Inactivation of Candida albicans Following Exposure to 624-Nanometer Light from a Supraluminous Diode Array

Guffey, J. Stephen; Payne, William; James, Leslie N.; Qian, Zhuoyuan; Dodson, Carly

doi:10.1097/01.asw.0000443268.25288.63

Cited by 13 publications

(18 citation statements)

References 14 publications

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“…Guffey et al reported that aBL at 405 nm was effective in inactivating Mycobacterium smegmatis , which is a non-pathogenic model system for the pathogenic Mycobacterium tuberculosis (Guffey et al, 2013a). All aBL exposures from 60 to 250 J/cm 2 produced a significant inactivation rate with the highest exposure (250 J/cm 2 ) demonstrating 100% inactivation.…”

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

227

192

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As an innovative non-antibiotic approach, antimicrobial blue light in the spectrum of 400–470 nm has demonstrated its intrinsic antimicrobial properties resulting from the presence of endogenous photosensitizing chromophores in pathogenic microbes and, subsequently, its promise as a counteracter of antibiotic resistance. Since we published our last review of antimicrobial blue light in 2012, there have been a substantial number of new studies reported in this area. Here we provide an updated overview of the findings from the new studies over the past 5 years, including the efficacy of antimicrobial blue light inactivation of different microbes, its mechanism of action, synergism of antimicrobial blue light with other angents, its effect on host cells and tissues, the potential development of resistance to antimicrobial blue light by microbes, and a novel interstitial delivery approach of antimicrobial blue light. The potential new applications of antimicrobial blue light are also discussed.

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Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

227

192

View full text Add to dashboard Cite

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“…In vitro inhibition of various bacteria using blue light (405–470 nm) has been frequently demonstrated (Guffey and Wilborn ,b; Guffey et al. ,b,c, ,b,c,d,e,f). To our knowledge, only (Guffey et al.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Salmonella on tainted foods: using blue light to disinfect cucumbers and processed meat products

Guffey

Payne

Motts

et al. 2016

Food Science & Nutrition

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Foodborne illness resulting from infectious organisms occurring in vegetables and processed meat is a serious health concern in the United States. Improved and cost‐effective techniques for disinfection are needed. Visible light in the blue range (405 nm) was administered to processed meat that had been inoculated with Escherichia coli. One application of light energy at doses of 10, 30, 60, and 100 J/cm2 was applied, in vitro. In the case of vegetables contaminated with Salmonella (cucumbers), 464 nm light was used at 6, 12, and 18 J/cm2. In both cases, after 20 hours of incubation, colony‐forming units were counted and compared to controls to determine whether the light energy inhibited growth of E. coli or Salmonella. E. coli – 405 nm light at doses of 30, 60, and 100 J/cm2 were all effective inhibitors of the organism. Kill rates of 75.61 – 96.34% were achieved. Salmonella – 464 nm light at doses of 6, 12, and 18 J/cm2 produced significant inactivation of the organism. Kill rates of 80.23–100% were obtained. Blue light, delivered in the wavelength/dose combinations used in this study is an effective inhibitor of both E. coli and Salmonella on actual foodstuffs. Blue light should be considered as a potentially effective tool in the effort to protect humans from foodborne illnesses.

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“…As Bush et al [4] have pointed out, finding alternative antimicrobial agents/ techniques must be a high-priority focus of current research. To this end, prior to this current experiment, we have been engaged in examining the bactericidal [5][6][7][8][9] and fungicidal [10] effects of visible and infrared light.…”

Section: Introductionmentioning

confidence: 99%

“…Dai, Gupta and Huang et al [2] recently demonstrated 405 nm light was effective in inhibiting P. aeruginosa in vivo related to mouse burn wounds. While higher doses (compared to those needed with S. aureus and P. aeruginosa) of light energy are required for effective inhibition of Mycobacterium smegmatis, blue light is an effective bactericidal agent against this organism [7,11,12]. Visible (primarily blue) light has also been shown to be an effective inhibitor of other microorganisms [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

The Interaction of Dose and Rate of Delivery Using Visible and Infrared Light to Inhibit Pseudomonas aeruginosa In vitro

Guffey

Payne²,

Bednar³

2014

J Laser Opt Photonics

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Background: Visible and near-IR energies have been shown to inhibit the growth of various microorganisms. There may be clinical application for this inhibitory effect in the treatment of infected wounds particularly, and other infectious states generally. Aim: The purpose of this study was to determine whether there was an interactive effect between dose and rate of energy delivery when using combined 464 and 850 nm light to inhibit Pseudomonas aeruginosa. Methods: Solutions containing P. aeruginosa was irradiated with blue and infrared light (464 and 850 nm). The light energy was applied at two different doses and at two different rates of delivery. The doses employed included 9 and 30 Jcm-2. The rates of delivery used were 20 and 125 mWcm-2. Design: An in vitro 2 × 2 factorial design was used to compare doses of 9 and 30 Jcm-2 and rates delivery of 20m Wcm-2 and 125 mWcm-2 to determine kill rates compared to controls. Each dose was applied at each delivery rate. Results: Student t-tests demonstrated (p=0.00) a significant kill rate at three of the four combinations. A two-way analysis of variance identified a significant interactive effect between dose and rate (F=94.717, p=0.00). Tukey's HSD identified 30 Jcm-2 delivered at a 20 mWcm-2 as significantly different from the other combinations. Conclusions: These data suggest that 1) visible and infrared light may be effective inhibitors of P. aeruginosa, and 2) both dose and rate of delivery are important factors to consider when using visible and infrared light energy to inhibit the growth of this organism.

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Inactivation of Candida albicans Following Exposure to 624-Nanometer Light from a Supraluminous Diode Array

Abstract: Appropriate doses of 624-nm light from a supraluminous diode array can inhibit the growth of C albicans in vitro. Three, 9, and 30 J/cm(2) are all effective dose levels.

Cited by 13 publications

References 14 publications

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Inactivation of Salmonella on tainted foods: using blue light to disinfect cucumbers and processed meat products

The Interaction of Dose and Rate of Delivery Using Visible and Infrared Light to Inhibit Pseudomonas aeruginosa In vitro

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