2017
DOI: 10.3389/fpls.2017.01454
|View full text |Cite
|
Sign up to set email alerts
|

In vivo Assembly in Escherichia coli of Transformation Vectors for Plastid Genome Engineering

Abstract: Plastid transformation for the expression of recombinant proteins and entire metabolic pathways has become a promising tool for plant biotechnology. However, large-scale application of this technology has been hindered by some technical bottlenecks, including lack of routine transformation protocols for agronomically important crop plants like rice or maize. Currently, there are no standard or commercial plastid transformation vectors available for the scientific community. Construction of a plastid transforma… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
17
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
6
2
1

Relationship

2
7

Authors

Journals

citations
Cited by 25 publications
(17 citation statements)
references
References 53 publications
0
17
0
Order By: Relevance
“…The plastid transformation vectors used in this study were constructed based on the previously described vector pYY12 (Wu et al ., 2017). The gfp cassette in pYY12 was replaced by the dsRNA expression cassette excised from pJZ197 (Zhang et al ., 2015) by digestion with HincII and NotI, resulting in plasmid pWW1.…”
Section: Methodsmentioning
confidence: 99%
“…The plastid transformation vectors used in this study were constructed based on the previously described vector pYY12 (Wu et al ., 2017). The gfp cassette in pYY12 was replaced by the dsRNA expression cassette excised from pJZ197 (Zhang et al ., 2015) by digestion with HincII and NotI, resulting in plasmid pWW1.…”
Section: Methodsmentioning
confidence: 99%
“…The coding region of the AtGR2 gene (Locus: AT3G54660), omitting a 222-bp sequence encoding chloroplast transit peptide (Kudo et al, 1993), was amplified by using a primer pair AtGR2-Fwd-1 (TTTAAGAAGGAGATATACCCATGAGTACC GATAATGGAGCTGAATC)/AtGR2-Rev-1 (AGCCTTTCGTTTTATTTGATTCTAG ATTCTACACCCCAGCAGCTGTTTTAG). The primers harbor (underlined) a short homologous sequence to a plastid expression vector pYY12 (Wu et al, 2017). The pYY12 was digested using NcoI and XbaI (Takara, Japan) and then cotransformed with AtGR2 PCR product into E. coli (XL10-Gold, Agilent technologies) competent cells to generate plastid transformation construct pLSC5 (Wu et al, 2017) ( Figure 1A ).…”
Section: Methodsmentioning
confidence: 99%
“…The primers harbor (underlined) a short homologous sequence to a plastid expression vector pYY12 (Wu et al, 2017). The pYY12 was digested using NcoI and XbaI (Takara, Japan) and then cotransformed with AtGR2 PCR product into E. coli (XL10-Gold, Agilent technologies) competent cells to generate plastid transformation construct pLSC5 (Wu et al, 2017) ( Figure 1A ).…”
Section: Methodsmentioning
confidence: 99%
“…Direct engineering of plants, even if implementing the most advanced methods [ 118 , 119 , 120 ], might be impractical because of their lengthy regeneration times and the sheer size of facilities to house large-scale screens. A far more progressive approach would be to test plant-targeted bioengineering designs in heterologous organisms that would be easy to manipulate, capable of rapidly generating large populations, and suitable for massive functional analysis in parallel [ 64 , 121 , 122 , 123 ].…”
Section: A Roadmap For Research On Adapting Life To Marsmentioning
confidence: 99%