In vivo test of Litopenaeus vannamei infected by Vibrio using Moringa oleifera leaf extract

Abstract

This study aimed to determine the optimal concentration of Moringa oleifera leaf extract as an antibacterial agent on Litopenaeus vannamei larvae that are infected by Vibrio sp.. It was conducted in 15 March - 15 April 2019 at PT. Global Gain Superior Seeds, Pantai Cermin, North Sumatera. The research used a completely randomized design (CRD) method with five treatments and four replications. The shrimps infected by Vibrio sp. were immersed into M. oleifera leaf extract in various concentration of (A) 0 ppm, (B) 400 ppm, (C) 600 ppm, (D) 800 ppm, and (E) 1000 ppm for ±15 minutes. The size of shrimp used was post-larvae 8 (length of 3.5-5 mm/ind). The best performance results of vannamei shrimps were obtained in treatment E (1000 ppm), namely length growth of 16.75 ± 1.16 cm, weight growth of 0.92 ± 0.89 g and survival rate of 91±7.87%.Keywords:Moringa oleiferaVibrio sp.Immersion

Moringa oleifera Leaves’ Extract Enhances Nonspecific Immune Responses, Resistance against Vibrio alginolyticus, and Growth in Whiteleg Shrimp (Penaeus vannamei)

“…Moringa leave powder supplementation at 1.5 to 5% in diet effectively enhanced the immunity and controlled infection in nile tilapia ( Oreochromis niloticus ) [ 15 ], and at 20% in diet it enhanced the antioxidant activity and immune parameters of rainbow trout (Oncorhyncus mykiss) [ 16 ]; moreover, at 15% in diet it increased the growth and immune responses of grass carp (Ctenopharyngodon idella) [ 17 ]. In aqueous extract form, at 600 ppm, it induced antioxidant and antigenotoxic effects in nile tilapia [ 18 ], and at 1000 ppm it increased the resistance of whiteleg shrimp against V. harveyi [ 19 ]; moreover, mixing at 20 mL per kg diet improved the immunity and stress resistance of common carp ( Cyprinus carpio ) [ 20 ]. Despite its numerous advantages, moringa also has toxicological properties, which showed a dose-response relationship in some species [ 16 , 21 , 22 , 23 ].…”

Effect of herbal leaf extract on Oreochromis niloticus immunity infected with Streptococcus agalactiae

“…Then soak (maceration) with 2 liters of ethanol solution and soak for 24 hours. After 24 hours, the solution was filtered using a filter and evaporated using an evaporator [6].…”

“…Therefore, alternative medicine is needed using natural plants as herbal medicine, including Moringa oleivera leaves, Calotropis gigantea leaves, and Cassia alata L. leaves. This natural plant is known to contain antibacterial compounds to treat fish and shrimp infected with bacterial diseases [6,7,8].…”