Inactivation and oxidative modification of Cu,Zn superoxide dismutase by stimulated neutrophils: The appearance of new catalytically active structures

Sharonov, Boris P.; Churilova, I. V.

doi:10.1016/0006-291x(92)92321-n

Cited by 29 publications

(9 citation statements)

References 19 publications

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“…These include SOD and CAT, which can detoxify O 2 − and H 2 O 2 , respectively (Fang et al 2002), and non-enzymatic components, such as glutathione, selenium and vitamins C and E (El-Bahr 2013). Antioxidant enzymes are prone to oxidative modification and inactivation due to alteration by ROS and/or induction by their chemical environment (Sharonov and Churilova 1992). In this study, it is particularly interesting that we observed an increase in antioxidant enzyme activities: even at maximum copper exposure the enzymatic activity of SOD and CAT remained slightly higher than in the control.…”

Section: Discussionsupporting

confidence: 46%

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Sun

Zhu

et al. 2014

Sci. Mar.

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Summary:The lethal concentration of water-borne copper in Chinese mitten crab Eriocheir sinensis larvae was tested by exposing the animals to 0, 0.1, 0.2, 0.3, 0.5 and 0.8 mg Cu L −1 at 20°C for 96 h. The 96-h median lethal concentration (LC 50 ) and its corresponding 95% confident interval was estimated on zoea 1 larvae and megalopa larvae, respectively. Acute dissolved copper toxicity was higher for zoea 1 larvae (0.16 mg L -1 ) than for megalopa larvae (0.21 mg L -1 ). The antioxidant status, metallothionein-I mRNA expression and physiological response of the crab to copper toxicity was further investigated by exposing the megalopa larvae to 0, 0.08 and 0.16 mg Cu L −1 for 96 h. The superoxide dismutase activity, catalase activity, glutathione S-transferase (GST) activity and lipid peroxidation content of megalopa larvae increased concomitantly with the exposure time and copper concentration. MT-I mRNA expression levels were positively correlated with both the concentration and duration of copper exposure. The oxygen consumption and respiratory quotient of megalopa larvae in response to 0.16 mg L -1 copper were significantly higher than those in the control group after 96 h of exposure (P<0.05). The results of this study highlight the potential effects of copper as a common stressor in E. sinensis larvae. MT-I and GST appear to be suitable biomarkers of environmental copper exposure stress in E. sinensis larvae.Keywords: Eriocheir sinensis; copper toxicity; physiological; larvae; antioxidant enzyme; metallothionein.Efectos del cobre disuelto sobre la supervivencia, estado antioxidante, expresión de la metalotionina-I mRNA y la respuesta fisiológica de las larvas del cangrejo de Shangai, Eriocheir sinensis (Decapoda: Brachyura) Resumen: La concentración letal de cobre disuelto se determina mediante la exposición de larvas del cangrejo de Shangai (Eriocheir sinensis) a dosis de 0, 0.1, 0.2, 0.3, 0.5 y 0.8 mg Cu L -1 , a 20°C durante 96 h. La concentración letal media (LC 50 ) y su correspondiente intervalo de confianza del 95% fueron estimados en los estadios larvales zoea I y megalopa. La toxicidad aguda del cobre disuelto fue mayor sobre larvas de zoea I (0.16 mg L -1 ) que sobre las de megalopa (0.21 mg L -1 ). El estado antioxidante, la expresión del mRNA de la metalotionina-I y la respuesta fisiológica del cangrejo a la toxicidad del cobre fueron posteriormente investigados exponiendo las larvas de megalopa a dosis de 0, 0.08 y 0.16 mg Cu L -1 durante 96 h. La actividad de la superóxido dismutasa (SOD), de la catalasa (CAT), de la glutatión-S-transferasa (GST) y la peroxidación lipídica de las larvas de megalopa se incrementó en relación con el tiempo de exposición y la concentración de cobre. Los niveles de expresión de MT-1 mRNA se correlacionaron positivamente con la concentración de cobre y el tiempo de exposición. El consumo de oxígeno y la tasa respiratoria de las larvas de megalopa fueron significativamente más elevadas que las del grupo control (P<0.05) en respuesta a dosis de 0.16 mg Cu L -1 , du...

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Section: Discussionsupporting

confidence: 46%

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Sun

Zhu

et al. 2014

Sci. Mar.

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“…Previous reports have shown that in the liver of rats treated once with CCl 4 the activity of Cu,Zn-SOD present in the cytosol, but not the activity of Mn-SOD present in the mitochondria, decreases with hepatic injury development (Ohta et al, 2003(Ohta et al, , 2004. Cu,Zn-SOD is known to be inactivated by HOCl and by the MPO-H 2 O 2 -Cl − system (Aruoma and Halliwell, 1987;Sharonov and Churilova, 1992;Auchère and Capeillère-Blandin, 2002). Escobar et al (1996) have shown that alkyl-peroxyl radicals generated by 2,2′-azobis(2-amidinopropane), which is used as a source of radicals for lipid peroxidation, inactivate Cu,Zn-SOD in vitro.…”

Section: Discussionmentioning

confidence: 98%

Preventive effect of neutropenia on carbon tetrachloride-induced hepatotoxicity in rats

et al. 2006

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The preventive effect of neutropenia on carbon tetrachloride (CCl4)-induced hepatotoxicity was examined in rats. In rats treated once with CCl4 (1 ml kg(-1), i.p.), the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), indices of liver cell damage, and the hepatic activity of myeloperoxidase (MPO), an index of tissue neutrophil infiltration, increased at 6 h after the intoxication and further increased at 24 h. The liver of CCl4 -treated rats showed an increase in the concentration of thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation, and decreases in superoxide dismutase (SOD) activity and reduced glutathione (GSH) concentration at 6 h after the intoxication followed by a further increase in TBARS concentration and further decreases in SOD activity and GSH concentration at 24 h with increased xanthine oxidase (XO) activity at 24 h. Neutropenic treatment with anti-rat neutrophil antiserum (2 ml kg(-1), i.p.) at 0.5 h after CCl4 intoxication attenuated the increases in serum ALT and AST activities and hepatic MPO activity and TBARS concentration and the decreases in hepatic SOD activity and GSH concentration found at 6 and 24 h after CCl4 intoxication and the increase in hepatic XO activity found at 24 h after the intoxication. This neutropenia reduced the necrotic and degenerative changes with inflammatory cell infiltration in the liver cell of CCl4 -treated rats. These results indicate that neutropenia prevents CCl4 -induced hepatotoxicity in rats by attenuating the disruption of hepatic reactive oxygen species metabolism mediated by neutrophils accumulating in the liver tissue.

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“…Cu,Zn-SOD catalyses the formation of the hydroxyl radical from hydrogen peroxide and can be oxidatively modified, yielding a more acidic protein (60,61). The oxidative origin of the new SOD bands was confirmed by incubation of cellfree extracts with different systems generating reactive oxygen species (38,57,61,62).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and detoxifying enzymes in the liver and kidney of pheasants after intoxication by herbicides MCPA and ANITEN

Holovská

Lenártová

Rosíval

et al. 1998

J. Biochem. Mol. Toxicol.

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Inactivation and oxidative modification of Cu,Zn superoxide dismutase by stimulated neutrophils: The appearance of new catalytically active structures

Cited by 29 publications

References 19 publications

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, <em>Eriocheir sinensis</em> (Decapoda: Brachyura) larvae

Preventive effect of neutropenia on carbon tetrachloride-induced hepatotoxicity in rats

Antioxidant and detoxifying enzymes in the liver and kidney of pheasants after intoxication by herbicides MCPA and ANITEN

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