In Vivo Assessment of a Hydroxyapatite/κ-Carrageenan–Maleic Anhydride–Casein/Doxorubicin Composite-Coated Titanium Bone Implant

Abstract

Here, we focus on the fabrications of an osteosarcoma implant for bone repair via the development of a hydroxyapatite/κ-carrageenan–maleic anhydride/casein with doxorubicin (HAP/κ-CA-MA-CAS/DOX) composite-deposited titanium (Ti) plate. The HAP/κ-CA-MA-CAS/DOX material was coated on the Ti plate through the EPD method (electrophoretic deposition), applying direct current (DC) signals to deposit the composite on the surface of the Ti plate. The physicochemical and morphological possessions and biocompatibility in vitro of the prepared nanocomposite were examined to assess its prospective effectiveness for purposes of bone regeneration. Excellent biocompatibility and elevated osteoconductivity were confirmed using MG63 osteoblast-like cells. In vivo studies were performed at tibia sites in Wistar rats, and rapid bone regeneration was detected at four weeks in defective bone. Overall, the studies demonstrate that the HAP/κ-CA-MA-CAS/DOX composite enhances the biocompatible and cell-stimulating biointerface of Ti metallic implants. As such, HAP/κ-CA-MA-CAS/DOX implants are viable prospects for osteosarcoma-affected bone regeneration.

Ficus carica extract impregnated amphiphilic polymer scaffold for diabetic wound tissue regenerations

“…Recently, the active wound healing dressing materials containing polymers, naturally occurring plant compounds, and nanoparticles were effectively used. Polymers are majorly applied to treat wound tissue engineering, and it can significantly affect natural derived bioactive polymers, naturally derived inert polymers and biodegradable synthetic polymers [8][9][10].…”

Preparation and Characterization of κ-Carrageenan Modified with Maleic Anhydride and Its Application in Films

“…FT-IR was used to investigate the changes in κCar after modification, as shown in Figure 2 a. The characteristic absorption peaks of κCar appearing at 845 and 930 cm −1 contributed to the C–H bending of substituted d -galactose-4-sulfate and the C–O bond of 3,6-anhydro- d -galactose (3,6-AG), respectively [ 12 ]. The extremely broad band at approximately 3440 cm −1 corresponded to the vibration of the hydroxyl groups (O–H).…”

“…MAH-esterified starch, which was produced by using corn starch as the material and MAH as the esterifying agent via a dry method, could increase the length of the starch side chain and consequently enhance thermoplastic characteristics [ 10 ]. The modification of κCar with MAH has been reported [ 11 , 12 ]. However, reports on the modification of κCar with MAH only and focusing on its physicochemical properties, such as gelling and thermal properties, are almost limited (to the best of the authors’ knowledge).…”

In-vivo Investigations of Hydroxyapatite/Co-polymeric Composites Coated Titanium Plate for Bone Regeneration

“…The first inorganic module of human hard tissue, hydroxyapatite (HAP), has fascinated widespread significance in biomedical and scientific relevance, particularly in bone regenerations (Bose Susmita et al, 2010 ). The HAP reveals a superior suitable act because of the nanometer range's more specific surface area, with the supportive enhancement of various biological goods to interact bone cell and tissues (Zhou et al, 2019 ; Sumathra et al, 2020 ). Besides, HAP coating on Ti plate can reduce the implant fiction time, improve attachment amid implants and host tissues, and generate consistent bone augmentation close to the bone-implant boundary (Ghosh et al, 2019 ).…”