In Vivo Surface‐Enhanced Transmission Raman Spectroscopy under Maximum Permissible Exposure: Toward Photosafe Detection of Deep‐Seated Tumors

Chen, Ruoyu; Liu, Fugang; Miao, Peng; Liu, Lin; Ye, Jian

doi:10.1002/smtd.202201334

Cited by 29 publications

(39 citation statements)

References 48 publications

(92 reference statements)

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“…Our work began with theoretical calculations to investigate how the Raman peak ratio changed with Raman photon propagation distance in tissue (Figure 2A). As in our previous work, 13 we apply the steady‐state radiative transport equations (RTE) to model the field inside a homogeneous medium for incident light (see Section 4 for more details). Since continuous wave (CW) illumination and transmissive detection were applied in TRS, the excitation on the surface of SERS nanotags and Raman photon propagation through the tissue can also be modeled using a diffusion approximation within an RTE framework.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure S3a, the intensity of the Raman peak at 520 cm −1 shows significantly different attenuations with depth compared to that at 1580 cm −1 , although the whole Raman signal shows a U shape, which has been previously reported by us. 13 The spectra were then normalized to the intensity of the Raman peak at 1580 cm −1 to better visualize their attenuation trends. As shown in Figure 2G, we find that the normalized intensity of the Raman peak at 520 cm −1 increases continuously with increasing depth.…”

Section: Linear Relationship Between Rpr and Depth Of Sers Phantom In...mentioning

confidence: 99%

“…10a,11b,12 Very recently, we have achieved deep TRS detection of SERS nanotags through up to 14-cm ex vivo biological tissue, which is close to the thickness of an adult human body. 13 Several TRS-based approaches have been recently explored to estimate the depth of phantom lesions in turbid media. For example, depth information was obtained with prior knowledge of computed tomography images.…”

Section: Introductionmentioning

confidence: 99%

“…TRS detection has been explored for the diagnosis of lesions such as breast cancers 10a,11b,12 . Very recently, we have achieved deep TRS detection of SERS nanotags through up to 14‐cm ex vivo biological tissue, which is close to the thickness of an adult human body 13 . Several TRS‐based approaches have been recently explored to estimate the depth of phantom lesions in turbid media.…”

Section: Introductionmentioning

confidence: 99%

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A rapid and universal method for depth estimation of lesions in heterogeneous tissues via photosafe ratiometric transmission Raman spectroscopy

Liu

2023

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Noninvasive detection of deep lesions remains a long‐standing goal for clinical applications, with depth estimation of a single lesion in heterogeneous tissues being a key challenge. Currently, optical techniques are widely applied for lesion detection, but they face difficulties in achieving rapid and precise lesion depth estimation, particularly when the lesions are buried in thick heterogeneous tissues and the applied irradiance is below clinically maximum permissible exposure. Herein, we theoretically and experimentally demonstrate a universal method for depth prediction of phantom lesions labeled with surface‐enhanced Raman scattering nanotags in thick biological tissues using ratiometric transmission Raman spectroscopy (TRS). We begin by highlighting the linear relationship between the natural logarithm of Raman peak‐to‐peak ratio and the lesion depth and establishing a home‐built TRS system with the clinically safe irradiance. We achieve an accurate depth prediction for phantom lesions hidden in 6‐cm‐thick ex vivo homogeneous tissue with a root mean squared error (RMSE) as low as 2.42%. Additionally, we predict the depth of phantom lesions buried in 5‐cm‐thick ex vivo heterogeneous tissues with an RMSE of down to 8.35%. We also demonstrate the applicability of this method theoretically for highly heterogeneous tissues such as complex in vivo environments. This work provides a rapid, robust, and universal method to estimate the depth of lesions in complex biological samples, demonstrating the potential of ratiometric Raman spectroscopy for lesion localization in clinical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Linear Relationship Between Rpr and Depth Of Sers Phantom In...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A rapid and universal method for depth estimation of lesions in heterogeneous tissues via photosafe ratiometric transmission Raman spectroscopy

