In Vitro Regeneration of Dendrobium sp. of Orchid Using Leaf Tip as Explant

Abstract

Plant growth regulators (PGRs) namely, 2,4-D, NAA and BAP were added into Murashige and Skoog (MS) medium to observe the effect of PGRs on the growth and development of Dendrobium sp. orchid. Leaf tips of Dendrobium sp. were used as explants and inoculated on MS medium supplemented with 2, 4 D (0, 0.5, 2.5, 5, 10 mgL −1 ) for development of PLBs. The maximum PLBs formation (90%) and the maximum number of PLBs (16.00) were observed in 10 mgL −1 2, 4-D into MS medium after 60 days of culture. Subcultured PLBs were inoculated on MS medium supplemented with different combinations of NAA (0, 0.5, 2.5, 5 mgL −1 ) and BAP (0, 0.5, 2.5, 5 mgL −1 ) for shoot regeneration. The maximum number of shoot (11.00), the highest fresh weight (0.6233g) and the highest shoot length (3.613 cm) were observed in 0.5 mgL −1 NAA + 0.5 mgL −1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum plantlet regeneration percentage (93.33%) were observed with the combined effect of 0.5 mg NAA and 0.5 mg BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.

Characterization and Potential of Coelogyne rochussenii Orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary as Explant Source

“…This is because there are thousands of cells capable of differentiating to realize cell totipotency in young leaves, such as the use of leaves in tissue culture of Aerides multiflora orchids (Bhowmik and Rahman 2020a), Dimorphorchis lowii orchids (Jainol and Gansau 2017), Phalaenopsis cv. Surabaya (Balilashaki et al 2015), orchid Dendrobium sp (Goswami et al 2016), Rhynchostylis retusa (L.) Blume orchid (Islam et al 2015), Dendrobium Sonia orchid (Juntada et al 2015), Phalaenopsis sp. orchid (Zahara 2017).…”

Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

“…Thailand is renowned for exporting orchids, both as cut flowers and potted plants [3]. Several species of orchids are cultivated because of their benefits in the medical field and for cultural purposes [4]. The annual export value of orchid flowers and seedlings is over one billion Thai Baht.…”

“…Numbers of orchids are decreasing while demand is increasing. Plant tissue culture can generate an increase in plant numbers by supplying the necessary nutrients to promote plant growth and development [4].…”

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

“…The following supporting information can be downloaded at: , Table S1: Effective plant growth regulators and other factors for the in vitro culture in ornamentals [ 38 , 110 , 111 , 113 , 114 , 123 , 173 , 192 , 308 , 309 , 310 , 311 , 312 , 313 , 314 , 315 , 316 , 317 , 318 , 319 , 320 , 321 , 322 , 323 , 324 , 325 , 326 , 327 , 328 , 329 , 330 , 331 , 332 , 333 , 334 , 335 , 336 , 337 , 338 , 339 , 340 , 341 , 342 , 343 , 344 , 345 , 346 , 347 , 348 , 349 , 350 , 351 , 352 ...…”