Incorporation of C18 polyunsaturated fatty acids into L cell phospholipids under normal conditions and during infection with Venezuelan equine encephalitis virus

Cowen, Wm F.; Heydrick, Fred P.

doi:10.1016/0014-4827(72)90002-x

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Addition of some fatty acids to cells in culture increases lipid synthesis (Cowen and Heydrick, 1972;Burkhardt, 1991). Conversely, unsaturated fatty acids, such as oleic acid, increase membrane fluidity and diminish the infectivity and hemolytic activity of enveloped animal virus particles.…”

Section: Oleic Acidmentioning

confidence: 99%

Picornavirus inhibitors

Carrasco

1994

Pharmacology & Therapeutics

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Picornaviruses are among the best understood animal viruses in molecular terms. A number of important human and animal pathogens are members of the Picornaviridae family. The genome organization, the different steps of picornavirus growth and numerous compounds that have been reported as inhibitors of picornavirus functions are reviewed. The picornavirus particles and several agents that interact with them have been solved at atomic resolution, leading to computer-assisted drug design. Picornavirus inhibitors are useful in aiding a better understanding of picornavirus biology. In addition, some of them are promising therapeutic agents. Clinical efficacy of agents that bind to picornavirus particles has already been demonstrated.

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Section: Oleic Acidmentioning

confidence: 99%

Picornavirus inhibitors

Carrasco

1994

Pharmacology & Therapeutics

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“…More specific information on this point could be obtained by measuring cell division and associated processes such as phospholipid and DNA synthesis in the first days after hepatectomy (8). This information cannot be provided by studies with cultured cells since no cell lines specifically requiring EFA have yet been demonstrated (27)(28)(29). Thus, hepatectomy in EFA-deficient rats is a potentially important method of testing for specific functions of EFA in cell division.…”

mentioning

confidence: 98%

Responses of Essential Fatty Acid-Deficient Rats to Fasting-Refeeding and Partial Hepatectomy

Williams

Waldeck

Ojakian

et al. 1977

Experimental Biology and Medicine

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The proportion of arachidonate in liver phospholipids in young rats decreases from about 30 to 10-12% after 4 weeks of feeding an essential fatty acid-deficient diet (1-3). Subsequently, arachidonate decreases only slowly (3)(4)(5), and turnover of liver phospholipids appears reduced ( 5 ) . It is generally assumed that a certain level of essential fatty acids (EFA) in phospholipids is required for membrane integrity, function, and formation (6). Reduced phospholipid turnover ( 5 ) might be one consequence of a decrease in EFA in membrane phospholipids.Information on the need for EFA-containing phospholipids in membrane structure, as well as on the ability of EFA-depleted liver to form phospholipids, could be obtained by subjecting EFA-deficient rats to stimuli which normally increase phospholipid formation. Little or no response would occur if a relatively high proportion of EFA were required. Considerable response would occur if nonessential unsaturated fatty acids could replace essential fatty acids to a large extent in these functions. Stimulation of liver phospholipid formation could also produce even greater depletion of liver EFA than is usually obtained in EFA-deficient rats.Two conditions which stimulate liver phospholipid formation are (a) partial hepatectomy (7, 8) and (b) refeeding of a high carbohydrate diet after fasting (9-1 1). Partial hepatectomy normally produces liver hyperplasia and hypertrophy which require formation of new phospholipid-containing membranes (8). Refeeding after fasting increases lipoprotein secretion (9). Park et al.(1 1 ) reported that fasting-refeeding also decreased the concentrations of arachidonate and linoleate in microsomal phospholipids, To whom all correspondence should be addressed.presumably as a result of increased lipoprotein secretion.Decreases in phospholipid EFA may underlie increased activities of liver glucose-6phosphate dehydrogenase (G6PD) and other lipogenic enzymes in rats refed high carbohydrate diets after fasting (10, 11). The activities of liver lipogenic enzymes are already elevated in EFA-deficient rats fed ad libitum ( 1 ) . Thus, these enzyme activities might become even higher if additional decreases in phospholipid EFA were produced by fasting-refeeding of EFA-deficient rats.In the present report, we have subjected EFA-deficient rats to fasting-refeeding or to partial hepatectomy and then have measured liver phospholipid fatty acid composition and recovery of liver and body weights. Activities of glucose-6-phosphate dehydrogenase (G6PD) and fatty acid synthetase (FAS) were also measured in the fastedrefed deficient rats.Materials and methods. Male weanling Long-Evans or Sprague-Dawley rats (Simonsen Farms, Gilroy, Calif.) were caged individually in galvanized screen wire-bottom cages and were fed ad libitum either a fat-free diet or the same diet supplemented with 5 % safflower oil (1). The oil replaced an equal weight of sucrose. In the fastingrefeeding study, rats were fed the fat-free diet ad libitum for 7 weeks after weaning, then fasted fo...

