Inactivation of a Peroxiredoxin by Hydrogen Peroxide Is Critical for Thioredoxin-Mediated Repair of Oxidized Proteins and Cell Survival

Day, Alison M.; Brown, Jonathon D.; Taylor, Sarah R.; Rand, Jonathan D.; Morgan, Brian A.; Veal, Elizabeth A.

doi:10.1016/j.molcel.2011.11.027

Cited by 188 publications

(205 citation statements)

References 40 publications

(78 reference statements)

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“…Interestingly, the 2-Cys Prx pool did not go through major redox state changes as the ACHT1 pool did during illumination of dark-adapted plants. These findings, which are reminiscent of the Yap1 oxidative sensing pathway (Delaunay et al, 2002) or of the Trx-Prx Tpx1 pathway in Schizosaccharomyces pombe (Day et al, 2012) and not of the circadian rhythmic biomarker 2-Cys Prxs , indicate that ACHT1 redox state changes are used for regulation. Conceivably, the additional proteins trapped in intermolecular disulfide complexes with ACHT1 ( Figure 4) could be ACHT1 targets regulated by ACHT1 redox state.…”

Section: Discussionmentioning

confidence: 93%

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

et al. 2012

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The transition from dark to light involves marked changes in the redox reactions of photosynthetic electron transport and in chloroplast stromal enzyme activity even under mild light and growth conditions. Thus, it is not surprising that redox regulation is used to dynamically adjust and coordinate the stromal and thylakoid compartments. While oxidation of regulatory proteins is necessary for the regulation, the identity and the mechanism of action of the oxidizing pathway are still unresolved. Here, we studied the oxidation of a thylakoid-associated atypical thioredoxin-type protein, ACHT1, in the Arabidopsis thaliana chloroplast. We found that after a brief period of net reduction in plants illuminated with moderate light intensity, a significant oxidation reaction of ACHT1 arises and counterbalances its reduction. Interestingly, ACHT1 oxidation is driven by 2-Cys peroxiredoxin (Prx), which in turn eliminates peroxides. The ACHT1 and 2-Cys Prx reaction characteristics in plants further indicated that ACHT1 oxidation is linked with changes in the photosynthetic production of peroxides. Our findings that plants with altered redox poise of the ACHT1 and 2-Cys Prx pathway show higher nonphotochemical quenching and lower photosynthetic electron transport infer a feedback regulatory role for this pathway.

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Section: Discussionmentioning

confidence: 93%

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

et al. 2012

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“…In particular, HP induces the formation of a transient disulphide complex between Tpx1 (Cys-48) and Sty1 (Cys-35) (Figure 4) (Veal et al, 2004). Additional redoxsensitive cysteine(s) present in the Sty1 protein or the reduced form of the thioredoxin Trx1 could further control the activation of Sty1 downstream of the SAPK pathway (Day et al, 2012;Veal et al, 2004).…”

Section: Activation Of Sty1 Results In Redirection Of Gene Expressionmentioning

confidence: 99%

Oxidative stress response pathways: Fission yeast as archetype

Papadakis¹,

Workman²

2015

Critical Reviews in Microbiology

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Schizosaccharomyces pombe is a popular model eukaryotic organism to study diverse aspects of mammalian biology, including responses to cellular stress triggered by redox imbalances within its compartments. The review considers the current knowledge on the signaling pathways that govern the transcriptional response of fission yeast cells to elevated levels of hydrogen peroxide. Particular attention is paid to the mechanisms that yeast cells employ to promote cell survival in conditions of intermediate and acute oxidative stress. The role of the Sty1/Spc1/Phh1 mitogen-activated protein kinase in regulating gene expression at multiple levels is discussed in detail.

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“…However, a number of these isoforms can be inactivated, mediated by the oxidation of the catalytic site Cys to Cys‐sulfinic acid (SO 2 H) by high levels of H 2 O 2 . PRXs that have formed SO 2 H acids can be reduced by sulfiredoxin, and it has been proposed that this initial inactivation by its substrate plays a cellular signalling role by a ‘floodgate mechanism’ 148. It is particularly interesting that there are a number of protein families and isoforms within those families that have either peroxidase activity (GPXs, PRXs and CAT) or regulatory proteins that are sensitive to oxidation (Keap1, aconitase and PTP1B).…”

Section: Regulatory Reactive Oxygen and Nitrogen Species Enzymes Exprmentioning

confidence: 99%

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

Sakellariou

Lightfoot

Earl

et al. 2017

J cachexia sarcopenia muscle

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Skeletal muscle is a major site of metabolic activity and is the most abundant tissue in the human body. Age‐related muscle atrophy (sarcopenia) and weakness, characterized by progressive loss of lean muscle mass and function, is a major contributor to morbidity and has a profound effect on the quality of life of older people. With a continuously growing older population (estimated 2 billion of people aged >60 by 2050), demand for medical and social care due to functional deficits, associated with neuromuscular ageing, will inevitably increase. Despite the importance of this ‘epidemic’ problem, the primary biochemical and molecular mechanisms underlying age‐related deficits in neuromuscular integrity and function have not been fully determined. Skeletal muscle generates reactive oxygen and nitrogen species (RONS) from a variety of subcellular sources, and age‐associated oxidative damage has been suggested to be a major factor contributing to the initiation and progression of muscle atrophy inherent with ageing. RONS can modulate a variety of intracellular signal transduction processes, and disruption of these events over time due to altered redox control has been proposed as an underlying mechanism of ageing. The role of oxidants in ageing has been extensively examined in different model organisms that have undergone genetic manipulations with inconsistent findings. Transgenic and knockout rodent studies have provided insight into the function of RONS regulatory systems in neuromuscular ageing. This review summarizes almost 30 years of research in the field of redox homeostasis and muscle ageing, providing a detailed discussion of the experimental approaches that have been undertaken in murine models to examine the role of redox regulation in age‐related muscle atrophy and weakness.

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Inactivation of a Peroxiredoxin by Hydrogen Peroxide Is Critical for Thioredoxin-Mediated Repair of Oxidized Proteins and Cell Survival

Cited by 188 publications

References 40 publications

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

Oxidative stress response pathways: Fission yeast as archetype

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

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