In vivo assessment of silver nanoparticle induced reactive oxygen species reveals tissue specific effects on cellular redox status in the nematode Caenorhabditis elegans

Rossbach, Lisa Magdalena; Oughton, Deborah; Maremonti, Erica; Coutris, Claire; Brede, Dag Anders

doi:10.1016/j.scitotenv.2020.137665

Cited by 15 publications

(7 citation statements)

References 45 publications

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“…For the in vivo assessment of cerium onto the redox homeostasis, the Sod-1, HyPer, and GRX strains, nematodes were sampled at 72 h of exposure. Samples were analyzed as previously described by Rossbach et al 21 and Maremonti et al 22 …”

Section: Methodsmentioning

confidence: 99%

“…C. elegans is, due to its transparency and relatively small size, a suitable model for in vivo redox sensor based on fluorescent probe measurements − and imaging the distribution of ingested metals and metallic nanomaterials in the whole organism or individual organs. ,− Whole body investigation by two-dimensional (2D) X-ray fluorescence (XRF) tomography at synchrotron beamline facilities, with submicron spatial resolutions, may therefore take advantage of these features to identify ingested NPs within tissues, organs, and eggs . Such noninvasive imaging techniques may provide vital information on internal distributions and interactions of metal NPs with tissues and organs and help explain toxic effects …”

Section: Introductionmentioning

confidence: 99%

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Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans

Rossbach

Brede

Nuyts

et al. 2022

Environ. Sci. Technol.

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A combination of synchrotron radiation-based elemental imaging, in vivo redox status analysis, histology, and toxic responses was used to investigate the uptake, biodistribution, and adverse effects of Ce nanoparticles (CeO 2 NP; 10 nm; 0.5–34.96 mg Ce L –1 ) or Ce(NO 3 ) 3 (2.3–26 mg Ce L –1 ) in Caenorhabditis elegans . Elemental mapping of the exposed nematodes revealed Ce uptake in the alimentary canal prior to depuration. Retention of CeO 2 NPs was low compared to that of Ce(NO 3 ) 3 in depurated individuals. X-ray fluorescence (XRF) mapping showed that Ce translocation was confined to the pharyngeal valve and foregut. Ce(NO 3 ) 3 exposure significantly decreased growth, fertility, and reproduction, caused slightly reduced fecundity. XRF mapping and histological analysis revealed severe tissue deformities colocalized with retained Ce surrounding the pharyngeal valve. Both forms of Ce activated the sod-1 antioxidant defense, particularly in the pharynx, whereas no significant effects on the cellular redox balance were identified. The CeO 2 NP-induced deformities did not appear to impair the pharyngeal function or feeding ability as growth effects were restricted to Ce(NO 3 ) 3 exposure. The results demonstrate the utility of integrated submicron-resolution SR-based XRF elemental mapping of tissue-specific distribution and adverse effect analysis to obtain robust toxicological evaluations of metal-containing contaminants.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans

Rossbach

Brede

Nuyts

et al. 2022

Environ. Sci. Technol.

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“…It is known that they can induce oxidative damage, which activates the antioxidant defense systems, including enzymes and low-molecular non-enzymatic antioxidants 63 , 64 . It has been observed in C. elegans that Ag-NPs activate the transcriptional activity of sod-1 (also known as Cu–Zn SOD ) 65 , while upregulation of Sod2 (also known as Mn-SOD ) has been observed in Drosophila melanogaster , also affecting hemocytes 66 . The induction of both SOD genes has also been described in the aquatic larvae of Chironomus riparius in response to Ag-NPs 67 .…”