Liu

2023

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“…It is embedded with IR-780 NIR resonance reporter and can be used for long-term and high-speed live cell tracking imaging due to reduced photodamage to cells. The combination of this SERS probe with transmission Raman spectroscopy (TRS) also enables the non-invasive and light-safe detection of "phantom" lesions hidden deep in biological tissues [ 208 ]. Due to the strong optical scattering and absorption in biological tissues, Raman signal is usually limited by the shallow depth of tissue penetration, which largely limits its application in in-vivo biomedical detection of deep lesions.…”

Section: Biosensing Applications In Several Important Bio-fieldsmentioning

confidence: 99%

Recent development of surface-enhanced Raman scattering for biosensing

Lin

Peng

et al. 2023

J Nanobiotechnol

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Surface-Enhanced Raman Scattering (SERS) technology, as a powerful tool to identify molecular species by collecting molecular spectral signals at the single-molecule level, has achieved substantial progresses in the fields of environmental science, medical diagnosis, food safety, and biological analysis. As deepening research is delved into SERS sensing, more and more high-performance or multifunctional SERS substrate materials emerge, which are expected to push Raman sensing into more application fields. Especially in the field of biological analysis, intrinsic and extrinsic SERS sensing schemes have been widely used and explored due to their fast, sensitive and reliable advantages. Herein, recent developments of SERS substrates and their applications in biomolecular detection (SARS-CoV-2 virus, tumor etc.), biological imaging and pesticide detection are summarized. The SERS concepts (including its basic theory and sensing mechanism) and the important strategies (extending from nanomaterials with tunable shapes and nanostructures to surface bio-functionalization by modifying affinity groups or specific biomolecules) for improving SERS biosensing performance are comprehensively discussed. For data analysis and identification, the applications of machine learning methods and software acquisition sources in SERS biosensing and diagnosing are discussed in detail. In conclusion, the challenges and perspectives of SERS biosensing in the future are presented.

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Non‐Invasive Detection, Precise Localization, and Perioperative Navigation of In Vivo Deep Lesions Using Transmission Raman Spectroscopy

Deng

Zhou

et al. 2023

Advanced Science

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Non‐invasive detection and precise localization of deep lesions have attracted significant attention for both fundamental and clinical studies. Optical modality techniques are promising with high sensitivity and molecular specificity, but are limited by shallow tissue penetration and the failure to accurately determine lesion depth. Here the authors report in vivo ratiometric surface‐enhanced transmission Raman spectroscopy (SETRS) for non‐invasive localization and perioperative surgery navigation of deep sentinel lymph nodes in live rats. The SETRS system uses ultrabright surface‐enhanced Raman spectroscopy (SERS) nanoparticles with a low detection limit of 10 pM and a home‐built photosafe transmission Raman spectroscopy setup. The ratiometric SETRS strategy is proposed based on the ratio of multiple Raman spectral peaks for obtaining lesion depth. Via this strategy, the depth of the phantom lesions in ex vivo rat tissues is precisely determined with a mean‐absolute‐percentage‐error of 11.8%, and the accurate localization of a 6‐mm‐deep rat popliteal lymph node is achieved. The feasibility of ratiometric SETRS allows the successful perioperative navigation of in vivo lymph node biopsy surgery in live rats under clinically safe laser irradiance. This study represents a significant step toward the clinical translation of TRS techniques, providing new insights for the design and implementation of in vivo SERS applications.

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In Vivo Surface‐Enhanced Transmission Raman Spectroscopy under Maximum Permissible Exposure: Toward Photosafe Detection of Deep‐Seated Tumors

Cited by 29 publications

References 48 publications

A rapid and universal method for depth estimation of lesions in heterogeneous tissues via photosafe ratiometric transmission Raman spectroscopy

A rapid and universal method for depth estimation of lesions in heterogeneous tissues via photosafe ratiometric transmission Raman spectroscopy

Recent development of surface-enhanced Raman scattering for biosensing

Non‐Invasive Detection, Precise Localization, and Perioperative Navigation of In Vivo Deep Lesions Using Transmission Raman Spectroscopy

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