In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori

Zhang, Lufeng; Zhang, Liang; Deng, Hui; Li, Huan; Tang, Wentao; Guan, Lu; Qiu, Ye; Donovan, Michael; Chen, Zhuo; Tan, Weihong

doi:10.1038/s41467-021-22286-x

Cited by 138 publications

(110 citation statements)

References 47 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“…After three-week infection, the results of urease test, plate coating and hematoxylin-eosin (H&E) staining showed that the H. pylori infected mice were successfully modeled (Figure S18, Supporting Information). [20] Next, the corresponding intragastric administration was carried out, and the mice were sacrificed on the second day after stopping administration for subsequent biochemical experiments. The antibacterial capacity of Pd(H) @ ZIF-8 @ AP in vivo was initially evaluated.…”

Section: In Vivo H Pylori Infection Therapy Of Pd(h) @ Zif-8 @ Apmentioning

confidence: 99%

Metal–Organic‐Framework‐Based Hydrogen‐Release Platform for MultieffectiveHelicobacter PyloriTargeting Therapy and Intestinal Flora Protective Capabilities

et al. 2021

View full text Add to dashboard Cite

urea into ammonia to increase the pH around thallus to resist the destruction of gastric acid, which is also one essential cause for colonizing in the gastric mucosa chronically. [2] The virulence factors generated by H. pylori can directly act on gastric mucosa, which stimulate mucosal cells, neutrophils and macrophages to secrete plenty of inflammatory chemokines, thereby inducting severe mucosal inflammatory reactions. [3] Furthermore, activated inflammatory cells would produce excessive oxygen free radicals through respiration, leading to mitochondrial damage and protein denaturation of gastric mucosal epithelial cells, thereby causing oxidativestress-mediated gastric mucosal disruption, which further resulting in gastric diseases, such as chronic gastritis and peptic ulcer. [4] Nowadays, the antibiotic therapy dominated by clarithromycin, metronidazole or amoxicillin is used as the first-line treatment for H. pylori in the world. [5] However, for the lack of targeting property on H. pylori, the long-term use of antibiotics tends to destroy the homeostasis of intestinal flora. [6] Furthermore, although antibiotics can destroy H. pylori, it is still a formidable challenge of antibiotics to address the dysregulation of inflammation response and the impaired gastric mucosa. Therefore, it is urgent to develop a new antibiotic replacement therapy to achieve the following three requirements: I. Targeting inflammation precisely and eliminating H. pylori effectively. II. Regulating hyperactive immunoreaction and repairing damaged gastric mucosa. III. Protecting intestinal flora.Herein, we have designed a metal-organic framework hydrogen-generation system (Pd(H) @ ZIF-8 @ AP) that is formed by a hydrogen-generation nanoparticle based on a metalorganic framework (Pd(H) @ ZIF-8) and the negatively charged ascorbate palmitate (AP) hydrogel (Scheme 1a), which has three advantages (Scheme 1b): I) Inflammation-targeting and multiple antibacterial properties. It has been reported that longterm colonization of H. pylori leads to chronic gastric mucosal inflammation, [7] accompanied by increased expression of positively charged proteins and matrix metalloproteinase (MMP, IV collagenase), resulting in positive charge in the inflammatory site. [8] Therefore, AP hydrogel with both negative charge Helicobacter pylori (H. pylori) infection is the leading cause of chronic gastritis, peptic ulcer, and gastric cancer. Antibiotics, as traditional method for eliminating H. pylori, have no targeting effect, which causes serious bacterial resistance and gut dysbacteriosis. Moreover, antibiotics can hardly address hyperactive inflammatory response or damaged gastric mucosal barrier caused by H. pylori infection. Here, a pH-responsive metal-organic framework hydrogen-generation nanoparticle (Pd(H) @ ZIF-8) is reported, which is encapsulated with ascorbate palmitate (AP) hydrogel. Both in vitro and in vivo experiments demonstrate that the outer AP hydrogel can target and adhere to the inflammatory site through electrostatic interactions,...

