Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier &amp; W.E.Higgins

Coello, Christian YanellY; Miceli, Clara Luz; orantes, Carolina; Dendooven, Luc; Gutiérrez, Federico Antonio

doi:10.4067/s0717-66432010000100003

Cited by 16 publications

(13 citation statements)

References 16 publications

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“…Nonetheless, Pedroza (2009), found that the addition of AIA (0.5 mg.L -1 ) to the MS culture medium, promoted the development phases of Epidendrum elongatum. In the same way, Coello et al (2010), highlights the importance of the GA 3 hormone in orchids germination and growth. However, it should be noted that despite the difficulty in analyzing its composition and the lack of information in the literature about the effects of organic components, such as coconut water and pineapple juice, these are rich in energy, vitamins, amino acids and phytohormones.…”

Section: Discussionmentioning

confidence: 99%

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Mercado

Contreras

2017

Acta Agron.

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Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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Section: Discussionmentioning

confidence: 99%

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Mercado

Contreras

2017

Acta Agron.

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“…Cytokinins are known to stimulate plant cell division and participate in the release of lateral bud dormancy, induction of adventitious bud formation and in growth of lateral buds. 10 Different concentrations (0.5 and 1.0 mg dm -3 ) of cytokinins namely 6-benzylaminopurine (BAP) and kinetin (Kn) were used for the shoot regeneration (caulogenesis). Shoot regeneration response was expressed as number of regenerated shoots per explant and plant height (Figure 1 (a-c), 2a).…”

Section: Resultsmentioning

confidence: 99%

Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Sahu

Dewanjee

2012

Nat. Prod. Bioprospect.

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Abstract:Solenostemon scutellarioides is a commercially important ornamental plant. In present study, it was attempted to establish a protocol for high frequency in vitro regeneration of S. scutellarioides. Caulogenesis was found to be significant in solid MS medium supplemented with 0.5 mg dm -3 BAP. Combination of GA 3 (0.5 mg dm -3 ) and BAP (0.5 mg dm -3 ) induced high frequency regeneration coupled with higher plant height. The plantlets exposed to IBA (1 mg dm -3 ) exhibited significant root development in terms of first appearance of root and number of roots per shoot. To improve its commercial acceptability in terms of plant architecture and foliage colouration, two weeks old in vitro grown plantlets were exposed to different culture conditions namely MS strength, sucrose concentration, pH and light. A variable plant types with diverse ornamental traits were developed under different culture conditions.

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“…A los 45 días de iniciado el cultivo, los brotes crecidos en el medio MS al 50% con AG3 (MS50-2) mostraron la mayor longitud, pero al final de la evaluación este medio y el que contenía las sales MS al 100% sin AG3 no fueron estadísticamente diferentes (Cuadro 5). Al respecto, Coello et al (2010) Labrousse et al (2012) evaluated Sorbarod (buffer cellulose), peat and paper pulp as alternative supports to agar in micropropagation for Nemesia denticulata (Benth.) Grant ex Fourc., they found that substrates promoted better root development and increased acclimation survival.…”

Section: Efecto De Medio De Cultivounclassified

“…At 45 days after starting the cultivation the shoots grown on MS medium with 50% AG3 (MS50-2) showed the greatest length, but at the end of the evaluation this medium and the one containing MS salts at 100% without AG3 showed no statistically differences (Table 5). In this regard, Coello et al (2010) found that gibberellic acid was the factor that promoted shoot elongation of Guarianthe skinneri Bateman. On the other hand, the number of leaves was not significantly different between the different treatments throughout the crop.…”

Section: Effect Of Culture Mediummentioning

confidence: 99%

Medio de cultivo y sustitutos del agar en el crecimiento in vitro de orquídeas

Hernández

Robledo-Paz

Jimarez-Montiel

2017

Remexca

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El cultivo in vitro es una técnica que ha permitido la propagación de distintas especies de orquídeas, pero el lento crecimiento de este grupo de plantas, así como el costo de las sales minerales y del agente gelificante (agar) empleados en los medios de cultivo, pueden limitar su aplicación. El objetivo del presente trabajo fue conocer el efecto de la composición del medio de cultivo y el uso de sustratos como sustitutos del agar en el crecimiento in vitro de Laelia anceps Lindl. y Epidendrum sp. Brotes adventicios de ambas especies se cultivaron in vitro en distintas mezclas de sustratos (perlita, tezontle y fibra de coco) y agar con diferentes concentraciones de las sales basales de Murashige y Skoog (50 y 100%) y ácido giberélico (AG3) (0 y 1 mg L-1) para evaluar su longitud, número de hojas, número de raíces y peso fresco en un diseño experimental con tres factores y 15 repeticiones, cada repetición consistio en un frasco con 5 brotes adventicios. El comportamiento in vitro estuvo en función del genotipo; L. anceps Lindl. respondió mejor que Epidendrum sp. Los brotes crecidos sobre perlita-tezontle o fibra de coco-tezontle, y medios con sales al 50% y AG3 no mostraron diferencias significativas en cuanto a su longitud, número de hojas, número de raíces y peso fresco, con respecto a aquellos que lo hicieron en agar, sales al 100% y sin AG3. Los medios de cultivo con sales diluidas sin AG3 y sustratos como sustitutos del agar, permitieron el crecimiento in vitro de las plantas de L. anceps Lindl. y Epidendrum sp. con una reducción significativa del 60 % de los costos.

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Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins

Cited by 16 publications

References 16 publications

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Medio de cultivo y sustitutos del agar en el crecimiento in vitro de orquídeas

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