In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents

Zharov, Vladimir P.; Galanzha, Ekaterina I.; Shashkov, E. V.; Khlebtsov, Nikolai G.; Tuchin, Valery V.

doi:10.1364/ol.31.003623

Cited by 213 publications

(201 citation statements)

References 4 publications

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“…ear of nude mouse ∼270 µm thick), or (2) by decreasing light scattering using a recently developed optical clearing method combined with spectral selection (e.g. use a "green" filter to increase blood vessel contrast) [74][75][76][77] . Figure 1 illustrates our few attempts using these models and transmission microscopy to obtain high-resolution images of individual cells in blood flow without staining.…”

Section: Animal Modelsmentioning

confidence: 99%

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Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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Section: Animal Modelsmentioning

confidence: 99%

“…normal and apoptotic WBCs or cancer cells) in blood and lymph flow in vivo on the basis of their differences in integral and local absorption associated with specific heme proteins [58,77,94,[114][115][116][117][118] .…”

Section: Pt Modulementioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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“…Another method is to use the photothermal/ photoacoustic detection, which also improves the detection depth. Tuchin et al developed a photothermal image flow cytometer to detect target cells in blood and lymph flow in vivo (29)(30)(31). They used nanoparticles to enrich the rare cells and achieved significantly higher sensitivity (32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

Circulation times of prostate cancer and hepatocellular carcinoma cells by in vivo flow cytometry

Guo

Wang

et al. 2011

Cytometry Pt A

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In metastasis, the cancer cells that travel through the body are capable of establishing new tumors in locations remote from the site of the original disease. To metastasize, a cancer cell must break away from its tumor and invade either the circulatory or lymphatic system, which will carry it to a new location, and establish itself in the new site. Once in the blood stream, the cancer cells now have access to every portion of the body. Here, we have used the ''in vivo flow cytometer'' to study if there is any relationship between metastatic potential and depletion kinetics of circulating tumor cells. The in vivo flow cytometer has the capability to detect and quantify continuously the number and flow characteristics of fluorescently labelled cells in vivo. We have improved the counting algorithm and measured the depletion kinetics of cancer cells with different metastatic potential. Interestingly, more invasive PC-3 prostate cancer cells are depleted faster from the circulation than LNCaP cells. In addition, we have measured the depletion kinetics of two related human hepatocellular carcinoma (liver cancer) cell lines, high-metastatic HCCLM3 cells, and low-metastatic HepG2 cells. More than 60% HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, \40% HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes. ' 2011 International Society for Advancement of Cytometry Key terms in vivo flow cytometer; cancer metastasis; circulating tumor cells; prostate cancer; hepatocellular carcinoma; in vivo confocal imaging METASTASIS is a complicated process that has yet to be completely understood. In metastasis, the cancer cells that travel through the body are capable of establishing new tumors in locations remote from the site of the original disease. To metastasize, a cancer cell must break away from its tumor and invade either the circulatory or lymph system (1-3). Once in the blood stream, the cancer cells now have access to every portion of the body. The cancer cells in the bloodstream must fight the body's defense system and try to reattach itself in a new location. Fewer than 1 in 10,000 cancer cells survive circulation to create a new tumor. The circulation of the blood plays an important role in determining where cancer cells travel. The cancer cells usually are trapped in the first set of capillaries that they encounter downstream from their point of entry. These capillaries are often in the lung, since returning deoxygenated venous blood leaving many organs is returned to the lung for reoxygenation. From the intestines, the blood go to the liver first, thus cancer cells leaving the intestines will go there. Therefore, the lung and the liver are the two most common sites for metastasis in the human body. Many circulating cancer cells cannot finish the entire process of metastasis (4).

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“…Maximum CMM thickness (0.5 mm) was measured with a lateral resolution of 45 μm and an axial resolution of 15 μm (Figure 8). Detection of melanoma cells in circulation was also reported (Holan & Viator, 2008;Weight et al, 2006;Zharov et al, 2006). More recently, in a pilot study Song et al proposed that non-invasive in vivo spectroscopic PAI can map sentinel lymph node using gold nanorods as lymph node tracers in a rodent model (Song et al, 2009).…”

Section: Photoacoustic Imaging (Pai)mentioning

confidence: 99%

Determination of Melanoma Lateral and Depth Margins: Potential for Treatment Planning and Five-Year Survival Rate

Wang¹,

Qiu²,

Milner³

2011

Skin Cancer Overview

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In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents

Cited by 213 publications

References 4 publications

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Circulation times of prostate cancer and hepatocellular carcinoma cells by in vivo flow cytometry

Determination of Melanoma Lateral and Depth Margins: Potential for Treatment Planning and Five-Year Survival Rate

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