Inactivation of phosphate regulator (SphU) in cyanobacterium Synechocystis sp. 6803 directly induced acetyl phosphate pathway leading to enhanced PHB level under nitrogen-sufficient condition

Inorganic carbon stimulates the metabolic routes related to the polyhdroxybutyrate production in a Synechocystis sp. strain (cyanobacteria) isolated from wastewater

“…As happens in cupriavidus necator, which have more than one copy of the gene phaB and these copies are found in different operons (Silvestrini and Drosg, 2016). Krasaesueb et al (2021), also observed with a ΔsphU Synechocystis sp. PCC 6803 a higher overexpression of phaB in contrast to thl.…”

“…Green shaded reactions indicate the intercorrelated metabolic routes. could be that the PhaC protein is post-transcriptionally regulated (Krasaesueb et al, 2021;Nakaya et al, 2015;Osanai et al, 2013). Further investigation in protein concentrations, including the activities of the different enzymes involved in the PHB and glycogen metabolism and the carbon fluxes towards the pools of ADP-glucose and Acetyl-CoA should be done in the future to confirm this hypothesis.…”

Screening of nanobody targeting alkaline phosphatase PhoX in Microcystis and detection of the PhoX in situ by fluorescence immunoassays

“…Therefore, it was used as a biochemical indicator for P starvation (Zhang et al 2021a). For measuring the activity of APases in phytoplankton, colorimetric and uorometric detection with p-nitrophenyl phosphate (pNPP) and 4-methylumbelliferyl phosphate (4-MUP) substrates, respectively, have been widely employed (Davis and Mahaffey 2017, Krasaesueb et al 2021). Additionally, the extracellular phosphatase in phytoplankton was detected at the single-cell level using an enzyme-labelled uorescence approach (Bar-Yosef et al 2010).…”