show abstract

Section: In Vivo H Pylori Infection Therapy Of Pd(h) @ Zif-8 @ Apmentioning

confidence: 99%

Metal–Organic‐Framework‐Based Hydrogen‐Release Platform for MultieffectiveHelicobacter PyloriTargeting Therapy and Intestinal Flora Protective Capabilities

et al. 2021

View full text Add to dashboard Cite

show abstract

“…As presented in Supplementary Fig. 24 , we have analyzed the intrinsic oxidase-mimetic activity of these Art Ms, which suggests that the Fe-Art M exhibits very high catalytic transformation of oxygen into ROS via activation and cleavage of the O–O bond 33 . This good contact killing activity of Fe-Art M preliminarily supports the proposed concept for the bacterial “capture and killing” function of hedgehog artificial macrophage.…”

Section: Resultsmentioning

confidence: 99%

Hedgehog artificial macrophage with atomic-catalytic centers to combat Drug-resistant bacteria

Long

et al. 2021

Nat Commun

120

View full text Add to dashboard Cite

Pathogenic drug-resistant bacteria represent a threat to human health, for instance, the methicillin-resistant Staphylococcus aureus (MRSA). There is an ever-growing need to develop non-antibiotic strategies to fight bacteria without triggering drug resistance. Here, we design a hedgehog artificial macrophage with atomic-catalytic centers to combat MRSA by mimicking the “capture and killing” process of macrophages. The experimental studies and theoretical calculations reveal that the synthesized materials can efficiently capture and kill MRSA by the hedgehog topography and substantial generation of •O2− and HClO with its Fe2N6O catalytic centers. The synthesized artificial macrophage exhibits a low minimal inhibition concentration (8 μg/mL Fe-Art M with H2O2 (100 μM)) to combat MRSA and rapidly promote the healing of bacteria-infected wounds on rabbit skin. We suggest that the application of this hedgehog artificial macrophage with “capture and killing” capability and high ROS-catalytic activity will open up a promising pathway to develop antibacterial materials for bionic and non-antibiotic disinfection strategies.

show abstract

“…They have been applied to energy science, electronics, flexible materials, catalytic science, imaging, environmental science, analytical sensing, and biomedicine. [151][152][153][154] As a new class of nanozymes, carbon has been extensively reported in this field. [155] For the graphene nanozymes, the GO-COOH nanozyme have been reported by Qu et al The fabricated GO-COOH nanozyme possess the horseradish PODlike enzymes catalytic.…”

Section: Non-metal-based Nanozymesmentioning

confidence: 99%

Recent Advances in Nanozymes: From Matters to Bioapplications

Liang

et al. 2021

Adv Funct Materials

245

115

View full text Add to dashboard Cite

The manufacture of bionic materials to simulate the natural counterparts has attracted extensive attention. As one of the subcategories of biomimetic materials, the development of artificial enzyme is intensive pursuing. As a kind of artificial enzyme, nanozymes are dedicated to solve the limitations of natural enzymes. In recent years, attributed to the explosive development of nanotechnology, biotechnology, catalysis science, computational design and theory calculation, research on nanozymes has made great progress. To highlight these achievements and help researchers to understand the current investigation status of nanozyme, the state‐of‐the‐art development in nanozymes from fabrication materials to bioapplications are summarized. First different raw materials are summarized, including metal‐based, metal‐free, metal‐organic frameworks‐based, and some other novel matters, which are applied to fabricate nanozymes. The different types of enzymes‐like catalytic activities of nanozymes are briefly discussed. Subsequently, the wide applications of nanozymes such as anti‐oxidation, curing diseases, anti‐bacteria, biosensing, and bioimaging are discussed. Finally, the current challenges faced by nanozymes are outlined and the future directions for advancing nanozyme research are outlooked. The authors hope this review can inspire research in the fields of nanotechnology, chemistry, biology, materials science, and theoretical computing, and can contribute to the development of nanozymes.

show abstract

In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori

Cited by 138 publications

References 47 publications

Metal–Organic‐Framework‐Based Hydrogen‐Release Platform for MultieffectiveHelicobacter PyloriTargeting Therapy and Intestinal Flora Protective Capabilities

Metal–Organic‐Framework‐Based Hydrogen‐Release Platform for MultieffectiveHelicobacter PyloriTargeting Therapy and Intestinal Flora Protective Capabilities

Hedgehog artificial macrophage with atomic-catalytic centers to combat Drug-resistant bacteria

Recent Advances in Nanozymes: From Matters to Bioapplications

Contact Info

Product

Resources

About