In vivo Untersuchungen zur Neovascularisierung und Integration des “Soft Tissue Patch”

Walter, P.; Menger, Michael D.; Wolf, Beate; Meßmer, K.

doi:10.1007/978-3-642-75576-7_11

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…In the present study the composite of ovine collagen and polyester mesh showed early neovascularization and tight incorporation within the perigraft as is known for noncoated dacron grafts (26). These results are in agreement with findings on neovascularization of biosynthetic softtissue patches, which also consist of ovine collagen and polyester mesh (37). In addition, Ketharnathan and Christie (21) found in glutaraldehyde-tanned ovine collagen conduits in dogs a neoadventitia containing collagen and blood vessels as well as neointima lined by endothelium.…”

Section: Discussionsupporting

confidence: 91%

“…In addition, neovascularization is known to improve incorporation of the graft into the perigrafl tissue (26,29) and stabilization provided to the outer capsule appears important for the inner wall healing (34). Histological examinations (6,7,8,22,24,33) as well as in vivo studies from our laboratory (26,27,37) could demonstrate that porous graft materials are neovascularized better than impervious materials.…”

Section: Discussionmentioning

confidence: 94%

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In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

Menger¹,

Hammersen²,

Meßmer³

1992

Thorac cardiovasc Surg

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Neovascularization plays a major role in prosthetic vascular graft healing. New developments in manufacturing biomaterials have encouraged the use of biological and biosynthetic materials for both arterial replacement and bypass procedures. We have analyzed in vivo the process of neovascularization and incorporation of biolized bovine carotid artery (Solco P), a biological material, and the composite of ovine collagen and polyester mesh (Omniflow), a biosynthetic material. The synthetic fabric polytetrafluorethylene (e-PTFE), which is widely used in cardiovascular surgery, served as control. Using the dorsal skinfold chamber of the Syrian golden hamster as site for implantation (n = 15), angiogenesis and neovascularization were analyzed quantitatively by means of intravital fluorescence microscopy. In each chamber a piece (approximately 1 mm2) of each of the three vascular grafts was implanted. Five days after implantation neovascularization was ascertained in 90% of the Omniflow grafts, while only 40% of the PTFE and 20% of the Solco P implants revealed new ingrowing microvessels. On day 10 the density of newly formed microvessels was significantly higher (p less than 0.01) in Omniflow grafts (263.5 +/- 24.1 cm-1) as compared to PTFE (134.2 +/- 20.8 cm-1) and Solco P (153.2 +/- 32.0 cm-1). In addition, the biosynthetic composite revealed a larger extension of neovascularization into the perigraft tissue, and 12 days after implantation these grafts were most tightly incorporated.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

Menger¹,

Hammersen²,

Meßmer³

1992

Thorac cardiovasc Surg

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“…In the following years the microscopic approaches to the heart [10], liver [17] and pancreas [14], pancreatic islets [1], intestine [18], skin [23], muscle [4], nerve [13], and periostal tissue [24] were introduced, and served mainly for experimental analyses in perioperative pathophysiology, shock, sepsis, and transplantation (Table 1). Moreover, the technique was also shown to enable for the study of angiogenesis and vascularization of biosynthetic tissues [31] and tumors [6].…”

Section: Intravital Microscopy In Surgical Researchmentioning

confidence: 99%

The use of intravital microscopy in surgical research

Vollmar

Menger²

1998

Langenbeck's Archives of Surgery

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Recent developments in intravital microscopy make this technique an attractive approach to studying microvascular, cellular, and molecular mechanisms of distinct surgical diseases. We investigated the value of this technique in surgical research laboratories by analyzing the studies presented during the past 26 years (1972-1997) at the Surgical Forum of the Annual Congress of the German Society of Surgery. From a total of 2279 papers 188 contributions (8.3%) presented data which derived from the analysis of the microcirculation using techniques, such as H2 and 133Xe clearance, autoradiography, thermodiffusion, laser Doppler fluxmetry, laser speckle, radioactive and fluorescent microspheres, polarographic oximetry, and intravital microscopy. There were 72 presentations (3.2% of all contributions) reporting the use of intravital microscopy, thus reflecting 38.3% of all microcirculatory analyses. Although these numbers may be considered quite small, analysis over time revealed a significant (P<0.05) increase in the number of microcirculatory studies (11.4%) and in particular of those using intravital microscopy (6.3%) in the 1990s when compared to the 1970s (5.3%; 0.1%) and 80th (7.1 %; 1.3%). In 1997, 27 of 165 contributions (16.4%) included microcirculatory analyses, and 18 of the 165 contributions (10.9%) reported results analyzed by intravital microscopy. Thus our analysis reflects an increasing interest of surgical researchers to study in vivo the microcirculation, and by doing so to use intravital microscopy for the elucidation of mechanisms of surgical disease.

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In vivo Untersuchungen zur Neovascularisierung und Integration des “Soft Tissue Patch”

Cited by 2 publications

References 5 publications

In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

In Vivo Assessment of Neovascularization and Incorporation of Prosthetic Vascular Biografts

The use of intravital microscopy in surgical research

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