In Vivo Evaluation of a Novel Implant Coating Agent: Laminin‐1

Bougas, Kostas; Jimbo, Ryo; Vandeweghe, Stefan; Tovar, Nick; Baldassarri, Marta; Alenezi, Ali; Coelho, Paulo G.; Wennerberg, Ann

doi:10.1111/cid.12037

Cited by 14 publications

(7 citation statements)

References 34 publications

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“…In this context, in vitro studies have indicated that laminin-1 can selectively recruit osteoprogenitors through an integrin β1-dependent cell attachment effect [18]. Moreover, previous in vivo examinations reported that laminin stimulates osteointegration [19]. The areas of wall restructuring did not show any calcification, whereas parts far from the anastomoses were more susceptible to calcification, which may be due to enhanced calcium binding to the extracellular matrix, particularly to damaged elastin fibers [20], in the absence of matrix-producing cells.…”

Section: Discussionmentioning

confidence: 99%

Influence of Laminin Coating on the Autologous In Vivo Recellularization of Decellularized Vascular Protheses

et al. 2019

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Decellularization of non-autologous biological implants reduces the immune response against foreign tissue. Striving for in vivo repopulation of aortic prostheses with autologous cells, thereby improving the graft biocompatibility, we examined surface coating with laminin in a standardized rat implantation model. Detergent-decellularized aortic grafts from donor rats (n = 37) were coated with laminin and systemically implanted into Wistar rats. Uncoated implants served as controls. Implant re-colonization and remodeling were examined by scanning electron microscopy (n = 10), histology and immunohistology (n = 18). Laminin coating persisted over eight weeks. Two weeks after implantation, no relevant neoendothelium formation was observed, whereas it was covering the whole grafts after eight weeks, with a significant acceleration in the laminin group (p = 0.0048). Remarkably, the intima-to-media ratio, indicating adverse hyperplasia, was significantly diminished in the laminin group (p = 0.0149). No intergroup difference was detected in terms of medial recellularization (p = 0.2577). Alpha-smooth muscle actin-positive cells originating from the adventitial surface invaded the media in both groups to a similar extent. The amount of calcifying hydroxyapatite deposition in the intima and the media did not differ between the groups. Inflammatory cell markers (CD3 and CD68) proved negative in coated as well as uncoated decellularized implants. The coating of decellularized aortic implants with bioactive laminin caused an acceleration of the autologous recellularization and a reduction of the intima hyperplasia. Thereby, laminin coating seems to be a promising strategy to enhance the biocompatibility of tissue-engineered vascular implants.

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Section: Discussionmentioning

confidence: 99%

Influence of Laminin Coating on the Autologous In Vivo Recellularization of Decellularized Vascular Protheses

et al. 2019

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“…However, these studies primarily used smooth Ti surface implants for in vitro and in vivo experiments. Only two experiments assessed the effect of a laminin coating on modified Ti surfaces, and these modifications comprised a hydroxyapatite coating and alkali and heat treatments (Bougas et al, , ). In contrast, we assessed the effect of treating SLA surfaces with a human laminin α2 chain‐derived peptide on the osseointegration of implants.…”

Section: Discussionmentioning

confidence: 99%

A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

Choi

Kim

et al. 2020

J Biomedical Materials Res

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Early implant loading is very important for reducing the duration of missing teeth in human patients. The laminin‐derived peptide, DLTIDDSYWYRI motif (Ln2‐P3), accelerates bone healing. Therefore, to investigate the hypothesis that Ln2‐P3 increases the bone response to sandblasted, large‐grit, acid‐etched (SLA) titanium implants, the effect of the Ln2‐P3 peptide on the osseointegration of SLA titanium implants was evaluated in vitro and in vivo. Human osteoblast‐like cells were cultured on untreated, scrambled peptide (SP)‐treated, and Ln2‐P3‐treated SLA titanium discs, and the cellular responses of these cells were evaluated. The Ln2‐P3 treatment augmented osteoblast attachment and spreading, alkaline phosphatase activity, and the expression of osteogenic marker genes. Furthermore, the untreated and Ln2‐P3‐treated SLA titanium implants were inserted into the tibiae of rabbits for 9 and 11 days. Compared with the untreated implants, the Ln2‐P3‐treated implants showed a significantly higher bone‐to‐implant contact ratio at Day 9 after implantation and an increased bone area. The Ln2‐P3 treatment of the SLA titanium implant surface augmented osteoblastic activity and accelerated peri‐implant bone formation at the bone–implant interface. Overall, these results indicated that compared with the SLA titanium surface alone, the Ln2‐P3 peptide‐treated SLA titanium surface enhances initial osseointegration, thereby facilitating earlier implant loading.

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“…More importantly, our data suggest that LN-1 could be a promising biocompatible material for use in dental tissue regeneration. Indeed, in vivo tests of LN-1 have been conducted to evaluate its potential use for integration of implants with alveolar bone: Bougas et al demonstrated that LN-1-coated implants had a significant higher removal torque, and that the area of bone surrounding the implant was dramatically higher after 4 weeks, suggesting that LN-1 would have considerable potential for use as a coating agent for implant surface modification (34). However, due to differences in animal models and evaluation systems, in vivo results obtained using LN-1-coated implants vary from one group to another, and the in vivo conditions do not completely recapitulate the actual scenario in the human body.…”

Section: Discussionmentioning

confidence: 99%

Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype

Tang

Saito

2018

J Oral Sci

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The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner. Moreover, it enhanced ALP activity and expression of key odontogenic genes (DMP-1 and DSPP) upon addition of mineralization reagents, leading to significant promotion of calcification by the cells. These results demonstrate that LN-1 acts as an adhesive for odontoblast-like cells, allowing up-regulation of odontogenic genes and accelerating matrix mineralization. In the context of the present study, the optimal LN-1 coating concentration for MDPC-23 cells was suggested to be 100 µg/mL.

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In Vivo Evaluation of a Novel Implant Coating Agent: Laminin‐1

Abstract: Within the limitations of this study, our results suggest a great potential for laminin-1 as a coating agent. A turned implant surface coated with laminin-1 could enhance osseointegration comparable with a bioactive implant surface while keeping the surface smooth.

Cited by 14 publications

References 34 publications

Influence of Laminin Coating on the Autologous In Vivo Recellularization of Decellularized Vascular Protheses

Influence of Laminin Coating on the Autologous In Vivo Recellularization of Decellularized Vascular Protheses

A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype

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