Inactivation effect of an 18-T pulsed magnetic field combined with other technologies on Escherichia coli

Martı́n, M.Fernanda San; Harte, Federico; Lelieveld, Huub; Barbosa‐Cánovas, Gustavo V.; Swanson, Barry G.

doi:10.1016/s1466-8564(01)00049-2

Cited by 35 publications

(7 citation statements)

References 25 publications

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“…Despite the fact that the time and intensity dependent MF effects on the proliferative response of cells, similar in the case of all the three mitogens, cannot be explained on the basis of the present experiment, our findings are in agreement with several reports showing a lack of effect of the MF of higher intensity [Sakurai et al, 1999;San Martin et al, 2001] and some influence of a weaker MF (mT and mT range) on cell proliferation [Kwee and Raskmark, 1995;Stra s sák et al, 2002].…”

Section: Discussionsupporting

confidence: 90%

Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation

Román¹,

Zyss²,

Nalepa³

2005

Bioelectromagnetics

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We aimed to find out how the exposure of isolated lymphocytes to a pulsed magnetic field (MF) affected their in vitro proliferative response to mitogenic stimulation. Cells were exposed to MF of various intensities (0.3, 0.6, and 1.2 T) at a constant frequency of 30 Hz, for a period of 60, 180, and 330 s. Then, the proliferative response of splenocytes was induced by optimal concentrations of concanavalin A (Con A; mitogenic toward T cells), bacterial lipopolysaccharide (LPS; mitogenic toward B cells), or pokeweed mitogen (PWM; mitogenic toward both populations). We found that the exposure of lymphocytes to the MF profoundly inhibited their proliferative response to mitogens. The suppressive action of the MF on B and T cell proliferation was intensified when a cooperative response of those two lymphocyte populations was simultaneously induced by PWM. The inhibitory effect of MF depended on the exposure time and MF intensity. Prolonged exposure and/or a stronger intensity of the MF weakened its inhibitory influence on the response of lymphocyte to mitogenic stimulation. The data show that an exposure to MF may influence the activity of lymphocytes in their response to mitogenic stimuli.

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Section: Discussionsupporting

confidence: 90%

Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation

Román¹,

Zyss²,

Nalepa³

2005

Bioelectromagnetics

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“…As far as the magnetic field effect is concerned, the effect of magnetic field on the bio‐functionalities at cellular level of living organisms has been significantly studied,14–16 but the exact mechanism to influence the morphological changes, growth kinetics, and adhesion behavior of bacteria is still not clear. The exposure to pulse magnetic field,17 low frequency electromagnetic field (ELF),18, 19 high intensity (>1 tesla) homogeneous and inhomogeneous magnetic field,20 static magnetic field (SMF)21–23 for different timeframe and field intensity have proved its effect on the different cellular functionalities and physiological phenomena. The external oscillating magnetic field is supposed to induce forced vibrations on all the free ions on the surface of cellular plasma membrane, which is strong enough to disorder the electrochemical balance of the cell as well as necessary biological functions 16.…”

Section: Introductionmentioning

confidence: 99%

Moderate intensity static magnetic field has bactericidal effect on E. coli and S. epidermidis on sintered hydroxyapatite

2012

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The application of electromagnetic field in the context of bacteria associated infections on biomaterial surfaces has not been extensively explored. In this work, we applied a moderate intensity static magnetic field (100 mT) to understand the adhesion and growth behavior of both gram positive (S. epidermidis) and gram negative bacteria (E. coli) and also to investigate bactericidal/bacteriostatic property of the applied electromagnetic field. An in-house built magnetometer was used to apply static homogeneous magnetic field during a planned set of in vitro experiments. Both the sintered hydroxyapatite (HA) and the control samples seeded with bacteria were exposed to the magnetic field (100 mT) for different timescale during their log phase growth. Quantitative analysis of the SEM images confirms the effect of electromagnetic field on suppressing bacterial growth. Furthermore, cell integrity and inner membrane permeabilization assays were performed to understand the origin of such effect. The results of these assays were statistically analyzed to reveal the bactericidal effect of magnetic field, indicating cell membrane damage. Under the investigated culture conditions, the bactericidal effect was found to be less effective for S. Epidermidis than E. coli.

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“…2006, in press) and increasing product homogeneity and energy savings (Crosby 1982). Thermosonic and manosonic treatments were particularly effective in reducing Escherichia coli (San Martín et al. 2001), thermoduric streptococci (Ordoñez et al.…”

Section: Introductionmentioning

confidence: 99%

COMBINED EFFECT OF OSMOTIC PRESSURE AND SONICATION ON THE REDUCTION OF SALMONELLA SPP. IN CONCENTRATED ORANGE JUICE

Wong

Pérez

Vaillant

2008

Journal of Food Safety

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The effect of osmotic pressure alone or combined with the application of sonication on the reduction of Salmonella spp. in concentrated orange juice was evaluated. Frozen concentrated orange juice (12.6 MPa, pH = 3.2), a neutral sugar solution (9.2 MPa, pH = 6.6) and an acid sugar solution (8.8 MPa, pH = 3.2) were inoculated with Salmonella spp. (6-7 log cfu/mL). Reductions were measured after different storage times with or without previous sonication treatment of 1 h . No significant osmotic shock was observed. Reductions appeared to increase over storage time in high osmotic environments. Reductions were also significantly higher for sonicated samples when compared with nonsonicated samples. The highest reduction (7.21 log cfu/mL) was found for concentrated orange juice sonicated during 60 min and stored for 168 h. Combination of sonication and osmotic evaporation (osmosonication) represents a promising new technology that could be designed to athermally produce safe, concentrated fruit juices. PRACTICAL APPLICATIONSThe results derived from this research indicate that combining sonication with osmotic pressure during storage of concentrated orange juice provides a * Corresponding

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Inactivation effect of an 18-T pulsed magnetic field combined with other technologies on Escherichia coli

Cited by 35 publications

References 25 publications

Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation

Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation

Moderate intensity static magnetic field has bactericidal effect on E. coli and S. epidermidis on sintered hydroxyapatite

COMBINED EFFECT OF OSMOTIC PRESSURE AND SONICATION ON THE REDUCTION OF SALMONELLA SPP. IN CONCENTRATED ORANGE JUICE

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