In Vitro Sensitivity of the Three Mammalian Collagenases to Tetracycline Inhibition: Relationship to Bone and Cartilage Degradation

Minocycline is More Potent Than Tetracycline and Doxycycline in Inhibiting MMP-9 in Vitro

“…Tetracyclines dose-dependently inhibited MMP-9, and IC50 values of 40.0, 10.7, and 608.0 µM were achieved for tetracycline, minocycline, and doxycycline, respectively. In previous studies on different MMPs using alternative experimental models, IC50 values ranging from 5 to 500 µM have been generally reported (13,(23)(24)(25)(26). In a study on collagenase extracted from rabbit corneas, the inhibitory effects of tetracyclines on enzyme activity were measured using a chromogen substrate (27).…”

Inhibitors of Clostridium histolyticum colagenase

“…The selected drugs have different structures and pharmacological activities, but all share such inhibiting effect [3][4][5][6][7][8][9]. Six ligands were tested: three oxicans, namely Meloxicam, Tenoxicam and Piroxicam, tetracycline, doxycycline and sodium alendronate.…”

Bioresorbable Collagen Membranes for Guided Bone Regeneration

“…In vivo biodegradation of collagen membranes depends on collagenolytic activity; it has, therefore, been suggested that in addition to increasing the collagen structural stability by cross-linking, inhibition of MMP's may further slow down the collagen degradation . It has been shown that Tetracycline, an antibiotic with anti-collagenolytic properties, inhibits MMPs activity (Golub at al 1997, Greenwald at al 1998. It has also been shown that the use of tetracycline-coated expanded polytetrafluoroethylene (ePTFE) barrier membranes results in additional gain of clinical periodontal attachment, most likely due to its antimicrobial activity (Zarkesh et al 1999).…”