In vitro synergistic anti-oxidant activities of solvent-extracted fractions from Astragalus membranaceus and Glycyrrhiza uralensis

Li, Minghua; Xu, Yan; Yang, Wenjian; Li, Jinkui; Xu, Xiaoqing; Xing, Zhang; Chen, Fangtian; Li, Dapeng

doi:10.1016/j.lwt.2011.02.017

Cited by 23 publications

(17 citation statements)

References 30 publications

(26 reference statements)

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“…The results of the current study were similar to previous studies on tilapia (Shi et al, ), swimming crab, Portunus trituberculatus (Meng, Liu, Li, Gao, & Chen, ), and puffer fish (Cheng et al, ). To date, many studies have confirmed that A. membranaceus and its extracts have significant antioxidant activities (Jia et al, ; X. L. Jiang et al, ; M. Li et al, ). In this study, SOD, CAT, and GSH‐Px activities were significantly higher in the liver of AMEP‐supplemented groups than those in the NC group at 10°C, whereas the MDA content showed a reverse trend.…”

Section: Discussionmentioning

confidence: 96%

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Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Yang

Huang

et al. 2019

J World Aquaculture Soc

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A 54‐day experiment was conducted to investigate the effects of dietary supplementation of Astragalus membranaceus extract powder (AMEP) on the low temperature tolerance of genetically improved farmed tilapia, Oreochromis niloticus. The base diet was supplemented with 0.0% (NC), 0.1% (NP0.1), 0.2% (NP0.2), and 0.4% (NP0.4) AMEP. Tilapia with an initial body weight of 21.35 ± 0.17 g were fed three times a day for 14 days at 30°C and then fed for 40 days while the water temperature decreased at a rate of 0.5°C/day. The results showed that survival, final weight, and the rate of weight gain in fish in the NP0.1, NP0.2, and NP0.4 groups were higher than those of fish in the NC group. The contents of glucose, triglyceride, cholesterol, and nitric oxide in the serum of fish fed diets supplemented with AMEP were all significantly lower than those of fish in the NC group. In contrast, fish fed with AMEP‐supplemented diets had higher liver superoxide dismutase, glutathione peroxidase and catalase activities, and lower malonaldehyde content than those of fish in the NC group at 10°C. The expression of the growth‐arrest‐specific 2 gene in the liver was significantly lower in the NP0.2 and NP0.4 groups than in the NC group. The results of this study demonstrated that the optimal level of AMEP supplementation was 0.2% under the conditions used in the current study. Optimum dietary AMEP can promote growth performance and facilitate the survival of tilapia under low‐temperature stress.

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Section: Discussionmentioning

confidence: 96%

“…To date, many studies have confirmed that A. membranaceus and its extracts have significant antioxidant activities (Jia et al, 2012;X. L. Jiang et al, 2014;M. Li et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Yang

Huang

et al. 2019

J World Aquaculture Soc

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“…in previously published papers, there is little information about flavonoid content of Fabaceae species. They include Glycyrrhiza echinata, G. uralensis and Indigofera species (Bakasso et al, 2008, Cakmak et al, 2012, Li et al, 2011. in G. echinata aerial and root parts, the flavonoid content reached 116.54mgRE/g and 99.64mgRE/g, respectively.…”

Section: Discussionmentioning

confidence: 97%

Capacidad antioxidante y composición de ácidos grasos de diferentes partes de Adenocarpus complicatus (Fabaceae) de Turquía

