In vivo visualization of eosinophil secretion in eosinophilic granulomatosis with polyangiitis: An ultrastructural study

Koike, Haruki; Nishi, Ryoji; Furukawa, Soma; Mouri, Naohiro; Fukami, Yasuyuki; Iijima, Masahiro; Katsuno, Masahisa

doi:10.1016/j.alit.2022.02.009

Cited by 17 publications

(7 citation statements)

References 41 publications

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“…For electron microscopy, epoxy‐resin‐embedded specimens were cut into 70‐nm‐thick ultrathin transverse sections using an ultramicrotome. Sections were placed on a square 100‐mesh copper grid of 250‐μm pitch and 200‐μm hole, stained with uranyl acetate and lead citrate, and viewed under a transmission electron microscopy (JEM‐1400EX; JEOL Ltd. Tokyo, Japan) 8 . The second half of the specimen was fixed in 10% formalin and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

“…Sections were placed on a square 100-mesh copper grid of 250-μm pitch and 200-μm hole, stained with uranyl acetate and lead citrate, and viewed under a transmission electron microscopy (JEM-1400EX; JEOL Ltd. Tokyo, Japan). 8 The second half of the specimen was fixed in 10% formalin and embedded in paraffin. Tissues were sectioned using routine methods and stained with hematoxylin and eosin as well as with Masson trichrome.…”

Section: Patientsmentioning

confidence: 99%

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Early ultrastructural lesions of anti‐neutrophil cytoplasmic antibody‐ versus complement‐associated vasculitis

et al. 2022

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This study aims to describe electron microscopic findings of vasculitis associated with anti-neutrophil cytoplasmic antibody (ANCA) and complement. Sural nerve biopsy specimens were obtained from 10 patients with microscopic polyangiitis (MPA), a representative ANCAassociated vasculitis, and six patients with nonsystemic vasculitic neuropathy (NSVN), who were negative for ANCA but positive for complement deposition. In patients with MPA, attachment of neutrophils to epineurial vascular endothelial cells, migration of neutrophils to the extravascular space via the penetration of the endothelial layer, and release of neutrophil components to the extracellular space were observed. Such neutrophilassociated lesions were not observed in patients with NSVN. Nonetheless, morphological changes in epineurial vascular endothelial cells, such as increases in cytoplasmic organelles and cytoplasmic protrusions into the vascular lumen, were observed in patients with NSVN. Since these findings were observed where light microscopy-based findings suggestive of vasculitis (e.g., the disruption of vascular structures and fibrinoid necrosis) were absent, they were considered early lesions that preceded the formation of the so-called necrotizing vasculitis. In conclusion, this study enabled the visualization of distinctive early ultrastructural lesions associated with ANCA and complement. Further studies are needed to elucidate the molecular basis of the induction of these fine structural changes, which will contribute to the development of targeted therapies based on specific mechanisms of vasculitis.

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Section: Methodsmentioning

confidence: 99%

Section: Patientsmentioning

confidence: 99%

Early ultrastructural lesions of anti‐neutrophil cytoplasmic antibody‐ versus complement‐associated vasculitis

et al. 2022

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“…EGPA is a systemic small-vessel vasculitis associated with asthma, eosinophilia, and neuropathy. Pathologically, EGPA is considered to be a triad consisting of necrotizing vasculitis, eosinophilic infiltration, and extravascular granuloma, and the presence of ANCAs is associated with the clinical and pathological features of eosinophilic granulomatosis ( 10 , 11 ). MPO–ANCA is often detected in the sera of patients with MPA and EGPA, while, PR3-ANCA is a useful marker for GPA.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Gender and 1-Year Mortality in ANCA-Associated Vasculitis Patients: A Single-Center Retrospective Analysis and Meta-Analysis

Zhu

Dong-xing

et al. 2022

Front. Med.