Section: Discussionmentioning

confidence: 99%

Effect of silver nanoparticles on gene transcription of land snail Helix aspersa

Turki

Younes

Sakly

et al. 2022

Sci Rep

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Silver nanoparticles (Ag-NPs) are extremely useful in a diverse range of consumer goods. However, their impact on the environment is still under research, especially regarding the mechanisms involved in their effect. Aiming to provide some insight, the present work analyzes the transcriptional activity of six genes (Hsp83, Hsp17.2, Hsp19.8, SOD Cu–Zn, Mn-SOD, and BPI) in the terrestrial snail Helix aspersa in the presence of different concentrations of Ag-NPs. The animals were exposed for seven days to Lactuca sativa soaked for one hour in different concentrations of Ag-NPs (20, 50, 100 mg/L). The results revealed that the highest concentration tested of Ag-NPs (100 mg/L) led to a statistically significant induction of the Hsp83 and BPI expression in the digestive gland compared to the control group. However, a trend to upregulation with no statistical significance was observed for all the genes in the digestive gland and the foot, while in the hemolymph, the trend was to downregulation. Ag-NPs affected the stress response and immunity under the tested conditions, although the impact was weak. It is necessary to explore longer exposure times to confirm that the effect can be maintained and impact on health. Our results highlight the usefulness of the terrestrial snail Helix aspersa as a bioindicator organism for silver nanoparticle pollution biomonitoring and, in particular, the use of molecular biomarkers of pollutant effect as candidates to be included in a multi-biomarker strategy.

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“…Glutathione reductase (GR) can reduce oxidized glutathione (GSSG) to GSH, which regenerates the antioxidant capacity of the GSH antioxidant system. The ratio of GSH to GSSG reflects the redox state of the organism, while the levels of malondialdehyde (MDA) and protein carbonyl reflect the degree of lipid peroxidation and protein oxidation, respectively. , …”

Section: Introductionmentioning

confidence: 99%

Synthesis and Photoactivated Toxicity of 2-Thiophenylfuranocoumarin Induce Midgut Damage and Apoptosis in Aedes aegypti Larvae

Wang

Zhang

et al. 2021

J. Agric. Food Chem.

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Furanocoumarins are photoactive compounds derived from secondary plant metabolites. They possess many bioactivities, including antioxidative, anticancer, insecticidal, and bactericidal activities. Here, we designed a new scheme for synthesizing 2-arylfuranocoumarin derivatives by condensation, esterification, bromination, and Wittig reaction. We found that 2-thiophenylfuranocoumarin (Iy) had excellent photosensitive activity. Three Iy concentrations (LC25, LC50, and LC75) were used to treat the fourth instar larvae of Aedes aegypti (A. aegypti). The photoactivated toxicity, sublethal dose, mitochondrial dysfunction, oxidative stress level, intestinal barrier dysfunction, and apoptosis were studied. The results showed that Iy induced reactive oxygen species (ROS) production in midgut cells under ultraviolet light. Ultrastructural analysis demonstrated that mitochondria were damaged, and the activities of related enzymes were inhibited. Ultimately, Iy exposure led to excessive ROS production followed by the inhibition of antioxidant enzymes, including SOD, CAT, GPx, and GR, which diminished ROS elimination and escalated oxidative stress in midgut cells, aggravating the degree of oxidative damage in these cells. Histopathological changes were observed in the midgut, which led to intestinal barrier dysfunction. When the elimination of ROS was blocked and it accumulated in cells, apoptosis-related genes, including AeDronc, AeCaspase7, and AeCaspase8, were induced and activated. In addition, Iy affected the growth and development of A. aegypti at sublethal concentrations, and there was an obvious post-lethal effect. Thus, we found that Iy caused midgut damage and apoptosis in A. aegypti larvae under ultraviolet light, which preliminarily revealed the mode of action of Iy in A. aegypti.

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In vivo assessment of silver nanoparticle induced reactive oxygen species reveals tissue specific effects on cellular redox status in the nematode Caenorhabditis elegans

Cited by 15 publications

References 45 publications

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans

Effect of silver nanoparticles on gene transcription of land snail Helix aspersa

Synthesis and Photoactivated Toxicity of 2-Thiophenylfuranocoumarin Induce Midgut Damage and Apoptosis in Aedes aegypti Larvae

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In vivo assessment of silver nanoparticle induced reactive oxygen species reveals tissue specific effects on cellular redox status in the nematode Caenorhabditis elegans

Cited by 15 publications

References 45 publications

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO2 NP-Exposed Caenorhabditis elegans

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO2 NP-Exposed Caenorhabditis elegans

Effect of silver nanoparticles on gene transcription of land snail Helix aspersa

Synthesis and Photoactivated Toxicity of 2-Thiophenylfuranocoumarin Induce Midgut Damage and Apoptosis in Aedes aegypti Larvae

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Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans

Synchrotron XRF Analysis Identifies Cerium Accumulation Colocalized with Pharyngeal Deformities in CeO₂ NP-Exposed Caenorhabditis elegans