In vitro reaction to orthopaedic biomaterials by macrophages and lymphocytes isolated from patients undergoing revision surgery

Trindade, Michael C. D.; Lind, Martin; Sun, Doo Hoon; Schurman, David J.; Goodman, Stuart B.; Smith, Robert L.

doi:10.1016/s0142-9612(00)00181-2

Cited by 76 publications

(55 citation statements)

References 48 publications

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“…For example, TNF-α was expressed at 12 h for N, LM and LN groups and the protein production did not occur at 12 h or even after 24 h. Another finding was that IL-6 had the highest-fold increase and IL1-β produced more proteins. Other studies in the orthopedic literature described similar results to those of present study (11,15,22), but the reasons for this are unknown. A possible explanation may be that this discrepancy in mRNA expression and protein production is related to post-transcriptional processing or autocrine mechanisms to prevent an over-inflammatory reaction that can be harmful for the system (15).…”

Section: Pro-inflammatory Analysis Of Macrophagessupporting

confidence: 85%

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey's test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis.

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Section: Pro-inflammatory Analysis Of Macrophagessupporting

confidence: 85%

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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“…[140,141] In lymphocyte/macrophage co-cultures, lymphocytes have been observed to associate with macrophages and foreign body giant cells. [142,143] To determine the role of lymphocytes in the foreign body reaction to synthetic biomaterials, we have utilized human blood-derived lymphocytes and monocytes in co-cultures. The lymphocytes and monocytes are cultured directly as well as separated by a membrane via a transwell insert when exposed to biomaterial surfaces in order to investigate the interactions between lymphocytes and macrophages during the foreign body reaction.…”

Section: Lymphocyte/macrophage Interactionsmentioning

confidence: 99%

Foreign body reaction to biomaterials

Anderson

Rodriguez

Chang

2008

Seminars in Immunology

4,246

4,024

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The foreign body reaction composed of macrophages and foreign body giant cells is the end-stage response of the inflammatory and wound healing responses following implantation of a medical device, prosthesis, or biomaterial. A brief, focused overview of events leading to the foreign body reaction is presented. The major focus of this review is on factors that modulate the interaction of macrophages and foreign body giant cells on synthetic surfaces where the chemical, physical, and morphological characteristics of the synthetic surface are considered to play a role in modulating cellular events. These events in the foreign body reaction include protein adsorption, monocyte/ macrophage adhesion, macrophage fusion to form foreign body giant cells, consequences of the foreign body response on biomaterials, and cross-talk between macrophages

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“…Device-related immune reactions have been investigated in mammary [20], vascular [21]and orthopaedic [22]prostheses, on peripheral blood samples from recipient patients, by flow cytometry or on biopsy specimens taken around the implant. These procedures cannot be applied for CL studies, since CLs are not in a direct contact with peripheral blood and, in addition, the relatively traumatic conjunctival biopsy is mainly carried out in patients where a severe pathology is suspected.…”

Section: Discussionmentioning

confidence: 99%

Mononuclear Cell Antigen Expression after Contact with One-Day Disposable Contact Lens Materials

Versura

Iannelli²,

Stefoni³

et al. 2003

Ophthalmologica

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Purpose: To analyse the effects of 1-day disposable contact lens (CL) material on mononuclear cell activation antigens and adhesion molecules, in an in vitro model set up in order to simulate the first 2 h of wear. Methods: Etafilcon A and Hilafilcon A materials were analysed. Mononuclear cells, collected from peripheral blood samples of normal human donors, were placed in contact with the materials, both new and coated with artificial tear solution. As a control, Thermanox coverslips and a group with no material were included. Immunofluorescence reactions were carried out on cells, to study the distribution of activation (HLA-DR, CD25, CD69) and adhesion (CD11a, CD18, CD54) molecules; the flow cytometry technique was used to analyse cells. Results: Statistically significant results were demonstrated for the CD69 and all the 3 adhesion molecules. Artificial tear coating enhanced the mononuclear cell response. Conclusions: The daily mononuclear cell activation can lead, with time, to production of inflammatory mediators that may be responsible for discomfort or, at worst, intolerance and definitely to CL wear remittance.

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In vitro reaction to orthopaedic biomaterials by macrophages and lymphocytes isolated from patients undergoing revision surgery

Cited by 76 publications

References 48 publications

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Foreign body reaction to biomaterials

Mononuclear Cell Antigen Expression after Contact with One-Day Disposable Contact Lens Materials

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