Incidence and Relative Prevalence of Distinct Caulimoviruses (Genus Caulimovirus, Family Caulimoviridae) Associated with Dahlia Mosaic in Dahlia variabilis

Pahalawatta, Vihanga; Miglino, R.; Druffel, K. B.; Jodlowska, A.; Schadewijk, A.R. van; Pappu, H. R.

doi:10.1094/pdis-91-9-1194

Cited by 16 publications

(9 citation statements)

References 10 publications

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Section: Introductionmentioning

confidence: 99%

“…Both DCMV and DMV‐D10 were found to be widespread based on surveys conducted in the USA and Europe (Pappu et al. , 2005; Pahalawatta et al. , 2007; Eid et al.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to DMV, it was recently shown that there are at least two more distinct caulimoviruses associated with dahlia. Tentatively referred to as Dahlia common mosaic virus (DCMV) and DMV-D10 (Pahalawatta et al, 2007;Pappu et al, 2008), these two new caulimoviruses showed significant sequence divergence compared to that of DMV (Pahalawatta et al, 2008b;Pappu et al, 2008). Both DCMV and DMV-D10 were found to be widespread based on surveys conducted in the USA and Europe (Pappu et al, 2005;Pahalawatta et al, 2007;Eid et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

Eid¹,

Saar²,

Druffel³

et al. 2010

Plant Pathology

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Selected wild Dahlia species in their natural habitats from west-central Mexico were tested for the presence of three caulimoviruses known to be associated with cultivated dahlia (Dahlia variabilis), viz. Dahlia mosaic virus (DMV), DMV-D10 and Dahlia common mosaic virus. Virus species-specific primers and PCR were used followed by cloning and sequencing of the amplicons. Results showed that the wild dahlia species in their natural habitat contained DMV-D10, which is an endogenous plant pararetrovirus. Viral sequences were found in 91% of the samples (n = 56) representing four different wild species. The gene coding for the movement protein of DMV-D10 from Dahlia coccinea and all other species was cloned and sequenced. Sequence comparisons showed divergence of this gene when compared to that of DMV-D10 from cultivated dahlias. The discovery of plant pararetroviruses in wild dahlia species in their natural habitats suggests a possible emergence, co-existence and co-evolution of pararetroviruses and their host plants.

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Section: Introductionmentioning

confidence: 99%

“…Both DCMV and DMV‐D10 were found to be widespread based on surveys conducted in the USA and Europe (Pappu et al. , 2005; Pahalawatta et al. , 2007; Eid et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

Eid¹,

Saar²,

Druffel³

et al. 2010

Plant Pathology

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“…DMV-D10 shares limited sequence similarity with known caulimoviruses, including DMV, although the genome organization clearly suggests that it is a member of Caulimovirus (Pahalawatta et al, 2008a). DMV-D10 was found to be widespread based on the surveys conducted in the United States and Europe (Pahalawatta et al, 2007;Pappu et al, 2005). Moreover, Pahalawatta et al (2008b) reported that the genome of DMV-D10 is integrated into the dahlia genome and the virus exists as an endogenous plant pararetroviral sequence (EPRV).…”

mentioning

confidence: 99%

“…Previous surveys for the caulimoviruses used partial sequences of DCMV (referred to as DMV-Holland in Pahalawatta et al, 2007) and results showed differences in relative incidence of the three caulimoviruses in the United States and The Netherlands. After the complete genome characterization of DCMV, it becomes evident that there are at least three distinct caulimoviruses that are associated with dahlia mosaic.…”

mentioning

confidence: 99%

Incidence of Multiple and Distinct Species of Caulimoviruses in Dahlia (Dahlia variabilis)

Eid¹,

Druffel²,

Saar³

et al. 2009

horts

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Dahlia mosaic is a serious disease affecting dahlias. In addition to the Dahlia mosaic virus (DMV) reported previously, we characterized two putative new caulimoviruses, tentatively designated as DMV-D10 and Dahlia common mosaic virus (DCMV), from dahlia. To better understand their relative incidence in dahlia, a total of 213 samples were collected during 2007 and 2008 from several varieties of cultivated dahlia (D. variabilis) in the United States. Samples were tested for the three caulimoviruses using virus-specific primers in a polymerase chain reaction. Amplicons were cloned and sequenced to confirm the infection of dahlia with these viruses. Results showed that DMV-D10 was the most prevalent (94%) followed by DCMV (48.5%) and DMV (23%). Mixed infections were common and viruses were detected irrespective of symptom expression at the time of sampling. Two percent of the samples were not infected by any of the three tested caulimoviruses. Results suggest that caulimovirus infections are widespread in dahlia and highlight the need for testing and production of virus-free material to reduce their spread.

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Genome structure and organization of a member of a novel and distinct species of the genus Caulimovirus associated with dahlia mosaic

et al. 2008

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The genome structure and organization of a new and distinct caulimovirus that is widespread in dahlia (Dahlia variabilis) was determined. The double-stranded DNA genome was ca. 7.0 kb in size and shared many of the features of the members of the genus Caulimovirus, such as the presence of genes potentially coding for the movement protein, the inclusion body protein, and the reverse transcriptase (RT), and an intergenic region consisting of a potential 35S promoter. However, the virus differed from the previously described dahlia mosaic caulimovirus and other known caulimoviruses in that the aphid transmission factor (ATF) was absent and the putative coat protein contained a C-terminal deletion and was fused in-frame with the RT. Sequence identity at the amino acid level with known caulimoviruses including a previously reported caulimovirus from dahlia was low and ranged from 32 to 72%. The absence of an ATF and the highly divergent nature of the genomic sequence are characteristics of this new caulimovirus that is widely associated with dahlia.

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Incidence and Relative Prevalence of Distinct Caulimoviruses (Genus Caulimovirus, Family Caulimoviridae) Associated with Dahlia Mosaic in Dahlia variabilis

Cited by 16 publications

References 10 publications

Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

Incidence of Multiple and Distinct Species of Caulimoviruses in Dahlia (Dahlia variabilis)

Genome structure and organization of a member of a novel and distinct species of the genus Caulimovirus associated with dahlia mosaic

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