (Upadhyay et al, 2020), Trichilia pallida (de Souza Prim et al, 2021), and Eremurus spectabilis (Basiri et al, 2022).…”

Section: Discussionsupporting

confidence: 72%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

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Casuarina equisetifolia is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic in vitro propagation protocol in C. equisetifolia using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, in vitro derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old in vitro derived plant of C. equisetifolia for synthetic seed production, and it was reported that CaCl2 · 2H2O (100 mM) + Na2-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of C. equisetifolia. It will also help in meeting the growing demands of C. equisetifolia in the forest industry.

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“…For the screening of metabolites in various medicinal plants, a combination of chromatography-mass spectrometry has been frequently utilized. Indeed, a similar approach was used for other medicinal plant species such as Cassia angustifolia [67], Decalepis arayalpathra [68], Zhumeria majdae [69], Hemidesmus indicus [70] and Hildegardia populifolia [71].…”

Section: Gc-ms Analysismentioning

confidence: 99%

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

et al. 2021

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Diplocyclos palmatus (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in D. palmatus.

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In vitro propagation and assessment of genetic uniformity along with chemical characterization in Hildegardia populifolia (Roxb.) Schott & Endl.: a critically endangered medicinal tree

Cited by 17 publications

References 45 publications

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

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