In vivo and in vitro visualization of gene expression dynamics over extensive areas of the brain

Eguchi, Megumi; Yamaguchi, Shun

doi:10.1016/j.neuroimage.2008.10.046

Cited by 102 publications

(117 citation statements)

References 32 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…The immunohistochemical and Western blot analyses using the anti-Arc and anti-luciferase antibodies supported spatiotemporal expression patterns of the bioluminescence signals accurately reflecting the endogenous Arc and transgenic Luc protein expression patterns. Recently, three research groups have reported the successful in vivo imaging of Arc expression by the knockin of d2EGFP into the Arc locus (Wang et al 2006), the conventional transgenic method using the 7.1-kbp 5 0 upstream region of Arc and the fluorescent protein reporter Venus (Eguchi and Yamaguchi 2009), and the BAC transgenic method using the short-life form d4EGFP (Grinevich et al 2009). The reported expression patterns of Arc-dVenus in the visual cortex of the transgenic mice were similar, as reported here and also in other reports (Kawashima et al 2009;Tagawa et al 2005).…”

Section: Strengths and Limitations Of Our Arc-luc Tg Mouse Strainmentioning

confidence: 99%

“…They are the d2-type of enhanced green fluorescent protein (EGFP) gene (d2EGFP) knocked into the Arc locus in mice (Wang et al 2006), the fluorescent protein reporter gene Venus linked to the 7.1 kbp promoter region of Arc in conventional transgenic (Tg) mice (Eguchi and Yamaguchi 2009), and the short-life form of EGFP, d4EGFP, introduced into the bacterial artificial chromosome (BAC) containing Arc in BAC Tg mice (Grinevich et al 2009). Wang et al (2006) successfully monitored Arc expression at a single neuron level from cortical layers II to IV during visual cortical activation using visual stimuli.…”

Section: Introductionmentioning

confidence: 99%

“…Wang et al (2006) successfully monitored Arc expression at a single neuron level from cortical layers II to IV during visual cortical activation using visual stimuli. The latter two research groups obtained superficial brain fluorescence signals of Venus and d4EGFP from the visual cortex and somatosensory cortex (Eguchi and Yamaguchi 2009;Grinevich et al 2009) with some autofluorescence noise. These groups could not detect signals of Arc expression from deep brain regions such as the hippocampus, and changes in signals associated with experience-dependent plasticity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

et al. 2010

View full text Add to dashboard Cite

Induction of the activity-regulated cytoskeleton-associated protein gene (Arc), one of the immediate early genes, in the brain correlates with various sensory processes, natural behaviors, and pathological conditions. Arc is also involved in synaptic plasticity during development. Thus, in vivo monitoring of Arc expression is useful for the analysis of physiological and pathological conditions in the brain. Recently, in vivo imaging of Arc expression using various green fluorescent protein-based probes has been reported; however, these probes can only be applied for the detection of fluorescence signals from superficial layers of the cortex with some autofluorescence noise. Here, we generated a novel transgenic mouse strain to monitor the neuronal-activity-dependent Arc expression using bioluminescence signals in vivo. Because of the very high sensitivity with a high signal-to-noise ratio, we detected neuronal-activity-dependent plastic changes in the bioluminescence signal intensity in the mouse visual cortex after visual deprivation, suggesting structural plasticity after peripheral lesions in adults. We also detected drastic changes in bioluminescence signals after seizure induction with kainic acid. Our novel mouse strain will be valuable for the continuous monitoring of neuronal-activity-dependent Arc expression in the brain under physiological and pathological conditions.

show abstract

Section: Strengths and Limitations Of Our Arc-luc Tg Mouse Strainmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The ability to perform in vivo monitoring of gene expression dynamics in brain areas with subsequent in vitro assessment is another promising area in animal imaging research (Eguchi and Yamaguchi, 2009). In this case, optical imaging such as a bioluminescent or fluorescent imaging technique can be a major imaging modality, with most studies being designed for small animals such as mice.…”

Section: Statistical Processing: Codification Of Variabilitymentioning

confidence: 99%

Computer‐based morphometry of brain

Koo¹,

Kim²

2010

Int J Imaging Syst Tech

View full text Add to dashboard Cite

Over the past decade, the importance of probing the anatomy of the brain has reemerged as an important field of neuroscience. In combination with functional imaging techniques, the rapid advancement of neuroimaging techniques-such as magnetic resonance imaging-and their growing applicability in studying brain morphometry has led to great advances in neuroscience research. Considering the requirements of the diverse technologies-from image processing to statistics-in performing morphometry of the brain, it is critical to have an overall understanding of this subject. The major objective of this review is to provide a practical introduction to this field. The review starts by covering basic concepts and techniques that are commonly used in morphometry of structural magnetic resonance imaging and then extends to further technical perspectives. V

show abstract

“…In transgenic mice, recently activated neurons are labelled by a reporter protein that is expressed under the control of an endogenous promoter of neuronal activity-regulated genes (ie. c-fos, egr-1 or Arc) (Eguchi & Yamaguchi, 2009, Smeyne et al, 1992. After memory reactivation FACS is subsequently used to isolate neurons expressing the reporter protein.…”

Section: Honing In On Memory-related Dna Methylation and Gene Transcrmentioning

confidence: 99%

DNA methylation: an epigenetic watermark of former cocaine self-administration

Baker‐Andresen¹

View full text Add to dashboard Cite

In vivo and in vitro visualization of gene expression dynamics over extensive areas of the brain

Cited by 102 publications

References 32 publications

Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

Computer‐based morphometry of brain

DNA methylation: an epigenetic watermark of former cocaine self-administration

Contact Info

Product

Resources

About