Inclusion complexes of cysteinyl β‐cyclodextrin with baicalein restore collagen synthesis in fibroblast cells following ultraviolet exposure

Abstract

Baicalein, a bioactive flavonoid, has poor water solubility, thereby limiting its use in a wide range of biological applications. In the present study, we used inclusion complexes of cysteinyl β-cyclodextrin (β-CD) with baicalein to enhance the stability and solubility of baicalein in aqueous solution. We examined the effects of inclusion complexes of cysteinyl β-CD on collagen synthesis following ultraviolet (UV) irradiation, as well as the mechanisms underlying its effects. Our findings demonstrated that baicalein significantly restored collagen synthesis in the UVexposed human fibroblast Hs68 cells. In addition, synthetic cysteine functionalized β-CDs were found to promote baicalein-induced collagen synthesis. Inclusion complexes of cysteinyl β-CDs with baicalein significantly upregulated the protein expression of type I collagen and activated the transcription of type I, II, and III collagen. Inclusion complexes of cysteinyl β-CDs with baicalein also downregulated matrix metalloproteinase -1 and -3, and α-smooth muscle actin expression. In addition, inclusion complexes of cysteinyl β-CDs with baicalein attenuated the expression of caveolin-1, but this treatment enhanced the UV-induced phosphorylation of Smad in the transforming growth factor-β pathway. These results suggested that the newly synthesized derivative of CD can be used as a complexing agent to enhance the bioavailability of flavonoids such as baicalein, especially in restoring collagen synthesis.baicalein, collagen, cysteinyl β-cyclodextrin (β-CD), inclusion complex, ultraviolet-exposed fibroblast 1 | INTRODUCTION Collagen plays an important role in maintaining the skin and is largely responsible for its strength and resilience. Skin aging is inevitably associated with disturbances in collagen metabolism, such as decreased synthesis and increased degradation. 1 Skin aging is influenced by both intrinsic (genetics) and extrinsic factors (environmental exposure). Ultraviolet (UV) irradiation is particularly important extrinsic risk factor. 2 Skin aging involves the degradation of dermal collagen fibrils in the extracellular matrix by matrix metalloproteinases (MMPs). 3 UV irradiation of the connective tissue in the skin induces the production of MMP-1, MMP-3, and MMP-9 in the normal human skin in vivo. This leads to collagen degradation, especially of type I procollagen (COLI), the J Cell Biochem. 2019;120:4032-4043. wileyonlinelibrary.com/journal/jcb 4032 |

Retracted: Baicalein retards proliferation and collagen deposition by activating p38MAPK‐JNK via microRNA‐29

“…Baicalein (BAI) is a phenolic flavonoid compound and a component of Scutellaria baicalensis Georgi . BAI has been proved to possess multiple bioactive efficacies, especially in modulating collagen level in fibroblast cells 11 . Besides, a study suggested that BAI could attenuate the formation of the hypertrophic scar by inactivating signaling pathways 12 .…”

“…BAI has been proved to possess multiple bioactive efficacies, especially in modulating collagen level in fibroblast cells. 11 Besides, a study suggested that BAI could attenuate the formation of the hypertrophic scar by inactivating signaling pathways. 12 Furthermore, BAI has been confirmed to mediate miRs expression in several diseases, for example, pulmonary fibrosis and cancers [13][14][15] while extensive quantitative data on the formation of scar via mediating miR-29 have so far been sparse.…”

Review of the Usability of Cyclodextrin as a Cosmetic Ingredient

“…Lee et al, 2019 http://dx.doi.org/10.20402/ajbc.2019.0314 국문초록 사이클로덱스트린(Cyclodextrin)의 화장품 유효성분으로서의 유용성 고찰 바깥에 위치하며 외부환경의 영향은 피부노화의 원인이 되기도 한다. 화장품을 이용한 피부의 노화 지연을 위 한 노력에 있어서 화장품 성분의 안정화 기술 방법은 그 기능을 극대화 시키는데 있어서 중요하다.…”