In vivo and in vitro immunomodulatory activities of Trichilia glabra aqueous leaf extracts

Benencia, Fabián; Courrèges, Marı́a C.; Coulombié, Félix C.

doi:10.1016/s0378-8741(99)00010-0

Cited by 40 publications

(13 citation statements)

References 14 publications

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“…Studies with other plant species have been reported, such as Benencia et al (2000), who identified dosedependent immunosuppressive activity in an extract from Trichilia glabra, which displays anti-viral, antiinflammatory and anti rheumatic properties. Sharma et al (1994) detected an immunostimulatory potential in extracts from leaves of Picrorhiza kurroa, used for anti-inflammatory, anti-arthritis, gastrointestinal and urinary disorders.…”

Section: Discussionmentioning

confidence: 99%

“…Eighteen hours later, cells were collected onto filter mats using an automated cell harvester, and the incorporation of tritiated thymidine into the cells was measured by a liquid scintillation counter (Tricarb 2100, USA). The results were expressed as the mean of three determinations minus the background using cells incubated with RPMI 1640-supplemented medium (Alvarez et al, 2004;Benencia et al, 2000;Peijie et al, 2003;Tulinska et al, 2000).…”

Section: Lymphproliferative Responsementioning

confidence: 99%

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Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Feres¹,

Toledo²,

Tagliati³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Avaliação dos efetos imunotoxicológicos de Dimorphandra mollis Benth., Fabaceae, em ratos". A Dimorphandra mollis Benth., Fabaceae, conhecida como faveira ou favad´anta, é uma planta comum do cerrado central do Brasil, muito utilizada por suas propriedades antioxidante, antiplaquetária e, principalmente, como vasoprotetora. Seu principal marcador é a rutina. Este estudo teve como objetivo avaliar a segurança da utilização do extrato seco de D. mollis em roedores. O extrato foi extraído, padronizado e quantificado apresentando teor de 76,0±3% de rutina. Nos parâmetros bioquímicos e hematológicos avaliados, não se observou alterações nos grupos de machos e fêmeas que receberam a dose de 1000 e 2000 mg/kg de D. mollis, mas observou-se um aumento de eosinófilos. Nos estudos histopatológicos detectou-se hiperreatividade da polpa branca esplênica, no grupo que recebeu a dose de 2000 mg/kg de D. mollis. Na avaliação da resposta linfoproliferativa, com 1000 e 2000 mg/kg não foram observadas alterações, e somente nos estudos com a dose de 2000 mg/kg se observou diminuição de IgG. Os resultados obtidos, utilizando roedores, sugerem que nenhuma toxicidade existe na administração de extrato seco de D. mollis na dose de 1000 mg/kg. Unitermos: Dimorphandra mollis, rutina, imunotoxicologia, roedores.ABSTRACT: Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d´anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.

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Section: Discussionmentioning

confidence: 99%

Section: Lymphproliferative Responsementioning

confidence: 99%

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Feres¹,

Toledo²,

Tagliati³

et al. 2010

Rev. bras. farmacogn.

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“…Based on the protocols of Benencia et al (2000), naïve mice were euthanized by cervical dislocation and their spleens were removed. Single cell suspensions were prepared by mincing and tapping spleen fragments on a stainless 200-mesh held in RPMI 1640 medium.…”

Section: Preparation Of Splenocytesmentioning

confidence: 99%

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

Yang

Wan

et al. 2016

Journal of Immunotoxicology

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View Crossmark data Citing articles: 3 View citing articles JOURNAL OF IMMUNOTOXICOLOGY, 2016 VOL. 13, NO. 4, 548-556 http://dx.doi.org/10.3109/1547691X.2016 RESEARCH ARTICLEIn vitro effect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production Gasoline exhaust particles (GEP) and diesel exhaust particles (DEP) are considered to be some of the most important air pollutants. Among the many constituents in these pollutant particles, 4-pentylphenol (PP) and 3-methyl-4-nitrophenol (PNMC) are considered important phenolics in GEP and DEP, respectively. The aim of this study was to investigate the effect of in vitro exposure to commercially-supplied PP and PNMC on populations of, and production of interleukin (IL)-2, IL-4 and granzyme-B by, mouse splenic lymphocytes. After in vitro exposure to PP or PNMC for 48 h, splenocyte viability was measured, cell phenotypes, e.g. B-cell (CD19), T-cells (CD3), T-cell subsets (CD4 and CD8), were quantified by flow cytometry and production of IL-2, IL-4 and granzyme-B was assessed via ELISA. The oxidative toxicity of PP and PNMC toward the splenocytes was also evaluated using measures of hydroxyl radical and malondiadehyde production and changes in glutathione peroxidase and superoxide dismutase activities. Results showed that in vitro exposure to PP and PNMC inhibited splenic cell parameters in a dose-related manner. Exposure to PP and PNMC decreased splenic T-lymphocyte populations and splenocyte production of cytokines and granzyme B, as well as induced oxidative stress in the splenocytes. The results also showed that the percentages of CD3 + T-cells overall and of CD4 + and CD8 + T-cells therein, among exposed splenocytes, were reduced; neither compound appeared to affect levels of CD19 + B-cells. Overall, the suppressive effects of PP were stronger than PNMC. The data here provide support for the proposal that PP-/PNMC-induced toxicity in splenocytes may be due at least in part to oxidative damage and that PP and PNMC -as components of GEP and DEP -might significantly impact on splenic T-cell formation/release of cytokines/granzymes in situ.ARTICLE HISTORY

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“…A avaliação da segurança e eficácia de novas drogas e medicamentos, incluindo fitoterápicos e outros compostos botânicos, deve incluir a realização de ensaios pré-clinicos para avaliação de potenciais efeitos imunomoduladores e imunotóxicos (Putman et al, 2002, World Health Organization, 1998 (Li, 1991;Benencia et al, 2000;Davis e Kuttan, 2000;Plaeger, 2003). Vários testes podem ser utilizados para avaliar a imunomodulação e imunotoxicidade de substâncias ou compostos, como drogas, produtos químicos e extratos de vegetais, brutos ou derivados (Luster et al, 1992 Luster, 1992).…”

Section: A Família Amaranthaceae E O Gênero Alternantheraunclassified

Avaliação da atividade imunomoduladora de 'Alternanthera tenella' Colla e investigação de ações do extrato aquoso em modelo de artrite experimental

Biella¹

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In vivo and in vitro immunomodulatory activities of Trichilia glabra aqueous leaf extracts

Cited by 40 publications

References 14 publications

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

Avaliação da atividade imunomoduladora de 'Alternanthera tenella' Colla e investigação de ações do extrato aquoso em modelo de artrite experimental

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