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“…El desarrollo neuronal del sistema nervioso de los mamíferos comprende una sucesión de fenómenos interrelacionados entre sí, que clásicamente se dividen en las siguientes fases: proliferación neuronal o neurogénesis, migración neuronal, agregación neuronal, diferenciación celular, sinaptogénesis y, por último muerte celular (Cowan 1987;Cowen and Heydrick 1972;Herschkowitz 1988).…”

Section: Desarrollo Neuronal Del Sistema Nervioso Centralunclassified

“…En algunos casos se habla de FABPs (nombre referido a su función: "fatty acid binding proteíns") para referirse, de una manera general, tanto a proteínas que unen lípidos a nivel intracelular (FABP C ), como a proteínas translocadoras asociadas a la membrana plasmática (FABP PM ) (Chmurzynska 2006). Sin embargo, esta denominación no es totalmente correcta y el término FABPs suele utilizarse únicamente para una familia de proteínas citoplasmáticas de bajo peso molecular, encargadas de la unión y el transporte intracelular de lípidos (Cowen and Heydrick 1972;Ockner et al 1972). Veerkamp y Maatman (Veerkamp and Maatman 1995a) establecieron una clasificación en tres grandes grupos, para referirse a las familias de proteínas de unión y transporte de lípidos no unidas a membranas (Ver Tabla 1.1), en la que se aprecia cómo las FABPs son, propiamente, un subgrupo dentro una de estas categorías.…”

Section: Proteínas Transportadoras De áCidos Grasosunclassified

Papel de las proteínas transportadoras de ácidos grasos (FABPs), en la señalización del efecto neurotrófico del ácido oleico en neuronas

Martin¹,

Alberto²

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AUTORIZANLa presentación de la Tesis Doctoral: "Papel de las proteínas transportadoras de ácidos grasos (FABPs), en la cadena de señalización del efecto neurotrófico del ácido oleico en neuronas", realizada bajo su dirección por el Licenciado en Y para que conste, firman el documento, en Salamanca a 09 de Julio de 2012. Fdo. José María Medina Jiménez Fdo. Aránzazu Tabernero Urbieta ILMO SR. DR. DEPARTAMENTO DE BIOQUÍMICA Y BIOLOGÍA MOLECULAR DE LA UNIVERSIDAD DE SALAMANCA ratos de café. A André, por sus enseñanzas y recomendaciones. A Lourdes, a quien no conocí hasta mucho tiempo después de llevar intercambiados numerosos correos, por su energía, y su ayuda y consejos durante la continuación de su trabajo de Alzheimer. A los miembros de la línea de las conexinas, Teresa, Sandra y José Carlos, por los buenos ratos pasados. Y por último, al "reciente" doctor, a Alex, el jedi que me tuvo como padawan brevemente, y con el que he compartido muchas horas de laboratorio y he vivido los momentos buenos y aquellos en los que no salen las cosas. My gratitude to Prof. Hannah Monyer from Klinische Neurobiologie des Universitätsklinikums -Deutsches Krebsforschungszentrum (DKFZ), at Heidelberg, for the opportunity to be in her lab and the chance to learn new techniques, and participate in her research projects and interesting lab meetings. Special thanks to Dr. Julieta Alfonso and Dr. Corentin Le Magueresse for helping me during my stay and their guidance in the work. Thank you, all of you, for your help and the meal's times during my stay. Thank you so much, Dr. Anne Herb and Ms Laura Winkel for making me easy the incorporation to the laboratory and helping with the administrative requirements. Thank you, Ms Irmgard Preugschat-Gumprecht and Ms Regina Hinz-Herkommer, both of you,made me feel like at home during my stay in the lab. A los compañeros del laboratorio 102, ahora integrantes del 122 y 128, futuros integrantes del IBFG, a los que todavía siguen y a los se encuentran repartidos por múltiples laboratorios. A la Dra. María Delgado, a Nacho, Ángel, Rubén, Mónica, Patricia, Seila, Verónica,… por los momentos vividos, especialmente en las cenas y los congresos. A los compañeros de Biología Celular, Carmela, Gloria, Fernando, Azucena, David, Miguel, y todos los que me dejo en el tintero, por toda la ayuda prestada y los ratos fuera del trabajo. A Verónica, del laboratorio 13, por sus palabras y consejos dentro y fuera del INCyL. A los miembros del racimo de bioquímica, Raquel, Julia, Mercedes, Ariadna, Pilar y Néstor, por las risas, los viajes y los cafés. A los amigos, los que me han soportado durante ya bastante tiempo, en los buenos y malos momentos. A Ro. A Noe, Pablo y Marta. A Olga (best-friend ever), a pesar de que me llames viejo, y porque las clases con D. Julio y las horas compartidas son ya muchas. A Jesús por toda su ayuda y sus consejos, por los cafés y las noches de póker a las que se incorporaba María, la hermana de uno de los siguientes. A esos dos caballeros de escapadas y vivencias, que me conocen desde ha...

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Incorporation of C18 polyunsaturated fatty acids into L cell phospholipids under normal conditions and during infection with Venezuelan equine encephalitis virus

Cited by 7 publications

References 12 publications

Picornavirus inhibitors

Picornavirus inhibitors

Responses of Essential Fatty Acid-Deficient Rats to Fasting-Refeeding and Partial Hepatectomy

Papel de las proteínas transportadoras de ácidos grasos (FABPs), en la señalización del efecto neurotrófico del ácido oleico en neuronas

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