In Vivo and In Vitro Antibacterial Activities of Cranberry Extract against E. coli O157:H7 in Urinary Tract Infected Rats

Abstract

| The objective of this study was to determine the in-vitro and in-vivo activity of cranberry extracts against Escherichia coli O157:H7. This strain of E. coli was the most common etiologic agent of urinary tract infections isolated from patients. Filter sterilized aqueous and methanol extract of cranberry was prepared and used in the present study. The aqueous extract of cranberry produced inhibition zone ranging from (10.8 -23.8) mm against the tested bacteria. While the methanol extract produces larger zones of inhibition (12.1 -24.2) mm against the bacteria. The minimum inhibitory concentration (MIC) for the methanol and aqueous extract was 0.35 and 0.625 mg/ml, respectively. In vivo study involved inducing UTI in rats and then treated with (200 mg/kg B.W) aqueous and methanol extract and compared with Gentamicin treatment at a dose of (2 mg/kg B.W) subcutaneously for 14 days. Methanol extract succeeded in treated UTI caused by Escherichia coli in the infected rats and prevented infection comparing with aqueous extract and Gentamicin. Food, water intake, body weight, pH and creatinine level returned to normal values after treatment with methanol extract of Cranberry fruit (200mg/Kg. B.W) comparing with aqueous extract of Cranberry fruit and 2mg/Kg. B.W. of Gentamicin. These parameters used in this current study as indicator for curing from infection. These findings indicated that cranberry extract was effective at all levels in inhibiting E. coli O157:H7; thus it possesses antimicrobial activity and hold great promise as an antimicrobial agent. Keywords

Role of Plantaricin in Treatment of UTIs in Female Rats

“…The result of bacterial count was agreement with [51]. Also, that was agreement with [34,35]they demonstrated that within 7 days of Egypt. J. Vet.…”

“…55, No. 6 (2024) causing urinary tract infection in female rats by injecting pathogenic E. coli O157:H7 through inoculating the rats with (2.6 10 6 ) CFU/ml led to effective colonization of E. coli O157H:7 [ 34]. Plantaricin could inhibit the growth of E.coli [29].…”

Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective against Urinary Tract Bacteria

“…To the best of our knowledge, there are no in vivo studies relevant to the antibacterial activity of anthocyanins from blueberries. However, Ibrahim et al [ 93 ] showed that cranberry anthocyanin extracts (200 mg/kg b.w aqueous and methanol extracts) were effective in treating UTI caused by E. coli O157:H7 in infected rats. Although the anthocyanin profiles are not exactly the same in blueberries and cranberries, we might expect a similar behaviour.…”

Detection of Antimicrobial Activity of Cranberry Fruit Extract against Methicillin Resistant Staphylococcus aureus and Extended Spectrum Beta Lactamase Escherichia coli

“…Enterobacteriaceae and Staphylococci has already negatively affected β-lactam-based therapy, so it is assumed that in near future a complete resistance to most effective β-lactam agents is going to surface [18][19][20]. In our study, we have tried to explore the effect of a natural derivative, cranberry fruit extract as it has adjuvant properties of interfering the resistance and can also enhance the efficacy of β-lactam agents.…”

“…The content of flask was filtered through what man and evaporated to dryness in oven at 50°C. After the cranberry fruit powder had been obtained, different concentrations of cranberry fruit extract (20,30,40, 50 and 60 mg/ml) were prepared by mixing the cranberry fruit powder with dimethyl sulfoxide (DMSO) and stored at 4°C in air tight bottles [14].…”