In vitro propagation ofLens species and their F1 interspecific hybrids

Ahmad, Musharaf; Fautrier, A. G.; McNeill, D. L.; Hill, G. D.; Burritt, David J.

doi:10.1007/bf02318954

Cited by 28 publications

(18 citation statements)

References 15 publications

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“…The rooting percentage in this study was higher than in all the previous studies except that of Ahmad et al (1997). One reason may be the lower concentration of external cytokinins used.…”

Section: Discussioncontrasting

confidence: 79%

“…The effectiveness of BA on shoot induction in lentil tissue culture has been well documented (Saxena & King 1987;Polanco et al 1988;Malick & Rashid 1989;Warkentin & McHughen 1993;Ahmad et al 1997;Hallbach et al 1998). Malik & Saxena (1992) reported that TDZ induced multiple shoots directly from intact seedlings of L. culinaris, Pisum sativum, Cicer arietinum, and Phaseolus vulgaris.…”

Section: Discussionmentioning

confidence: 98%

“…Halbach et al (1998) reported that the best shoot formation was from bisected embryos, and that keeping the cotyledons attached to the embryo-axis was beneficial. Ahmad et al (1997) developed a protocol for the micropropagation of lentil and hybrids based on single node culture. Ye et al (2000) further improved the single node culture protocol.…”

Section: Introductionmentioning

confidence: 99%

“…However, the low success rate of artificial crossing has greatly hampered this process. Embryo culture has been used to rescue interspecific hybrids between L. culinaris and L. orientalis (Cohen et al 1984;Ladizinsky et al 1985), and in vitro multiplication of F l hybrids has been used as a way of enlarging hybrid populations (Ahmad et al 1997;Ye et al 2000). However, a promising option to transfer useful genes by genetic transformation has not yet been successful.…”

Section: Introductionmentioning

confidence: 99%

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Multiple shoot formation in lentil (Lens culinaris) seeds

McNeil

Conner

et al. 2002

New Zealand Journal of Crop and Horticultural Science

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A protocol based on seed culture was developed for efficient in vitro propagation of lentil (Lens culinaris Medik). Benzyladenine (BA), thidiazuron (TDZ), and kinetin all induced multiple shoot formation. In terms of the number of long shoots (>2.0 cm) produced per seed, BA and TDZ at optimum concentrations (0.2-0.4 and 0.1 mg/litre, respectively) had similar efficiency, whereas kinetin produced less shoots. Murashige and Skoog (MS) salt composition was better than that of Gamborge (B5) for shoot induction. Increasing calcium (Ca) concentration was necessary to overcome shoot-tip necrosis. For shoot elongation, fresh medium of the same composition of shoot induction medium could be used for stumps from medium with low BA (<0.8 mg/litre) or TDZ (<0.4 mg/litre). Otherwise, MS medium supplemented with 0.5 mg/ litre gibberellic acid (GA 3 ) or 0.05 mg/litre α-naphthaleneacetic acid (NAA) was appropriate. H00045 Received 30 October 2000; accepted 8 November 2001Multiple shoots were induced from 10 inbred of four species. Best rooting was achieved using MS medium supplemented with 1.5 mg/litre NAA. Using this rooting medium more than 50% shoots from the 10 inbreds rooted. From 80 to 100% plantlets of the 10 inbreds survived after being transferred to potting mixture in glass house.

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“…The rooting percentage in this study was higher than in all the previous studies except that of Ahmad et al (1997). One reason may be the lower concentration of external cytokinins used.…”

Section: Discussioncontrasting

confidence: 79%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple shoot formation in lentil (Lens culinaris) seeds

McNeil

Conner

et al. 2002

New Zealand Journal of Crop and Horticultural Science

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“…was optimized on Murashige Skoog (MS) media (Murashige & Skoog 1962) to regenerate shoots in vitro from nodal segments. The number of shoots per explant, the number of nodes per shoot and shoot length was most affected by the concentration of GA 3 and 6-benzyladenine (BA), with only small interaction effects between them (Ahmad et al 1997). Shoots of Acacia mangium were elongated on MS medium containing 0.045 μM thidiazuron (TDZ) supplemented with 7.22 μM GA 3 (Deyu & Hong 2001).…”

Section: Introductionmentioning

confidence: 99%

Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins

et al. 2010

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Lentil

Öktem

Akçay

Bayraç

et al. 2008

Compendium of Transgenic Crop Plants

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Lentils are annual pulse legume plants and their cultivation by humans is as old as the agriculture itself. Although they are very important as a high protein source for the people of various countries in the world and they are vulnerable to a range of biotic and abiotic stresses, they received very little research attention for the improvement of nutritional and growth characteristics. This chapter summarizes various genetic transformation studies and plant regeneration methods used for the propagation and genetic improvement of this important crop plant.

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In vitro propagation ofLens species and their F1 interspecific hybrids

Cited by 28 publications

References 15 publications

Multiple shoot formation in lentil (Lens culinaris) seeds

Multiple shoot formation in lentil (Lens culinaris) seeds

Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins

Lentil

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