In vitro propagation, clonal fidelity and phytochemical analysis of Rhodiola imbricata Edgew: a rare trans-Himalayan medicinal plant

Bhardwaj, Ashwani; Singh, Baldev; Kaur, Kulwinder; Roshan, Poonam; Sharma, Anu; Dolker, Dechen; Naryal, Avilekh; Saxena, Shweta; Pati, Pratap Kumar; Chaurasia, O. P.

doi:10.1007/s11240-018-1482-x

Cited by 36 publications

(8 citation statements)

References 48 publications

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“…Furthermore, it was seen that the total phenolic and total flavonoid content values slightly increased in micropropagated plants [ 65 ]. These findings are in accordance with the results of Behera et al [ 66 ] and Bhardwaj et al [ 67 ] on Hedychium coronarium and Rhodiola imbricate respectively.…”

Section: Discussionsupporting

confidence: 93%

Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR

Ioannidis¹,

Dadiotis

Mitsis³

et al. 2020

Molecules

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High cannabidiol (CBD) and cannabigerol (CBG) varieties of Cannabis sativa L., a species with medicinal properties, were regenerated in vitro. Explants of nodal segments including healthy axillary bud, after sterilization, were placed in Murashige-Skoog (MS) culture medium. The shoots formed after 30 days were subcultured in full- or half-strength MS medium supplemented with several concentrations of 6-benzyl-amino-purine (BA) or thidiazuron (TDZ). The highest average number and length of shoots was achieved when both full and half-strength MS media were supplemented with 4.0 μM BA. The presence of 4.0 μM TDZ showed also comparable results. BA and TDZ at concentrations of 4.0, 8.0 μM and 2.0, 4.0 μM respectively, displayed the maximum shooting frequency. The new shoots were transferred on the same media and were either self-rooted or after being enhanced with different concentrations of indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA). Presence of 2.0 or 4.0 μM IBA or 4.0 μM NAA resulted to the optimum rooting rates. The maximum average number and length of roots per shoot was observed when the culture media was supplemented with 4.0 μM IBA or NAA. Approximately 92% of the plantlets were successfully established and acclimatized in field. The consistency of the chemical profile of the acclimatized in vitro propagated clones was assessed using quantitative 1H-NMR high throughput screening. In each variety, analysis of the micropropagated plant in comparison with the mother plant showed no statistically significant differences (p ≤ 0.05) in CBD+ cannabidiolic acid (CBDA) and CBG+ cannabigerolic acid (CBGA) content respectively, thus indicating stability of their chemical profile.

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Section: Discussionsupporting

confidence: 93%

Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR

Ioannidis¹,

Dadiotis

Mitsis³

et al. 2020

Molecules

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“…The free radical scavenging activity of the samples (as described preparation of sample for determination of valerenic acid derivatives) were analyzed by 2, 2diphenyl-1-picrylhydrazyl (DPPH, Sigma) method (Singh et al 2016;Bhardwaj et al 2018). 0.2 mL methanolic extracts were mixed with 3 mL of 100 µM DPPH solution and incubated at room temperature (25 ± 2°C) for 30 min under dark condition (A E ).…”

Section: Analysis Of 2 2-diphenyl-1-picrylhydrazyl (Dpph) Activitymentioning

confidence: 99%

Induction and submerged cultivation of Valeriana jatamansi adventitious root cultures for production of valerenic acids and its derivatives

