In Vitro Reaction of Potato Micronodes to Culture Filtrate of Phytophthora Infestans

Crinò, P.; Peñuela, Rosa Magdalena Uscátegui; Martino, L.; Sonnino, A.; Ancora, G.

doi:10.1007/978-3-642-73178-5_54

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(2 citation statements)

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“…Our results demonstrate that the fungal filtrate assayed on cultured single node explants enables a discrimination between tolerant (D6sir6e) and resistant (Kondor) cultivars (Table 1). Analogous results have been obtained with this selection method in potato/Phytophthora infestans interaction (Crin6 et al, 1989).…”

Section: Discussionsupporting

confidence: 60%

“…The response was evaluated as percentage of viable shoots of the total cultured nodes. This technique, that enables an early in vitro selec- tion, has already been utilized in potato/Phytophthora infestans interaction (Crin6 et al, 1989). The discrimination between tolerant and resistant genotypes was possible at 12.5% and 25% while the highest concentration (50%) was too drastic.…”

Section: Inoculationmentioning

confidence: 93%

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Somaclonal variation for resistance to Verticillium dahliae in potato (Solanum tuberosum L.) plants regenerated from callus

et al. 1994

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Plant tissue culture is recognized as an important tool to generate useful genetic variability for crop improvement. Regenerated plants from callus induced from stem explants of Solanum tuberosum cv D6sir6e were assessed by in vitro selection, for resistance to Verticillium dahliae. This fungus is the causal agent of Verticillium wilt, a serious vascular wilt disease both in crops and wild species.The rate of in vitro multiplication by single node cuttings was used as a parameter of screening in two selection cycles with different concentrations of V. dahliae filtrate. One resistant clone was selected and then evaluated by inoculation in the growth chamber. Induced damage, and morphological traits (dry weight, leaf area and tuber production) were estimated. The selected clone was comparable to the resistant control, cv Kondor.The results suggest that genetic variation induced in tissue culture could be utilized to generate disease resistance.

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Section: Discussionsupporting

confidence: 60%

Section: Inoculationmentioning

confidence: 93%