Berber

Zengin

Şanda

et al. 2014

RBT

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Abstract:Adenocarpus complicatus is distributed throughout the Anatolian peninsula and is widely used for human and animal nutrition. The purpose of this work was to study the antioxidant properties and fatty acid composition of different parts of this plant (fruits and mixed materials). The species was collected from Golyuzu village of the Seydisehir district near Konya province, Turkey. Fruit and mixed parts obtained from this species were ground and a 15g sample was used to prepare methanolic extracts. Powdered plant samples were extracted with 100mL methanol in a mechanical shaker. The obtained extracts were filtered and concentrated to dryness under reduced pressure and were subsequently stored at -20°C. Antioxidant components, namely total phenolic and flavonoid content, were detected for each extract using spectrophotometric methods. Antioxidant capacity was evaluated by various assays including phosphomolybdenum, DPPH free radical scavenging capacity, metal chelating activity, and ferric and cupric ion reducing power. The fatty acid profiles of plant parts were also determined by using gas chromatography. The total phenolic content of fruit (36.21mgGAE/g) was higher than that of mixed materials (13.79mgGAE/g). The methanolic extract of mixed material had higher amounts of flavonoid than fruit extract. The free radical scavenging activity of extracts was expressed as iC 50 value (µg/mL) (amount required to inhibit DPPH radical formation by 50%). The lower iC 50 value reflects better free radical scavenging action. The radical scavenging activity of the samples was compared with BHT, it showed the mixed material to be almost two times more potent than the fruit extract. However, BHT is an excellent free radical scavenger with an iC 50 of 34.061µg/mL. The ferric and cupric reducing power potentials of the extracts were expressed as EC 50 value (the effective concentration at which the absorbance was 0.5). Fruit extract exhibited strong ferric reducing power with an EC 50 of 871.25µg/mL. The metal chelating activity of the extracts increased with concentration. Chelating effect was 83.60% for fruit extract at 1mg/mL concentration. Oil content of fruit and mixed parts were detected as 6.71 and 6.14%, respectively. A total of 32 fatty acids were found in the oil. Essential fatty acids (linoleic and α-linolenic acid) were identified as the most abundant fatty acids in the oil. These results demonstrated that this plant species can be considered as an alternative to synthetic antioxidants. Likewise, the oil obtained from the plant can be used as a source of essential fatty acids for food and pharmacological applications. Rev. Biol. Trop. 62 (1): 337-346. Epub 2014 March 01.

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“…We also observed that the percentage yield of the extract obtained from combining the powders of all nine herbs, in equal weight proportions, was higher than the sum of those obtained from extracting each herb alone. This implied that by combining these herbs, the extraction environment might have been altered due to the induction of complexation or polymerization, resulting in the alteration of the solubility of the constituents 25, 26…”

Section: Discussionmentioning

confidence: 99%

Synergistic antioxidant action of Phikud Navakot ameliorates hydrogen peroxide-induced stress in human endothelial cells

Nalinratana

Kaewprem

Tongumpai

et al. 2014

Integrative Medicine Research

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BackgroundPhikud Navakot (PN), a combination of nine herbs, has been used traditionally in Thai medicinal formulas to relieve circulatory disorder. The present study aimed to compare the synergistic antioxidant efficacy and toxicity of the hydroethanolic and water extracts of PN at cellular level.MethodsPN and its nine herbs were extracted with either 50% ethanol or water. All extracts were tested for in vitro antioxidant potential using standard antioxidant assays. Evaluation of cytotoxicity, genotoxicity, and intracellular reactive oxygen species were performed using human endothelial ECV304 cells.ResultsAntioxidant assays in cell-free systems showed that the hydroethanolic extract of PN scavenged superoxide, hydroxyl, nitric oxide radicals, and hydrogen peroxide more effectively than its water extract. Combination indices were calculated to show that the ingredients of the hydroethanolic extract acted synergistically to exhibit antioxidant activities against all tested radicals, whereas, in the case of water extract, this effect was observed only against 2,2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radicals. A cell-based assay also revealed that the hydroethanolic extract concentration-dependently attenuated hydrogen peroxide-induced stress more effectively than the water extract. At the antioxidant and cytotoxic concentrations of both extracts, no genotoxicity was found.ConclusionOur findings demonstrate that the synergistic antioxidant action of PN ameliorates endothelial stress, which may provide some clues for understanding the traditional use of PN for the treatment of circulatory disorder. Additionally, the selection of a suitable solvent for the extraction of PN herbal combination is essential for maximal efficacy and safety.

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In vitro synergistic anti-oxidant activities of solvent-extracted fractions from Astragalus membranaceus and Glycyrrhiza uralensis

Cited by 23 publications

References 30 publications

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Improving low‐temperature stress tolerance of tilapia, Oreochromis niloticus: A functional analysis of Astragalus membranaceus

Capacidad antioxidante y composición de ácidos grasos de diferentes partes de Adenocarpus complicatus (Fabaceae) de Turquía

Synergistic antioxidant action of Phikud Navakot ameliorates hydrogen peroxide-induced stress in human endothelial cells

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