In vivo biocompatibility and mechanical properties of porous zein scaffolds

Wang, Huajie; Gong, Shengju; Lin, Zhixin; Fu, Jian-Xi; Xue, Shengjun; Huang, Jin; Wang, Jin‐Ye

doi:10.1016/j.biomaterials.2007.05.017

Cited by 109 publications

(102 citation statements)

References 39 publications

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“…Zein, a water insoluble hydrophobic storage protein found in corn and maize, attracts a particular interest as a biopolymer since it has good film forming properties, biodegradability and biocompatibility, and it is the major co-product of the oil industry and rapidly growing bioethanol industry (Selling, Woods, Sessa, & Biswas, 2008;Shukla & Cheryan, 2001;Wang et al, 2007;Zhang, Luo, & Wang, 2011). In foods, zein is applied mainly as a coating material for candies, fresh and dried fruits and nuts and used as an ingredient in chewing gum production (Bai, Alleyne, Hagenmaier, Mattheis, & Baldwin, 2003;Lai & Padua, 1997;Shukla & Cheryan, 2001).…”

Section: Introductionmentioning

confidence: 99%

Incorporating phenolic compounds opens a new perspective to use zein films as flexible bioactive packaging materials

Arcan

Yemenicioğlu

2011

Food Research International

194

108

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To eliminate their classical brittleness and flexibility problems zein films were plasticized by incorporation of different phenolic acids (gallic acid (GA), p-hydroxy benzoic acid (HBA) or ferulic acids (FA)) or flavonoids (catechin (CAT), flavone (FLA) or quercetin (QU)). The use of GA, CAT, FA and HBA at 3 mg/cm 2 eliminated the brittleness of films and gave highly flexible films showing elongations between 135% and 189%, while FLA and QU caused no considerable effect on film elongation. The films containing FA and HBA showed extreme swelling and lost their structural integrity when hydrated in distilled water. In contrast, CAT and GA containing films maintained their integrity following hydration. Most of the GA (up to 93%) and a considerable portion of CAT (up to 60%) in the films existed in soluble form. Therefore, the films showed antioxidant and/or antimicrobial activity. The TEACs of soluble phenolic compounds in 3 mg/cm 2 CAT and GA containing films were 21.0 and 86.2 μmol trolox/cm 2 , respectively. The GA containing films showed antimicrobial activity on Listeria monocytogenes and Campylobacter jejuni, while CAT showed no antimicrobial activity on these bacteria at the studied concentration. This work opens a new perspective for using zein in flexible bioactive packaging.

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Section: Introductionmentioning

confidence: 99%

Incorporating phenolic compounds opens a new perspective to use zein films as flexible bioactive packaging materials

Arcan

Yemenicioğlu

2011

Food Research International

194

108

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“…Zein, a water insoluble hydrophobic storage protein found in corn and maize, attracts a particular interest as a biopolymer since it has excellent film forming and gas barrier properties; it is one of the rare proteins soluble in various organic solvents including ethanol and it is the major co-product of the oil and rapidly growing bioethanol industries (Manley & Evans, 1943;Selling, Woods, Sessa, & Biswas, 2008;Shukla & Cheryan, 2001;Wang et al, 2007;Zhang, Luo, & Wang, 2011). Thus, a particular interest has been focused on use of zein in active food packaging by incorporation of different natural antimicrobials including lysozyme (Gucbilmez et al, 2007;Mecitoglu et al, 2006;Padgett et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Development of flexible zein–wax composite and zein–fatty acid blend films for controlled release of lysozyme

Arcan

Yemenicioğlu

2013

Food Research International

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The aim of this study was controlled release of lysozyme by modification of hydrophobicity and morphology of zein films using composite and blend film making methods. The incorporation of beeswax, carnauba or candelilla wax into films at 5% (w/w) of zein gave composite films containing amorphous wax particles, while incorporation of oleic acid into film at 5% of zein caused formation of blend films containing many spherical zein capsules within their matrix. The lysozyme release rates of composites reduced as the melting point of waxes increased. The composites and blends showed 2.5 to 17 fold lower lysozyme release rates than controls. The films were effectively plasticized by using catechin. The catechin also provided antioxidant activity of films (up to 69 μmol Trolox/cm 2 ) and contributed to their controlled release properties by reducing film porosity. The films showed antimicrobial activity against Listeria innocua. This work showed the possibility of obtaining advanced edible films having flexibility, antimicrobial and antioxidant activity and controlled release properties.

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“…Heparin-loaded zein microsphere film and its degraded product had better biocompatibility when they were used to culture human umbilical vein endothelial cells . Gong (Gong et al, 2006) further demonstrated that zein scaffold can promote rat bone marrow stromal cell adhesion, growth, and differentiation, suggesting that zein protein possesses good histocompatibility (Wang et al, 2007). Here zein as a scaffold material was studied for periodontal tissue engineering.…”

Section: Discussionmentioning

confidence: 97%

The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering

Chen

et al. 2010

Int J Oral Sci

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Aim Tissue engineering is a promising area with a broad range of applications in the fields of regenerative medicine and human health. The emergence of periodontal tissue engineering for clinical treatment of periodontal disease has opened a new therapeutic avenue. The choice of scaffold is crucial. This study was conducted to prepare zein scaffold and explore the suitability of zein and Shuanghuangbu for periodontal tissue engineering. Methodology A zein scaffold was made using the solvent casting/particulate leaching method with sodium chloride (NaCl) particles as the porogen. The physical properties of the zein scaffold were evaluated by observing its shape and determining its pore structure and porosity. Cytotoxicity PDLCs. Altogether, these findings provide the basis for in vivo testing on animals.

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In vivo biocompatibility and mechanical properties of porous zein scaffolds

Cited by 109 publications

References 39 publications

Incorporating phenolic compounds opens a new perspective to use zein films as flexible bioactive packaging materials

Incorporating phenolic compounds opens a new perspective to use zein films as flexible bioactive packaging materials

Development of flexible zein–wax composite and zein–fatty acid blend films for controlled release of lysozyme

The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering

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