2000
DOI: 10.1016/s0168-9452(00)00195-3
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In vivo evaluation of the context sequence of the translation initiation codon in plants

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Cited by 86 publications
(70 citation statements)
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References 41 publications
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“…In all cases, the 5 0 -proximal ORF, ORF1, encodes a protein of *27 kDa (p27) that shows homology to auxiliary replication proteins of tombusvirids [5,16,24,25], such as those of carmoviruses (Table 2). Each ORF1 begins with AUG in a favorable translation context (A or G at positions -3 and ?4, with the exception of PCRPV ORF1, which has a U at position ?4) [18,21] and terminates at a UAG codon. This amber codon is in all cases surrounded by an almost identical sequence (aaaUAGggg/a) that is consistent with consensus motifs for type III readthrough elements [10].…”
Section: Genomic Organization and Coding Propertiesmentioning
confidence: 99%
“…In all cases, the 5 0 -proximal ORF, ORF1, encodes a protein of *27 kDa (p27) that shows homology to auxiliary replication proteins of tombusvirids [5,16,24,25], such as those of carmoviruses (Table 2). Each ORF1 begins with AUG in a favorable translation context (A or G at positions -3 and ?4, with the exception of PCRPV ORF1, which has a U at position ?4) [18,21] and terminates at a UAG codon. This amber codon is in all cases surrounded by an almost identical sequence (aaaUAGggg/a) that is consistent with consensus motifs for type III readthrough elements [10].…”
Section: Genomic Organization and Coding Propertiesmentioning
confidence: 99%
“…GCCAUGGC and RAAAUGGC were shown to be the most-efficient TISs in maize and tobacco cells, respectively. (6) Unlike in vertebrates and plants, in yeast cells, the AUG recognition efficiency varies maximally twofold depending on the nucleotide at position À3 (A>G>C>U; consensus: AAAAAAAAAAUGUC). (7) It was proposed that some other mRNA features can influence the AUG recognition.…”
Section: Eukaryotic Mrnas and Translation Initiation Mechanismsmentioning
confidence: 99%
“…Figure 2B). The translation initiation codon could be identified unambiguously, because it possessed a good context sequence, AAAATGG (Joshi et al., 1997;Lukaszewicz et al, 2000), and was preceded by an in-frame stop codon.…”
Section: Isolation and Characterization Of Npabc1mentioning
confidence: 99%
“…The full-length cDNA contained a 4311-bp open reading frame predicted to encode a protein of 1436 amino acid residues with a size of 161,848 D, in good agreement with the results of SDS-PAGE ( Figure 2B). The translation initiation codon could be identified unambiguously, because it possessed a good context sequence, AAAATGG (Joshi et al, 1997;Lukaszewicz et al, 2000), and was preceded by an in-frame stop codon.…”
Section: Isolation and Characterization Of Npabc1mentioning
confidence: 99%
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