In VivoAddition of Telomeric Repeats to Foreign DNA Generates Extrachromosomal DNAs in the Taxol-Producing FungusPestalotiopsis microspora

Long, David M.; Smidansky, Eric D.; Archer, Amy J.; Strobel, Gary A.

doi:10.1006/fgbi.1998.1065

Cited by 40 publications

(22 citation statements)

References 27 publications

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“…Long et al (1998) have shown that under laboratory conditions it can take up heterologous DNA, add telomeric DNA, express heterologous DNA and can replicate independently of chromosomal DNA. Such genetic diversity would be useful to the species in nature, helping it adapt to a new plant by incorporating plant DNA into its own genome (Strobel et al 1996a;Li et al 1996).…”

Section: Novel Pestalotiopsis Biochemistrymentioning

confidence: 99%

Pestalotiopsis—morphology, phylogeny, biochemistry and diversity

et al. 2011

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The genus Pestalotiopsis has received considerable attention in recent years, not only because of its role as a plant pathogen but also as a commonly isolated endophyte which has been shown to produce a wide range of chemically novel diverse metabolites. Classification in the genus has been previously based on morphology, with conidial characters being considered as important in distinguishing species and closely related genera. In this review, Pestalotia, Pestalotiopsis and some related genera are evaluated; it is concluded that the large number of described species has resulted from introductions based on host association. We suspect that many of these are probably not good biological species. Recent molecular data have shown that conidial characters can be used to distinguish taxa; however, host association and geographical location is less informative.

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Section: Novel Pestalotiopsis Biochemistrymentioning

confidence: 99%

Pestalotiopsis—morphology, phylogeny, biochemistry and diversity

et al. 2011

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“…Unrelated fungi such as the basidiomycetous yeast Crytococcus neoformans, the dimorphic ascomycete Histoplasma capsulatum, the ®lamentous deuteromycete Fusarium oxysporum (Powell and Kistler 1990) and the taxol-producing ®lamentous fungus Pestalotiopsis microspora (Long et al 1998), can add telomeric repeats to transforming DNA for reasons unknown. Linear transformation vectors containing telomere consensus sequences were created in Fusarium oxysporum by fungal rearrangement of an integrating vector, and functioned with high ef®ciency as autonomously replicating vector in the plant pathogens Nectria haematococca and Cryphonectria parasitica, as well as in F. oxysporum (Powell and Kistler 1990).…”

Section: Fate Of Transforming Dnamentioning

confidence: 99%

Strategies for the transformation of filamentous fungi

2002

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“…Under laboratory conditions it can take up heterologous DNA, add telomeric DNA, express the heterologous DNA and replicate it independently of chromosomal DNA (Long et al, 1998). It has been suggested that comparable activity in this fungus may exist in nature, allowing it to readily adapt to a new plant by incorporating plant DNA into its own genome (Strobel et al, 1996a ;Li et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Induction of the sexual stage of Pestalotiopsis microspora, a taxol-producing fungus The GenBank accession numbers for the sequences determined in this work are: Pestalotiopsis microspora NE-32 18S rDNA, AF104356; Pestalosphaeria hansenii ATCC 48245 18S rDNA, AF242846.

et al. 2000

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Pestalotiopsis microspora, isolate NE-32, is an endophyte of the Himalayan yew (Taxus wallichiana) that produces taxol, an important chemotherapeutic drug used in the treatment of breast and ovarian cancers. Conditions were determined to induce the perfect stage (teleomorph) of this organism in the laboratory as a critical first step to study inheritance of taxol biosynthetic genes. The perfect stage of Pestalotiopsis microspora NE-32 forms in a period of 3-6 weeks on water agarose with dried yew needles at 16-20 SC with 12 h of light per day. Morphological analysis of the teleomorph and sequencing of the 18S rDNA indicates that Pestalosphaeria hansenii is the perfect stage of Pestalotiopsis microspora. Only certain plants (e.g. yews, some pines, pecan, oat and some barley cultivars) allow the production of perithecia. Exhaustive methylene chloride extraction of yew (Taxus cuspidata) needles removes their capacity to induce production of perithecia. The methylene chloride extract is able to induce formation of perithecia by strain NE-32 in a bioassay system utilizing the sterilized sheaths of the Cholla cactus (Opuntia bigelovii) spine, indicating that a chemical compound(s) in yew stimulates the formation of the perfect stage. This hydrophobic plant compound(s) has been designated the perithecial-stimulating factor (PSF). The data suggest that plant products may play a role in regulating the biology of endophytic microbes.

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In VivoAddition of Telomeric Repeats to Foreign DNA Generates Extrachromosomal DNAs in the Taxol-Producing FungusPestalotiopsis microspora

Cited by 40 publications

References 27 publications

Pestalotiopsis—morphology, phylogeny, biochemistry and diversity

Pestalotiopsis—morphology, phylogeny, biochemistry and diversity

Strategies for the transformation of filamentous fungi

Induction of the sexual stage of Pestalotiopsis microspora, a taxol-producing fungus The GenBank accession numbers for the sequences determined in this work are: Pestalotiopsis microspora NE-32 18S rDNA, AF104356; Pestalosphaeria hansenii ATCC 48245 18S rDNA, AF242846.

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