In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach

Jeon, Seungwan; Song, Hyun Beom; Kim, Jae-Woo; Lee, Byung‐Joo; Managuli, Ravi; Kim, Jin Hyoung; Kim, Jeong Hun; Kim, Chulhong

doi:10.1038/s41598-017-04334-z

Cited by 88 publications

(54 citation statements)

References 35 publications

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“…Tight optical focusing will further improve the lateral resolution for PAM and resolution up to 0.32 µm has been reported using OR-PAM [13]. The imaging depth in OR-PAM is limited by optical transport mean free path (~1 mm inside the biological tissue) [14][15][16]. In OR-PAM, the optical delivery is mainly through a SM fiber.…”

Section: Introductionmentioning

confidence: 99%

Optical resolution photoacoustic microscopy based on multimode fibers

Moothanchery

Kim

et al. 2018

Biomed. Opt. Express

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Photoacoustic microscopy (PAM) is a multiscale imaging technique. In optical-resolution photoacoustic microscopy (OR-PAM), a single mode (SM) fiber is normally used as the source of optical excitation to be focused into a diffraction-limited spot. Recent advances in OR-PAM have improved its imaging speed using microelectromechanical systems (MEMS). Here we report for the first time the use of a multimode (MM) fiber as the optical excitation source for high resolution OR-PAM imaging. A high-speed MEMS scanner based OR-PAM system combined with the mechanical movement to provide wide area imaging was used. The use of multimode fiber for achieving tight optical focus would make the optical alignment easier and high repetition rate light delivery possible for high-speed OR-PAM imaging. A lateral resolution of 3.5 µm and axial resolution of 27 µm with ~1.5 mm imaging depth was successfully demonstrated using the system. The efficacy of multimode fibers for achieving tight focus is beneficial for developing high-resolution photoacoustic endoscopy systems and can be combined with other optical endoscopic imaging modalities as well.

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Section: Introductionmentioning

confidence: 99%

Optical resolution photoacoustic microscopy based on multimode fibers

Moothanchery

Kim

et al. 2018

Biomed. Opt. Express

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“…However, the imaging depth is limited (700 μm) and the depth of variation of corneal vessels can be more than 1.4 mm owing to the curvature of the mouse cornea. Jeon et al [17] have demonstrated a new method to evaluate blood circulation in the eye by combining in vivo PAM imaging. By using a machine learning algorithm, random sample consensus algorithm (RANSAC), they could have visualize the PA anterior ocular vasculature image and demonstrate layer-by-layer analysis of corneal neovascularization on a mouse eye (Figure 3c-e).…”

Section: Corneal Neovascularizationmentioning

confidence: 99%

Photoacoustic Imaging of the Eye

Li¹,

Paulus²

2020

Photoacoustic Imaging - Principles, Advances and Applications

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Photoacoustic imaging (PAI) is a novel, hybrid, non-ionizing, and non-invasive imaging technology with high-resolution, high sensitivity, high-contrast, and high depth of penetration. Hence, it has particularly useful applications in eye investigations. It can provide both anatomic and functional ocular characterizations. Many eye diseases, including macular degeneration and diabetic retinopathy, involve abnormalities in the vasculature, and thus the ability of PAI to affectively visualize the vasculature can be incredibly helpful to evaluate normal and disease states of the eye. In future research, PAI of the eye can be dramatically improved in terms of its resolution, use of contrast agents for molecular imaging, safety evaluations to develop a clinically approved system, and integration with existing fundus imaging modalities. Multimodality ocular imaging platforms have also been successfully developed by a combination of photoacoustic microscopy (PAM) with other optical imaging such as optical coherence tomography (OCT), scanning laser ophthalmoscopy (SLO), and fluorescence microscopy (FM). The multimodal images can accurately be acquired from a single imaging system and co-registered on the same image plane, enabling improved evaluation of eye disease states. In this book chapter, the potential application of photoacoustic imaging of the eye in both research and clinical diagnosis are comprehensively discussed as a powerful medical screening technique for visualization of various ocular diseases.

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“…Based on system designs to yield different resolutions and penetration depth, PAM can be classified into optical-resolution (OR) and acoustic-resolution (AR) PAM. OR-PAM uses tight optical focusing 8,9 to achieve a high lateral resolution of 0.32 μm and penetration depth of up to 1 mm. [10][11][12] AR-PAM employs tight acoustic focusing and weak optical focusing to achieve imaging depth of up to 3 mm (>optical mean free path of 1 mm) with a lateral resolution of 45 μm at 570 nm.…”

Section: Introductionmentioning

confidence: 99%

High-speed simultaneous multiscale photoacoustic microscopy

Moothanchery

Kim

et al. 2019

J. Biomed. Opt.

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Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides highresolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of 5 μm and penetration depth ∼0.9-mm in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of 84 μm and penetration depth of ∼2-mm in vivo was achieved using AR-PAM at 532 nm. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach

Cited by 88 publications

References 35 publications

Optical resolution photoacoustic microscopy based on multimode fibers

Optical resolution photoacoustic microscopy based on multimode fibers

Photoacoustic Imaging of the Eye

High-speed simultaneous multiscale photoacoustic microscopy

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