In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis

Jacobs, Danny O.; Maris, John M.; Fried, Robert; Settle, R. Gregg; Rolandelli, Rolando R.; Koruda, Mark J.; Chance, B; Rombeau, John L.

doi:10.1001/archsurg.1988.01400350139022

Cited by 37 publications

(21 citation statements)

References 11 publications

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“…The appearance of phosphomonoesters is unexplained as yet eLP model in rats, P-NMR of skeletal muscle was performed. In survivors, a 40070 decrease in the Per/Pi ratio was observed by 24 h, increasing in severity with time (Jacobs et al 1988). We obtained similar results with P-NMR in the hind leg of mice 12 days after zymosan inoculation (Fig.…”

Section: Experimental Datasupporting

confidence: 82%

Shock, Sepsis, and Multiple Organ Failure: The Result of Whole-Body Inflammation

Goris

1993

Pathophysiology of Shock, Sepsis, and Organ Failure

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Section: Experimental Datasupporting

confidence: 82%

Shock, Sepsis, and Multiple Organ Failure: The Result of Whole-Body Inflammation

Goris

1993

Pathophysiology of Shock, Sepsis, and Organ Failure

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“…Sepsis increases metabolic rate, as reflected in oxygen consumption and overall substrate utilization [201]. However, this is paradoxically associated with a reduction in ATP utilization in many tissues, which occurs in concert with maintenance of ATP abundance, suggesting that the decreased use reflects an attempt to conserve ATP availability [202,203]. Decreased activity in electron transport chain complexes I, III, IV and ATP synthase has also been demonstrated [183,184].…”

Section: Basic/translational Science What Mechanisms Underlie Sepsis-mentioning

confidence: 99%

Surviving sepsis campaign: research priorities for sepsis and septic shock

et al. 2018

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Objective: To identify research priorities in the management, epidemiology, outcome and underlying causes of sepsis and septic shock.Design: A consensus committee of 16 international experts representing the European Society of Intensive Care Medicine and Society of Critical Care Medicine was convened at the annual meetings of both societies. Subgroups had teleconference and electronic-based discussion. The entire committee iteratively developed the entire document and recommendations.Methods: Each committee member independently gave their top five priorities for sepsis research. A total of 88 suggestions (ESM 1 -supplemental table 1) were grouped into categories by the committee co-chairs, leading to the formation of seven subgroups: infection, fluids and vasoactive agents, adjunctive therapy, administration/epidemiology, scoring/identification, post-intensive care unit, and basic/translational science. Each subgroup had teleconferences to go over each priority followed by formal voting within each subgroup. The entire committee also voted on top priorities across all subgroups except for basic/translational science. Results:The Surviving Sepsis Research Committee provides 26 priorities for sepsis and septic shock. Of these, the top six clinical priorities were identified and include the following questions: (1) can targeted/personalized/precision medicine approaches determine which therapies will work for which patients at which times?; (2) what are ideal endpoints for volume resuscitation and how should volume resuscitation be titrated?; (3) should rapid diagnostic tests be implemented in clinical practice?; (4) should empiric antibiotic combination therapy be used in sepsis or septic shock?; (5) what are the predictors of sepsis long-term morbidity and mortality?; and (6) what information identifies organ dysfunction?Conclusions: While the Surviving Sepsis Campaign guidelines give multiple recommendations on the treatment of sepsis, significant knowledge gaps remain, both in bedside issues directly applicable to clinicians, as well as understanding the fundamental mechanisms underlying the development and progression of sepsis. The priorities identified represent a roadmap for research in sepsis and septic shock.

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“…Even in hypocaloric animals, the muscle pH fell by only 0.03. In preliminary studies in starved rats, Jacobs et al (22) concluded that intracellular ATP was maintained at the expense of CrP.…”

Section: Musclefree Mg2+mentioning

confidence: 99%

Effect of dietary manipulations (fasting, hypocaloric feeding, and subsequent refeeding) on rat muscle energetics as assessed by nuclear magnetic resonance spectroscopy.

Pichard¹,

Vaughan²,

Struk³

et al. 1988

J. Clin. Invest.

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31p nuclear magnetic resonance (NMR) spectroscopy in vivo and fluorometry were used to measure muscle ATP, total creatine, pH, and Mg2+ in vivo; and to calculate creatine phosphate (CrP), the ratios-of CrP/inorganic phosphate (Pi), CrP/ ATP, free ADP levels, and the free-energy change in ATP hydrolysis so nutritional effects could be ascertained. These parameters were determined in vivo in resting control, 2-dfasted, and hypocalorically fed rats and in animals similarly hypocalorically fed and then refed. The ATP, Pi, and intracellular Mg2+ levels were comparable in the four groups. When the fasted and underfed animals were compared with the control and refed animals, there were falls in the ratios of CrP/Pj and CrP/ATP, in the calculated CrP, and the free-energy change of ATP hydrolysis, but a rise in the calculated free ADP. In the hypocaloric group, intracellular pH fell significantly and a large peak was noted in the phosphodiester region. The data are consistent with the hypothesis that ATP levels are maintained at the cost of CrP, suggesting that ATP production is disturbed by aerobic and anaerobic mechanisms.

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In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis

Cited by 37 publications

References 11 publications

Shock, Sepsis, and Multiple Organ Failure: The Result of Whole-Body Inflammation

Shock, Sepsis, and Multiple Organ Failure: The Result of Whole-Body Inflammation

Surviving sepsis campaign: research priorities for sepsis and septic shock

Effect of dietary manipulations (fasting, hypocaloric feeding, and subsequent refeeding) on rat muscle energetics as assessed by nuclear magnetic resonance spectroscopy.

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