Gehlot

Chaudhary

Devi

et al. 2021

Plant Cell Tiss Organ Cult

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In vitro adventitious roots were induced from Valeriana jatamansi to assess their quality as an alternative ingredient for extraction of secondary metabolites to meet the demand of phytopharmaceutical industries. A signi cantly (p ≤ 0.05) high root induction (90 %) was achieved on Schenk and Hildebrandt medium forti ed with 9.84 µM indole-3-butyric acid. A maximum root biomass (144.09 ± 11.36 g/L) with high relative growth rate (2.01 ± 0.04) and growth index (13.41) was achieved in submerged cultivation. The total valerenic acid derivatives (1525.14 µg/g DW) and acetoxy valerenic acid (534.91µg/g DW) were signi cantly high in induced adventitious roots, with notable quantity of hydroxyl valerenic acid (919.57 µg/g DW) that otherwise not quanti able in parent plant parts. In addition, 0.059% essential oil yield consisting of 24.00% patchouli alcohol was also obtained from induced adventitious roots. The phenolic acid derivatives were also signi cantly higher in adventitious roots (451.58 µg/g DW) as compared to rhizome (187.79 µg/g DW) and leaves (263.68 µg/g DW) of the parent plant. Notably, a substantial increase in phytochemicals was evident at subsequent culture stages with signi cantly reduced in vitro cultivation cycle (2 months) as compared to eld grown plants (24 months). Conclusively, a comparable metabolic pro le of in vitro induced V. jatamansi adventitious roots and considerably shorter growth cycle clearly determines its potential as a feasible source of phytoconstituents.

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“…In contrast, we have established a more efficient protocol forming 12-13 shoots per nodal explants on both solid and liquid media. However, reduced rate of shoot proliferation at 15μM of KN may be due to its weak potential to induce shoot multiplication [49,50]. One of the major challenges involved in the scale-up of in vitro micropropagation method is the cost involved in the process.…”

Section: Concentration Of Pgrs (μM)mentioning

confidence: 99%

“…Auxins are well known as rooting hormones and are routinely used for initiating root development of in vitro shoots [50,53]. It positively regulates the various carbohydrate, nitrogen, protein and polyphenolic metabolic pathways which are well known to influence an array of pathways involved in root growth and development [54].…”

Section: Plos Onementioning

confidence: 99%

Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use

et al. 2020

Self Cite

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Neuroinflammation is a major risk factor associated with the pathogenesis of neurodegenerative diseases. Conventional non-steroidal anti-inflammatory drugs are prescribed but their long term use is associated with adverse effects. Thus, herbal based medicines are attracting major attraction worldwide as potential therapeutic candidates. Tylophora indica (Burm. f) Merrill is a valuable medicinal plant well known in Ayurvedic practices for its immunomodulatory, anti-oxidant, anti-asthmatic and antirheumatic activities. The present study aimed to elucidate the antineuroinflammatory potential of water and hydroalcoholic leaf extracts of micropropagated plants of T. indica using BV-2 microglia activated with lipopolysaccharide as an in vitro model system and development of an efficient reproducible protocol for its in vitro cloning. Non cytotoxic doses of the water and hydroalcoholic extracts (0.2μg/ml and 20μg/ml, respectively) were selected using MTT assay. α-Tubulin, Iba-1 and inflammatory cascade proteins like NFκB, AP1 expression was studied using immunostaining to ascertain the anti-neuroinflammatory potential of these extracts. Further, anti-migratory activity was also analyzed by Wound Scratch Assay. Both extracts effectively attenuated lipopolysaccharide induced microglial activation, migration and the production of nitrite via regulation of the expression of NFκB and AP1 as the possible underlying target molecules. An efficient and reproducible protocol for in vitro cloning of T. indica through multiple shoot proliferation from nodal segments was established on both solid and liquid Murashige and Skoog's (MS) media supplemented with 15μM and 10μM of Benzyl Amino Purine respectively. Regenerated shoots were rooted on both solid and liquid MS media supplemented with Indole-3-butyric acid (5-15μM) and the rooted plantlets were successfully acclimatized and transferred to open field conditions showing 90% survivability. The present study suggests that T. indica may prove to be a potential anti-neuroinflammatory agent and may be further explored as a potential therapeutic candidate for the management of neurodegenerative diseases. Further, the current study will expedite the conservation of T. indica ensuring ample supply of this threatened medicinal plant to fulfill its increasing demand in herbal industry.

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In vitro propagation, clonal fidelity and phytochemical analysis of Rhodiola imbricata Edgew: a rare trans-Himalayan medicinal plant

Cited by 36 publications

References 48 publications

Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR

Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR

Induction and submerged cultivation of Valeriana jatamansi adventitious root cultures for production of valerenic acids and its derivatives

Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use

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