Inconsistent Expression of Glycolipid Sulfotransferase Activity between Hepatoma and Serum.

Conversion of short‐chain ceramides to short‐chain ceramide GM3 in B16 melanoma cells

“…C6-Cer GM3 and C6-Cer cerebroside were prepared from lyso-GM3 and psychosine, respectively, by the method as shown in ref. [14]. Lyso-GM3 was prepared from GM3 by digestion with sphingolipid ceramide N-deacylase [10].…”

Enzymatic Synthesis of ω-Amino-Ceramide: Preparation of a Sensitive Fluorescent Substrate for Ceramidase

“…To date, the literature on the preparation of -amino-sphingolipids seems to be restricted to -aminoglycosphingolipids using purely chemical procedures (10,13). In comparison with the methods reported previously, the present method using SCDase is novel and much easier and gives a high yield.…”

“…N-Trifluoroacetylation of the -aminododecanoic acid was carried out according to the method described in (10).…”

Role of sulfatide in normal and pathological cells and tissues

“…Interaction of chemokine ligand 12 (CXCL12) with CXCR4 induces chemostasis for leukocytes ( 90 ). Sulfatide is thought to contribute to migration elevated compared with that in healthy subjects ( 103 ), although the CST level of hepatocarcinoma tissues is similar to that in normal controls ( 104 ). For human renal cell carcinoma cell lines, marked increases of CST mRNA and CST activity were observed in six cell lines (SMKT-R1, SMKT-R2, SMKT-R3, SMKT-R4, TOS-1, and TOS-2), whereas ACHN cells showed only a slight increase, as compared with normal cells ( 105 ).…”