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ObjectiveThe relationship between gender and short-term prognosis of patients with anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV) is unclear, hence single-center retrospective analysis and meta-analysis were conducted to determine the relationship.MethodsInitially treated patients with AAV were retrospectively enrolled. Data of clinical manifestation, laboratory indicators, Birmingham vasculitis activity score (BVAS), therapeutic treatments, and the patients' situations within 1 year were recorded. First, we compared the basic characteristics between male and female patients. Second, the risk factors associated with a 1-year mortality rate of patients with AAV were evaluated. Finally, a meta-analysis was performed to explore the effect of gender on 1-year mortality in patients with AAV.ResultsThe study involved 84 patients with AAV, including 33 female and 51 male participants. In total, 14 people died (12 males and 2 females) and 70 survived in the 1st year. Statistical differences were noted in the age of onset, the course of the disease, WBC, HB, N, ESR, CRP, BUN, ALT and ALB, BVAS, and 1-year mortality rate between male and female participants. In male patients, elevated Scr, NLR, PLT, and RDW-CV were associated with poor AAV (P < 0.05) prognosis. The meta-analysis verified that male gender was an independent risk factor for the 1-year mortality of patients with AAV(OR = 1.54).ConclusionSignificant sex-specific differences were found in patients with AAV. Male patients contributed to 1.54-fold of 1-year mortality risk in patients with AAV by meta-analysis. More attention should be paid to the mortality risk of male patients with AAV in the early stage.

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“…Recent studies have provided valuable insights into the pathophysiology of EGPA and antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Notably, research by Haruki et al in 2022 visualized the dynamic behavior of eosinophils in EGPA, highlighting their role in the disease process [ 5 ]. They also elucidated the contribution of neutrophil-associated vascular damage in ANCA-associated vasculitis, further enriching our understanding of these complex conditions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

A Changing Anti-Neutrophil Cytoplasmic Antibody Profile in a Patient With a Diagnosis of Eosinophilic Granulomatosis With Polyangiitis

Jinno,

Kozu,

Hiranuma

et al. 2023

J Med Cases

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This report describes a hitherto unique case of eosinophilic granulomatosis with polyangiitis (EGPA), a subtype of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. The patient was an 81-year-old man whose clinical course involved notable changes in the ANCA profile, specifically a transition from positive proteinase 3 (PR3)-ANCA to myeloperoxidase (MPO)-ANCA, followed by simultaneous positivity for both. The patient’s medical history included bronchial asthma, allergic rhinitis, sinusitis, and multiple comorbidities. Despite being initially PR3-ANCA-positive, subsequent admissions demonstrated MPO-ANCA positivity along with eosinophilic manifestations, highlighting the complexity of diagnosis of EGPA. Diagnostic evaluation included imaging, serological markers, and clinical symptoms, which collectively supported the classification of EGPA. Notably, this case challenges the conventional diagnostic paradigms and emphasizes the evolving nature of ANCA profiles in vasculitis. The shift in ANCA profile prompted a reevaluation of the patient’s diagnosis and treatment strategy. This case underscores the importance of considering fluctuations in ANCA in patients with a diagnosis of EGPA, management decisions, and potential implications for disease progression. Further research is warranted to elucidate the mechanisms underlying changes in ANCA and their clinical significance in vasculitis.

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In vivo visualization of eosinophil secretion in eosinophilic granulomatosis with polyangiitis: An ultrastructural study

Cited by 17 publications

References 41 publications

Early ultrastructural lesions of anti‐neutrophil cytoplasmic antibody‐ versus complement‐associated vasculitis

Early ultrastructural lesions of anti‐neutrophil cytoplasmic antibody‐ versus complement‐associated vasculitis

Relationship Between Gender and 1-Year Mortality in ANCA-Associated Vasculitis Patients: A Single-Center Retrospective Analysis and Meta-Analysis

A Changing Anti-Neutrophil Cytoplasmic Antibody Profile in a Patient With a Diagnosis of Eosinophilic Granulomatosis With Polyangiitis

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