Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces

Doerrbecker, Juliane; Friesland, Martina; Ciesek, Sandra; Erichsen, Thomas; Mateu‐Gelabert, Pedro; Steinmann, Jörg; Steinmann, J.; Pietschmann, Thomas; Steinmann, Eike

doi:10.1093/infdis/jir535

Cited by 95 publications

(83 citation statements)

References 38 publications

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“…Interestingly, the protective effect of organic load for chlorine and peracetic acid inactivation was not observed for another flavivirus (WNV) examined in this study. In this regard, our results with WNV were similar to those described previously for the flavivirus hepatitis C [12]. Doerrbecker et al [12] dried HCV onto stainless steel coupons in the absence or presence of 50% human serum as organic load, and then subjected the virus to a series of alcohols and disinfectant products, including quaternary ammonium compounds and peroxides.…”

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavisupporting

confidence: 86%

“…In this regard, our results with WNV were similar to those described previously for the flavivirus hepatitis C [12]. Doerrbecker et al [12] dried HCV onto stainless steel coupons in the absence or presence of 50% human serum as organic load, and then subjected the virus to a series of alcohols and disinfectant products, including quaternary ammonium compounds and peroxides. The data indicated that the human serum did not confer resistance to alcohols and other biocides tested for HCV.…”

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavisupporting

confidence: 86%

“…Similar results were obtained for two additional flaviviruses examined, BVDV and WNV. Isopropyl alcohol was also found to be very effective for inactivating hepatitis C virus when dried onto steel coupons in the presence or absence of human serum [12]. In either case, concentrations of isopropyl alcohol as low as 40% reduced the titer of hepatitis C virus >4 log 10 within 5 min contact time.…”

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavimentioning

confidence: 98%

“…Müller et al did not evaluate the impact of organic load at the time of drying of the virus on the environmental stability. Doerrbecker et al [12] reported that the survival of another flavivirus (hepatitis C virus, HCV; genus Hepacivirus) was actually reduced by approximately 1.0-1.5 log 10 when dried in the presence of an organic load (human serum). It remains to be confirmed whether the survivability of HCV is positively or negatively impacted by blood.…”

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavimentioning

confidence: 99%

See 3 more Smart Citations

Inactivation and Disinfection of Zika Virus on a Nonporous Surface

Wilde¹,

Chen²,

Kapes³

et al. 2016

J Microb Biochem Technol

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Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavisupporting

confidence: 86%

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavisupporting

confidence: 86%

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavimentioning

confidence: 98%

Section: Comparison Of Inactivation Efficacy For Zikv Vs Other Flavimentioning

confidence: 99%

See 2 more Smart Citations

Inactivation and Disinfection of Zika Virus on a Nonporous Surface

Wilde¹,

Chen²,

Kapes³

et al. 2016

J Microb Biochem Technol

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“…Different studies have recently evaluated the environmental stability of HCV and its susceptibility to chemical biocides in quantitative suspension assays (15)(16)(17)(18)(19) or on dried surfaces (20,21). However, virus inactivation mechanisms of these and other procedures and the question of which parts of the virus particles are specifically disrupted have not been addressed.…”

mentioning

confidence: 99%

Mechanisms of Methods for Hepatitis C Virus Inactivation

Pfaender

Brinkmann

Todt

et al. 2015

Appl Environ Microbiol

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Virus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and the influence of chemical disinfectants, heat, and UV treatment on HCV was analyzed by the determination of infectious particles in a limiting-dilution assay, by quantitative reverse transcription-PCR, by core enzyme-linked immunosorbent assay, and by proteolytic protection assay. All different inactivation methods resulted in a loss of HCV infectivity by targeting different parts of the virus particle. Alcohols such as ethanol and 2-propanol did not affect the viral RNA genome integrity but disrupted the viral envelope membrane in a capsid protection assay. Heat and UV treatment of HCV particles resulted in direct damage of the viral genome since transfection of viral particle-associated RNA into permissive cells did not initiate RNA replication. In addition, heat incubation at 80°C disrupted the HCV envelope, rendering the viral capsid susceptible to proteolytic digest. This study demonstrated the molecular processes of viral inactivation of an enveloped virus and should facilitate the development of effective disinfection strategies in infection control not only against HCV but also against other enveloped viruses.

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Association of tattooing and hepatitis C virus infection: A multicenter case-control study

et al. 2013

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Although injection drug use (IDU) and blood transfusions prior to 1992 are well-accepted risk factors for hepatitis C virus (HCV) infection, many studies that evaluated tattooing as a risk factor for HCV infection did not control for a history of IDU or transfusion prior to 1992. In this large, multicenter, case-control study, we analyzed demographic and HCV risk factor exposure history data from 3,871 patients, including 1,930 with chronic HCV infection (HCV RNA-positive) and 1,941 HCV-negative (HCV antibody-negative) controls. Crude and fully adjusted odds ratios (ORs) of tattoo exposure by multivariate logistic regression in HCV-infected versus controls were determined. As expected, IDU (65.9% versus 17.8%; P < 0.001), blood transfusion prior to 1992 (22.3% versus 11.1%; P < 0.001), and history of having one or more tattoos (OR, 3.81; 95% CI, 3.23-4.49; P < 0.001) were more common in HCV-infected patients than in control subjects. After excluding all patients with a history of ever injecting drugs and those who had a blood transfusion prior to 1992, a total of 1,886 subjects remained for analysis (465 HCV-positive patients and 1,421 controls). Among these individuals without traditional risk factors, HCV-positive patients remained significantly more likely to have a history of one or more tattoos after adjustment for age, sex, and race/ethnicity (OR, 5.17; 95% CI, 3.75-7.11; P < 0.001). Conclusion: Tattooing is associated with HCV infection, even among those without traditional HCV risk factors such as IDU and blood transfusion prior to 1992. (HEPATOLOGY 2013;57:2117-2123 As new and better medications for the treatment of HCV become available, [13][14][15] measures to increase detection rates and engagement in care are paramount. In the last decade, an expanding body of research has emerged, evaluating both traditional and nontraditional risk factors for HCV infection in an effort to increase the yield from costly yet potentially life-saving screening efforts. 7,[16][17][18][19] Most recently, the US Department of Health and Human Services issued an action plan for the prevention, care, and treatment of viral hepatitis, setting goals to increase the proportion of persons who are aware of their HCV infection from 45% to 66%, and to reduce the number of new cases of HCV infection by 25%. 20In contrast to the overwhelming evidence implicating IDU in HCV acquisition, the association between HCV transmission and other suspected risk factors such as tattooing is more controversial. Although some studies have demonstrated an association between tattoos and HCV infection, others have not.21 Prior studies that examined tattooing behavior and HCV

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Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces

Abstract: These findings are important for assessment of HCV transmission risks and should facilitate the definition of stringent public health interventions to prevent HCV infections.

Cited by 95 publications

References 38 publications

Inactivation and Disinfection of Zika Virus on a Nonporous Surface

Inactivation and Disinfection of Zika Virus on a Nonporous Surface

Mechanisms of Methods for Hepatitis C Virus Inactivation

Association of tattooing and hepatitis C virus infection: A multicenter case-control study

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